FastQCFastQC Report
Wed 25 May 2016
SRR1294892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3862180
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT117230.30353323770513024No Hit
GGTATCAACGCAGAGTACTTTTTTT76930.19918802334432886No Hit
TATCAACGCAGAGTACTTTTTTTTT64470.16692645086453764No Hit
GTACATGGGAAGCAGTGGTATCAAC43190.11182803494399537No Hit
CCCATGTACTCTGCGTTGATACCAC40230.10416396957158909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC350.002171923616.284668
CGGTTTG600.0058811711.08261614
AGGACTG6250.09.5763725
GCACCGT1106.8388516E-59.5003696
GGGCCGT800.0045165659.5003696
AACACCG1304.2406973E-69.5003695
GGTATCA33800.09.1954721
GTCCTAC2701.8189894E-128.800741
TATATCG1201.764232E-48.7086725
GTACTAG2553.8198777E-118.5729561
AGAACCG1000.00290540378.5503325
TAGGACT5250.08.5050934
CTTATAC5200.08.404393
GTATCAA55050.08.3738921
GTCTAGG4450.08.3300491
TTATACT6850.08.3214914
CTAGAAC4800.08.3130383
CTTACAC5850.08.2825883
AGACCGT1150.00111075548.261196
GTCCTAA3800.08.2541681
Produced by FastQC (version 0.11.5)