FastQCFastQC Report
Wed 25 May 2016
SRR1294891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365088
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC210655.76984179156806No Hit
GTACATGGGAAGCAGTGGTATCAAC201905.530173547199579No Hit
GAGTACATGGGAAGCAGTGGTATCA97802.6788062056271364No Hit
CATGTACTCTGCGTTGATACCACTG78602.152905600841441No Hit
GCGTTGATACCACTGCTTCCCATGT68301.8707818388991146No Hit
ACGCAGAGTACATGGGAAGCAGTGG68101.8653037075992636No Hit
GTATCAACGCAGAGTACTTTTTTTT65271.7877881497063721No Hit
TATCAACGCAGAGTACATGGGAAGC59561.6313875010956262No Hit
GTATCAACGCAGAGTACATGGGAAG57871.5850972916118853No Hit
GGTATCAACGCAGAGTACATGGGAA57061.5629108598474888No Hit
ACTCTGCGTTGATACCACTGCTTCC54391.489777806994478No Hit
GGTATCAACGCAGAGTACTTTTTTT52061.4259575773512139No Hit
TATCAACGCAGAGTACTTTTTTTTT46091.2624353580506618No Hit
GCTTCCCATGTACTCTGCGTTGATA39361.0780962398106757No Hit
GTGGTATCAACGCAGAGTACATGGG35590.9748334648084845No Hit
GTACTCTGCGTTGATACCACTGCTT33110.9069046366903323No Hit
GATACCACTGCTTCCCATGTACTCT29820.8167893768077833No Hit
ATACCACTGCTTCCCATGTACTCTG29690.8132285914628801No Hit
GGGAAGCAGTGGTATCAACGCAGAG29270.8017245157331931No Hit
CATGGGAAGCAGTGGTATCAACGCA28420.7784424577088264No Hit
CAGTGGTATCAACGCAGAGTACATG27910.7644732228942064No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27220.7455736699097204No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26850.735439127004996No Hit
CTGCTTCCCATGTACTCTGCGTTGA25520.699009553860987No Hit
ACATGGGAAGCAGTGGTATCAACGC24600.6738101498816724No Hit
GCAGAGTACATGGGAAGCAGTGGTA24460.6699754579717766No Hit
GCAGTGGTATCAACGCAGAGTACAT23460.6425848014725217No Hit
TACCACTGCTTCCCATGTACTCTGC23130.6335458848277676No Hit
GTTGATACCACTGCTTCCCATGTAC20930.5732864405294067No Hit
CTTCCCATGTACTCTGCGTTGATAC20750.5683561223595407No Hit
CCACTGCTTCCCATGTACTCTGCGT20740.5680822157945482No Hit
CCATGTACTCTGCGTTGATACCACT20060.5494565693750548No Hit
ATGGGAAGCAGTGGTATCAACGCAG19100.52316153913577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18800.5149443421859935No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18710.5124791831010606No Hit
AAGCAGTGGTATCAACGCAGAGTAC17680.48426680690682794No Hit
TTCCCATGTACTCTGCGTTGATACC15110.4138728197037427No Hit
TGATACCACTGCTTCCCATGTACTC15030.4116815671838023No Hit
ATCAACGCAGAGTACATGGGAAGCA14250.3903168551143834No Hit
GTACATGGTAAGCAGTGGTATCAAC12690.3475874309755456No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11150.30540581996669297No Hit
ACCATGTACTCTGCGTTGATACCAC9990.2736326584275572No Hit
GGAAGCAGTGGTATCAACGCAGAGT9900.27116749934262424No Hit
GTGGTATCAACGCAGAGTACTTTTT9430.2582938907879744No Hit
ATCAACGCAGAGTACTTTTTTTTTT7790.21337321412919627No Hit
TGGGAAGCAGTGGTATCAACGCAGA7780.21309930756420373No Hit
TCAACGCAGAGTACATGGGAAGCAG7750.21227758786922604No Hit
AAAAAGTACTCTGCGTTGATACCAC7560.2070733631343676No Hit
CCCCATGTACTCTGCGTTGATACCA7490.20515601717941973No Hit
CTGCGTTGATACCACTGCTTCCCAT7440.203786484354457No Hit
CTCTGCGTTGATACCACTGCTTCCC7110.19474756770970286No Hit
TCCATGTACTCTGCGTTGATACCAC6330.17338285564028397No Hit
GGTAAGCAGTGGTATCAACGCAGAG6250.1711916031203436No Hit
TCCCATGTACTCTGCGTTGATACCA5790.1585919011306863No Hit
AAAGTACTCTGCGTTGATACCACTG5670.1553050223507757No Hit
AACGCAGAGTACATGGGAAGCAGTG5560.15229205013585764No Hit
GTACATGGAAAGCAGTGGTATCAAC5240.14352704005609607No Hit
AGTGGTATCAACGCAGAGTACATGG5230.14325313349110352No Hit
GTACATGGGTAAGCAGTGGTATCAA5150.1410618809711631No Hit
ATGTACTCTGCGTTGATACCACTGC5010.1372271890612674No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA5000.13695328249627486No Hit
GAGTACATGGTAAGCAGTGGTATCA4880.1336664037163643No Hit
ACTGCTTCCCATGTACTCTGCGTTG4340.11887544920676658No Hit
GCTTACCATGTACTCTGCGTTGATA4310.11805372951178895No Hit
CAACGCAGAGTACATGGGAAGCAGT4270.11695810325181874No Hit
TTGATACCACTGCTTCCCATGTACT3990.10928871943202735No Hit
ACTCTGCGTTGATACCACTGCTTAC3730.10216714874222105No Hit
CGCAGAGTACATGGGAAGCAGTGGT3700.1013454290472434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA601.4534053E-615.83949851
GAACAAA1250.015.2059181
AAAAAGT1600.012.4736061
AAAAGTA1701.8189894E-1211.7350412
AAAGTAC2251.8189894E-129.7109023
AAGTACT2304.1836756E-119.086764
AGTACTC2304.1836756E-119.086765
GTGCTGG1000.0028832958.5533291
TACAAAA1000.0028940488.5498152
GGGTAAG1752.414461E-68.142687
ACAAAAA4400.07.9884643
ACCATGT3600.07.65575841
GAAAAAA2656.2482286E-97.5312341
GAAGCAG56450.07.33730849
CAAAAAA5050.07.3364754
GGAAGCA57750.07.15568978
AGTGGTA63000.07.026831614
AACAAAA3258.712959E-107.0152332
CAGTGGT63300.07.008537313
CCATGTA60500.06.971752
Produced by FastQC (version 0.11.5)