FastQCFastQC Report
Wed 25 May 2016
SRR1294891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365088
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC199945.476487860461039No Hit
CCCATGTACTCTGCGTTGATACCAC194325.3225523709352265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA148634.071073275484267No Hit
GAGTACATGGGAAGCAGTGGTATCA96282.637172407748269No Hit
CATGTACTCTGCGTTGATACCACTG76592.0978503812779383No Hit
GCGTTGATACCACTGCTTCCCATGT65111.7834056446664914No Hit
ACGCAGAGTACATGGGAAGCAGTGG59651.633852660180559No Hit
TATCAACGCAGAGTACATGGGAAGC58051.5900276097817512No Hit
GGTATCAACGCAGAGTACATGGGAA57961.5875624506968185No Hit
GTATCAACGCAGAGTACATGGGAAG56601.5503111578578315No Hit
ACTCTGCGTTGATACCACTGCTTCC50301.3777500219125252No Hit
GCTTCCCATGTACTCTGCGTTGATA37951.0394754141467262No Hit
GTGGTATCAACGCAGAGTACATGGG36270.9934591112279779No Hit
GTACTCTGCGTTGATACCACTGCTT34760.952099219914103No Hit
ATACCACTGCTTCCCATGTACTCTG30080.8239109474975896No Hit
GATACCACTGCTTCCCATGTACTCT27970.7661166622841616No Hit
GGGAAGCAGTGGTATCAACGCAGAG27170.7442041370847576No Hit
CTGCTTCCCATGTACTCTGCGTTGA25720.7044876851608379No Hit
CAGTGGTATCAACGCAGAGTACATG25310.6932575159961434No Hit
CATGGGAAGCAGTGGTATCAACGCA25190.6899706372162329No Hit
GCAGAGTACATGGGAAGCAGTGGTA23780.6513498115522833No Hit
GTTGATACCACTGCTTCCCATGTAC22950.6286155666579016No Hit
TACCACTGCTTCCCATGTACTCTGC22720.622315715663073No Hit
GCAGTGGTATCAACGCAGAGTACAT22280.6102638268034007No Hit
ACATGGGAAGCAGTGGTATCAACGC21520.589446927863967No Hit
CTTCCCATGTACTCTGCGTTGATAC20540.5626040844946971No Hit
CCACTGCTTCCCATGTACTCTGCGT19600.5368568673853975No Hit
CCATGTACTCTGCGTTGATACCACT18240.49960557454641075No Hit
GTATCAACGCAGAGTACTTTTTTTT17420.47714523621702165No Hit
AAGCAGTGGTATCAACGCAGAGTAC17160.47002366552721536No Hit
ATGGGAAGCAGTGGTATCAACGCAG16540.4530414584976773No Hit
TGATACCACTGCTTCCCATGTACTC15010.4111337540538172No Hit
TTCCCATGTACTCTGCGTTGATACC14330.39250810763432376No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA13950.3820996581646069No Hit
GGTATCAACGCAGAGTACTTTTTTT13760.37689543342974846No Hit
ATCAACGCAGAGTACATGGGAAGCA13190.3612827592251731No Hit
GTACATGGTAAGCAGTGGTATCAAC11900.3259488123411342No Hit
TATCAACGCAGAGTACTTTTTTTTT10790.2955451836269612No Hit
ACCATGTACTCTGCGTTGATACCAC9750.26705890086773604No Hit
GGAAGCAGTGGTATCAACGCAGAGT9680.2651415549127881No Hit
AAAAAGTACTCTGCGTTGATACCAC8000.2191252519940398No Hit
AAACAAAAAAAAAAAAAAAAAAAAA7510.20570383030940487No Hit
CTGCGTTGATACCACTGCTTCCCAT7320.2004996055745464No Hit
TGGGAAGCAGTGGTATCAACGCAGA6920.18954334297484443No Hit
TCAACGCAGAGTACATGGGAAGCAG6760.18516083793496363No Hit
CCCCATGTACTCTGCGTTGATACCA6630.18160005259006048No Hit
CTCTGCGTTGATACCACTGCTTCCC6560.17968270663511263No Hit
GGTAAGCAGTGGTATCAACGCAGAG6030.1651656586905075No Hit
TCCATGTACTCTGCGTTGATACCAC6000.16434393899552985No Hit
AAAGTACTCTGCGTTGATACCACTG5650.15475720922079061No Hit
AGTGGTATCAACGCAGAGTACATGG5560.15229205013585764No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5460.14955298448593216No Hit
TCCCATGTACTCTGCGTTGATACCA5350.1465400122710141No Hit
GTACATGGGTAAGCAGTGGTATCAA5090.13941844158120784No Hit
ATGTACTCTGCGTTGATACCACTGC4980.13640546936628978No Hit
GTACATGGAAAGCAGTGGTATCAAC4890.13394031028135683No Hit
AACGCAGAGTACATGGGAAGCAGTG4850.13284468402138663No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA4730.129557805241476No Hit
GAGTACATGGTAAGCAGTGGTATCA4730.129557805241476No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4710.12900999211149092No Hit
ACTGCTTCCCATGTACTCTGCGTTG4700.12873608554649837No Hit
GCTTACCATGTACTCTGCGTTGATA4380.1199710754667368No Hit
GAACAAAAAAAAAAAAAAAAAAAAA4230.11586247699184854No Hit
AAAAACAAAAAAAAAAAAAAAAAAA4060.1112060653869752No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4020.110110439127005No Hit
TTGATACCACTGCTTCCCATGTACT3930.10764528004207205No Hit
CAACGCAGAGTACATGGGAAGCAGT3690.10107152248225086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGG250.0059931219.0165981
CGCAAAA508.681108E-515.20076752
GTAAAAA704.3655928E-714.941611
ACGCAAA551.9392675E-413.8302511
GTACAAA903.567584E-813.7342081
GAACAAA2750.012.4472261
CGCAGAA550.003059558312.0915192
TACAAAA1459.640644E-1111.7936982
AAAAGTA1303.80247E-710.2312862
AGAACAA856.5641716E-410.0593322
GAGAACA1054.0418745E-59.9610741
AAAAAGT1401.0361582E-69.5082981
GAAAAAA8600.09.2871751
GCAAAAA1252.7332262E-59.12046053
GAAACAA1304.122404E-48.0454831
AGTACTC1701.440159E-57.82285265
AAGTACT1752.1145628E-57.60038384
AAAGTAC1752.1145628E-57.60038383
TGGTAAG3900.07.30605846
CCATGTA57300.07.29530762
Produced by FastQC (version 0.11.5)