FastQCFastQC Report
Wed 25 May 2016
SRR1294894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT133300.2992556100885734No Hit
CCCATGTACTCTGCGTTGATACCAC132830.2982004702780585No Hit
GTACATGGGAAGCAGTGGTATCAAC132090.29653918632107773No Hit
GGTATCAACGCAGAGTACTTTTTTT84550.18981291697666075No Hit
TATCAACGCAGAGTACTTTTTTTTT74800.16792437835427823No Hit
GAGTACATGGGAAGCAGTGGTATCA59810.13427215333381526No Hit
CATGTACTCTGCGTTGATACCACTG46070.10342615121365774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG751.4793402E-512.6672585
CGCAAAC600.00588144611.08260712
GACCGTA803.7759417E-410.6867997
CCGTGCA1151.0283193E-59.9123949
ACAGCGC1951.6370905E-109.742958
CTTACAC6250.09.7285633
GTCTTAC4800.09.5044981
GGGCCGA800.0045165059.5004436
CCTACAC5400.09.1486783
AGACCGG950.00182407459.000426
TGGACCG850.00743645578.9415935
GTCCTAT3550.08.8351671
GTATTAA9200.08.781331
AGACCGT1752.6268935E-78.6861196
TATACCG1107.145605E-48.6367675
CCCGTCC1107.153589E-48.63579758
TGAACCG1000.0029053768.55045
GTAGGAC2606.002665E-118.4043333
GTCTTGC7200.08.3164361
GTGCTAG5750.08.264781
Produced by FastQC (version 0.11.5)