FastQCFastQC Report
Wed 25 May 2016
SRR1294892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862180
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50050.12959002428680175No Hit
GTACATGGGAAGCAGTGGTATCAAC41930.10856562873817377No Hit
CCCATGTACTCTGCGTTGATACCAC38960.10087567125302291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC550.003074571712.08843510
GTACCGT951.0443182E-611.9969236
ACCGTAC600.005889564311.0804918
TATCCCG750.00264988810.1329675
TAGACAG6400.09.9449535
AGTCCGA850.00744113568.94085317
TTAGACA5800.08.8459994
GTATTAG6800.08.8153191
CGCTCTG3350.08.7907275
TTAGGCT3600.08.7094854
AGGACCG1000.00290737428.5496915
GTATTAA7250.08.53064251
TCTGGAC4250.08.49513153
TAACACG1356.792372E-58.4455624
ATAGGAC3600.08.4454533
TGCGTTG11550.08.38789512
GTCCTGG7400.08.3577241
CTTATAC5650.08.2399223
GTCTCGC3500.08.1556691
GTCTAAG3500.08.1556691
Produced by FastQC (version 0.11.5)