##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 365088 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63453742659304 33.0 31.0 34.0 30.0 34.0 2 31.769039245332632 33.0 31.0 34.0 29.0 34.0 3 31.867798996406346 33.0 31.0 34.0 29.0 34.0 4 35.28529012183364 37.0 35.0 37.0 33.0 37.0 5 35.535933802261376 37.0 35.0 37.0 33.0 37.0 6 35.22857228942063 37.0 35.0 37.0 33.0 37.0 7 35.24039957489701 37.0 35.0 37.0 33.0 37.0 8 35.260107152248224 37.0 35.0 37.0 33.0 37.0 9 36.85446248575686 39.0 37.0 39.0 33.0 39.0 10 36.91624211149093 39.0 37.0 39.0 33.0 39.0 11 36.91805537295118 39.0 37.0 39.0 33.0 39.0 12 36.93924204575335 39.0 37.0 39.0 33.0 39.0 13 36.77686201682882 39.0 37.0 39.0 32.0 39.0 14 38.091410837934966 40.0 38.0 41.0 33.0 41.0 15 38.007524213340346 40.0 38.0 41.0 33.0 41.0 16 37.89848200981681 40.0 38.0 41.0 32.0 41.0 17 38.04946752563765 40.0 38.0 41.0 33.0 41.0 18 38.05557016390569 40.0 38.0 41.0 33.0 41.0 19 38.01897624682268 40.0 38.0 41.0 32.0 41.0 20 38.089923525287055 40.0 38.0 41.0 33.0 41.0 21 38.02228503812779 40.0 38.0 41.0 32.0 41.0 22 38.12463570426856 40.0 38.0 41.0 33.0 41.0 23 38.05008655447454 40.0 38.0 41.0 32.0 41.0 24 38.06373805767377 40.0 38.0 41.0 32.0 41.0 25 36.99548054167762 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13280661184643705 1101 2 -0.1193600364462597 1101 3 -0.14553422006375527 1101 4 -0.1544764247774708 1101 5 0.03319322356508536 1101 6 -0.21282318631182306 1101 7 -0.011733111409711228 1101 8 -0.2622604162235973 1101 9 -0.2907956984256188 1101 10 -0.12429321779650593 1101 11 -0.03581066197544658 1101 12 -0.28292704019613524 1101 13 -0.20844844000772156 1101 14 0.03866859607631312 1101 15 -0.0357909851228726 1101 16 -0.20458202419116134 1101 17 -0.0886304086231604 1101 18 -0.23947162073510242 1101 19 -0.1408316623565824 1101 20 -0.05220041600671976 1101 21 -0.041629988009582064 1101 22 -0.31852808467701976 1101 23 -0.24787947365668117 1101 24 -0.13507003268178153 1101 25 0.012644642331565592 1102 1 -0.20690364079296586 1102 2 -0.03310515420475468 1102 3 -0.27848321087227745 1102 4 -0.17396641082264352 1102 5 -0.10401627819291548 1102 6 -0.33338823998724365 1102 7 -0.04488947566975554 1102 8 -0.1871888879748056 1102 9 -0.05249943504574617 1102 10 -0.19583316057390476 1102 11 -0.2632704931142129 1102 12 -0.3804198483912842 1102 13 -0.14208503752391977 1102 14 -0.08754855355221025 1102 15 -0.06548202986408569 1102 16 -0.027316703113129392 1102 17 -0.22112333375822146 1102 18 -0.15282304077548048 1102 19 -0.18454380305869478 1102 20 -0.060015900410832046 1102 21 -0.008064334042593657 1102 22 -0.29519077538652994 1102 23 -0.1058949253176138 1102 24 -0.3090961180791041 1102 25 -0.1039405561934359 1103 1 -0.40521384087263 1103 2 -0.31084207855441903 1103 3 -0.2958254911063669 1103 4 -0.6607605401850236 1103 5 -0.5969384217722862 1103 6 -0.6332626156957986 1103 7 -0.7055430283859963 1103 8 -0.5449653380161692 1103 9 -0.7873096198053702 1103 10 -0.37081860654014065 1103 11 -0.6601283538244473 1103 12 -0.5600932252335156 1103 13 -0.5984076161923042 1103 14 -0.6594071802642247 1103 15 -0.3512696328913236 1103 16 -0.5423073578914526 1103 17 -0.6150397476443032 1103 18 -0.7018701345647429 1103 19 -0.6678373042996171 1103 20 -0.7293482532618896 1103 21 -0.7913547712764029 1103 22 -0.6795321670585608 1103 23 -0.5571772122925225 1103 24 -0.5875607441170345 1103 25 -0.5126867644846413 1104 1 -0.41986560692698305 1104 2 -0.23514757127000863 1104 3 -0.22353942348050992 1104 4 -0.008496793314449747 