FastQCFastQC Report
Wed 25 May 2016
SRR1294895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873165
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88960.3096237076534066No Hit
GTACATGGGAAGCAGTGGTATCAAC63040.21940960578316943No Hit
CCCATGTACTCTGCGTTGATACCAC62590.21784338873681114No Hit
GGTATCAACGCAGAGTACTTTTTTT56400.19629920314357163No Hit
TATCAACGCAGAGTACTTTTTTTTT49050.17071765805305297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA658.031315E-411.69156910
AGTACCG600.005877436611.0834045
CTAGGAC2900.010.1552373
GTACTAG2303.6379788E-129.5061821
TAGGACC1804.1854946E-99.5000614
TCTGTCG1606.647497E-89.4993998
CACACGA900.00111476379.49939914
TAGGACT3450.09.0870154
GTCTAAT2950.09.0228181
TGGACCG850.00743757378.9412345
CGCTCTG1701.6860213E-78.9412345
GTCTAGG3200.08.9120461
TAGACAG5050.08.841645
CTAGTAC1951.6500053E-88.7692863
CTAGCAC2850.08.6667223
GTCTTAC3100.08.5862291
ATAAGAC3500.08.414343
TAAGACT3400.08.3824064
TTATACT6350.08.3780064
TAGGGTC1252.7442104E-48.3600535
Produced by FastQC (version 0.11.5)