FastQCFastQC Report
Wed 25 May 2016
SRR1294894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC134580.3021291823384862No Hit
CCCATGTACTCTGCGTTGATACCAC120550.27063213650545775No Hit
GAGTACATGGGAAGCAGTGGTATCA57460.128996454281241No Hit
GTATCAACGCAGAGTACTTTTTTTT55680.12500039287120604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC658.037825E-411.6907037
CGTGCGC600.00588507511.08168810
GGACCGT1054.115082E-59.9510156
ACTGACG800.00452335839.4986968
TACCGTG1554.2745341E-79.1922877
TATACTG9600.09.0051875
TACACTG11600.08.9267035
GTGCTAG5950.08.7932511
GTCTTAG5000.08.5614291
TAGACAG5900.08.5338785
TGGACCG1606.4564447E-68.312485
GTCCTAT4400.08.2155121
GTGTAGA6950.08.2124021
CTTAGAC3950.08.1777473
TAAACCG1050.00451536158.14283855
GGTGCGC1050.00452084758.141748
GTCCTAG4350.08.0912621
GTATTAG7300.08.0792791
GTTCTAG5300.08.0768191
GTCTAGT3550.08.03891
Produced by FastQC (version 0.11.5)