1104 5 -0.08160044330880112 1104 6 -0.3100188670154367 1104 7 -0.1529007335710446 1104 8 -0.27763658948662595 1104 9 -0.14107850949861245 1104 10 -0.11185260044717893 1104 11 -0.2816030318324678 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2214 25 0.009479505429297319 2215 1 0.41677858471468454 2215 2 0.27994435851286426 2215 3 0.34192230462119255 2215 4 0.29218933850602724 2215 5 0.40013241082135664 2215 6 0.4999642024591253 2215 7 0.39744827287443485 2215 8 0.2752104120282084 2215 9 0.3374019015864249 2215 10 0.4672842397821242 2215 11 0.3260394667566544 2215 12 0.37362474624408293 2215 13 0.29411265127914277 2215 14 0.356454705054432 2215 15 0.38940437148763607 2215 16 0.5235929912416353 2215 17 0.39706451888034167 2215 18 0.42779730137821304 2215 19 0.39735886115396113 2215 20 0.44289270774171996 2215 21 0.5069735321735038 2215 22 0.5201216586116004 2215 23 0.527786395021522 2215 24 0.5572436821803777 2215 25 0.6230757066674997 2216 1 0.06689525783061612 2216 2 -0.040828146499976015 2216 3 -0.12987328877006732 2216 4 0.09608938658238486 2216 5 0.10021801019284737 2216 6 0.035797506477607044 2216 7 0.09872053750547849 2216 8 -0.26227859490182226 2216 9 -0.20357440028620744 2216 10 -0.15856911559598075 2216 11 -0.0778488553784058 2216 12 -0.16969205351201566 2216 13 -0.1984683456603875 2216 14 -0.534777808635603 2216 15 -0.4775601340880513 2216 16 -0.42464401348392045 2216 17 -0.514184176608687 2216 18 -0.35247028460224783 2216 19 -0.658433167551145 2216 20 -0.47390939354696116 2216 21 -0.22093538071447938 2216 22 -0.34072425079377666 2216 23 -0.3839196121782109 2216 24 -0.2376250459067677 2216 25 -0.5712817703789668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 52.0 10 117.0 11 137.0 12 220.0 13 312.0 14 543.0 15 911.0 16 1304.0 17 1577.0 18 1656.0 19 1695.0 20 1727.0 21 1669.0 22 1567.0 23 1554.0 24 1742.0 25 1664.0 26 1669.0 27 1839.0 28 2001.0 29 2333.0 30 3192.0 31 4177.0 32 5668.0 33 7931.0 34 11413.0 35 17707.0 36 29609.0 37 59487.0 38 199603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55192616504349 19.0805158362609 12.07600528332082 23.29155271537479 2 12.357780596520163 24.628989882663 34.07978258546862 28.93344693534822 3 22.207705054987194 31.002095402440528 27.593574099182383 19.196625443389895 4 13.62794723463932 22.629886493119468 28.91357700061355 34.82858927162766 5 10.742341572442808 39.703852221930056 34.774903584889124 14.778902620738013 6 31.67567449110129 30.165208887476613 18.4715644029771 19.687552218444992 7 20.10035936541327 35.8932640897537 23.63348014725217 20.37289639758086 8 28.395345779647645 32.167860899290034 21.924029275133666 17.51276404592865 9 27.458037514243138 16.420424664738363 20.654198439828207 35.467339381190286 10 19.009937330177927 31.361753878516964 29.765426417740382 19.86288237356473 11 25.767486195109125 27.09812428784293 18.39474318520466 28.739646331843282 12 25.162426593040582 33.03778814970637 27.469541589972827 14.330243667280218 13 31.232196073275485 20.442468665088963 23.5220001753002 24.80333508633535 14 22.283942501533875 28.590367253922345 26.30954728722938 22.8161429573144 15 35.68892979226926 19.96066701726707 23.58718993776843 20.76321325269524 16 16.09748882461215 27.285202471732845 35.970779647646594 20.646529056008415 17 25.294175650801996 29.596426067139976 28.11513936366027 16.994258918397755 18 25.280754229117363 21.544942589183975 32.77237268822859 20.401930493470065 19 22.142880620562714 30.32638706284512 33.882242089578405 