FastQCFastQC Report
Wed 25 May 2016
SRR1294893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4061542
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC62810.15464569860412622No Hit
CCCATGTACTCTGCGTTGATACCAC58740.14462487400105672No Hit
GTATCAACGCAGAGTACTTTTTTTT48270.11884648736859055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5350.010.6683331
GTCGTAT1105.9568774E-610.3773791
ACGGGTC1054.1061532E-59.95292955
TTGACGC2151.0913936E-119.72014817
AGTACCG2054.2382453E-109.2688055
GTTCTAA3250.09.0735541
CTAGGAC3400.08.9426613
CGCTCAA850.00744848848.93979613
CGTATCC1606.732407E-78.9077283
GACGCTT2356.548362E-118.8933419
TAGGACT3850.08.8844824
TCGTATC1502.698398E-68.8681392
TACGACA1304.364364E-58.7705794
GTATTAA7400.08.7413051
TACCGCC1856.603841E-88.728067
GTCCTAA4150.08.710331
TAGACTG3950.08.6587055
GTGTTAG4900.08.54192351
TAGACAG4900.08.5310825
ATTGCGA1356.818388E-58.4428298
Produced by FastQC (version 0.11.5)