FastQCFastQC Report
Wed 25 May 2016
SRR1294895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873165
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60970.21220500736992132No Hit
CCCATGTACTCTGCGTTGATACCAC57870.20141551216167536No Hit
GTATCAACGCAGAGTACTTTTTTTT37930.13201469459637716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC500.00150163213.2981836
GGACCGC600.00588369211.081826
TAGACAG4800.010.4899785
CTGTCGC2500.09.4985379
GTATTAG6050.09.2748281
GTCTTAC2401.0913936E-119.1143531
GTCGCCC2201.6916601E-109.06694311
CCCCTAT1901.03864295E-89.010071
GTCCTAA2451.6370905E-118.9283451
GTCTTGC4950.08.83816051
TGTCGCC2701.8189894E-128.795094510
GTCATAC1752.5825102E-78.6954331
TCTACAC3750.08.6145573
GACTGTC2554.0017767E-118.5673077
CTAACAC3550.08.5645973
TCGCCCA2356.184564E-108.48820212
TCTGTCG2356.202754E-108.4880548
GTATTAA6500.08.4864071
GCCTAAT1803.9682527E-78.4538931
TTAGACA4300.08.3965434
Produced by FastQC (version 0.11.5)