FastQCFastQC Report
Wed 25 May 2016
SRR1294893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4061542
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119440.2940755013736162No Hit
GGTATCAACGCAGAGTACTTTTTTT72260.1779127237881573No Hit
TATCAACGCAGAGTACTTTTTTTTT65700.16176122270802568No Hit
GTACATGGGAAGCAGTGGTATCAAC63250.15572903099364724No Hit
CCCATGTACTCTGCGTTGATACCAC62480.15383319931198547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA551.9624997E-413.817259
GTACGAC700.001491913210.857483
ATTTGCG803.775849E-410.6867811
TATGACG1201.5256173E-610.2916074
TCTAGAC3700.010.0138253
ACTACCG800.0045183159.4999455
TGTACCG1304.2433094E-69.4999455
TATCGCT900.00111438739.4999454
ACCGACC800.004520619.499368
ATTCGAT800.004520619.4993611
ACCGTTA800.004520619.499368
GGACCGG2357.2759576E-129.2978186
GACCGGG2252.7284841E-119.2882627
AGACCGT1651.0671829E-79.2120686
GTCTAGA4850.09.21180251
TACACCG950.00182487428.9999485
GACCGCG950.00182589668.9993937
CGGGAAT1804.2293323E-88.97161810
TAGAACC2651.8189894E-128.9622124
GTCGTGC850.0074431838.9405748
Produced by FastQC (version 0.11.5)