13.64849022701376 20 23.450236655272153 23.68497458147077 31.53595845385222 21.328830309404857 21 17.831591287579982 28.355629327723726 34.70560522394601 19.107174160750283 22 21.71394293978438 26.953227714961876 24.859759838723814 26.473069506529935 23 16.369204137084758 26.861195109124374 32.5516039968446 24.21799675694627 24 20.75253089666053 34.22489920238409 27.192074239635378 17.83049566132001 25 21.091627224121307 22.938579191866072 25.76392540976422 30.2058681742484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2247.0 1 2247.0 2 1891.5 3 1536.0 4 1536.0 5 1536.0 6 2837.0 7 4138.0 8 4138.0 9 4138.0 10 3815.0 11 3492.0 12 3492.0 13 3492.0 14 2807.0 15 2122.0 16 2122.0 17 2122.0 18 3238.5 19 4355.0 20 4355.0 21 4355.0 22 6500.0 23 8645.0 24 8645.0 25 8645.0 26 11481.0 27 14317.0 28 14317.0 29 14317.0 30 15795.0 31 17273.0 32 17273.0 33 17273.0 34 17834.0 35 18395.0 36 18395.0 37 18395.0 38 17455.5 39 16516.0 40 16516.0 41 16516.0 42 20407.5 43 24299.0 44 24299.0 45 24299.0 46 69637.5 47 114976.0 48 114976.0 49 114976.0 50 97158.5 51 79341.0 52 79341.0 53 79341.0 54 53646.0 55 27951.0 56 27951.0 57 27951.0 58 19145.0 59 10339.0 60 10339.0 61 10339.0 62 8789.5 63 7240.0 64 7240.0 65 7240.0 66 5682.0 67 4124.0 68 4124.0 69 4124.0 70 3163.0 71 2202.0 72 2202.0 73 2202.0 74 1598.0 75 994.0 76 994.0 77 994.0 78 703.5 79 413.0 80 413.0 81 413.0 82 267.5 83 122.0 84 122.0 85 122.0 86 78.5 87 35.0 88 35.0 89 35.0 90 22.0 91 9.0 92 9.0 93 9.0 94 8.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04546848978876326 2 0.01944736611447103 3 8.217196949776493E-4 4 0.0 5 0.0 6 0.00958672977473924 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 365088.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.494240756674117 #Duplication Level Percentage of deduplicated Percentage of total 1 83.86916401569128 23.897881515240964 2 9.537293537942537 5.435158764744139 3 2.8832709752565266 2.4646985200707014 4 1.2520341650107394 1.4270305173358981 5 0.6777124430246423 0.9655450757668972 6 0.3722755617489646 0.6364625690580659 7 0.23753622892469542 0.47378901467889106 8 0.16268222883012531 0.37084052760943526 9 0.11277969045180461 0.28922144869772115 >10 0.6846625318947315 3.7672728155949664 >50 0.07984402700433524 1.6110515962043492 >100 0.07086157396634751 4.527154520737341 >500 0.018962956413529616 3.7672728146370718 >1k 0.02894345978907152 19.892861280581503 >5k 0.009980503375541905 18.741378859922825 >10k+ 0.001996100675108381 11.732380159119227 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 21065 5.76984179156806 No Hit GTACATGGGAAGCAGTGGTATCAAC 20190 5.530173547199579 No Hit GAGTACATGGGAAGCAGTGGTATCA 9780 2.6788062056271364 No Hit CATGTACTCTGCGTTGATACCACTG 7860 2.152905600841441 No Hit GCGTTGATACCACTGCTTCCCATGT 6830 1.8707818388991146 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6810 1.8653037075992636 No Hit GTATCAACGCAGAGTACTTTTTTTT 6527 1.7877881497063721 No Hit TATCAACGCAGAGTACATGGGAAGC 5956 1.6313875010956262 No Hit GTATCAACGCAGAGTACATGGGAAG 5787 1.5850972916118853 No Hit GGTATCAACGCAGAGTACATGGGAA 5706 1.5629108598474888 No Hit ACTCTGCGTTGATACCACTGCTTCC 5439 1.489777806994478 No Hit GGTATCAACGCAGAGTACTTTTTTT 5206 1.4259575773512139 No Hit TATCAACGCAGAGTACTTTTTTTTT 4609 1.2624353580506618 No Hit GCTTCCCATGTACTCTGCGTTGATA 3936 1.0780962398106757 No Hit GTGGTATCAACGCAGAGTACATGGG 3559 0.9748334648084845 No Hit GTACTCTGCGTTGATACCACTGCTT 3311 0.9069046366903323 No Hit GATACCACTGCTTCCCATGTACTCT 2982 0.8167893768077833 No Hit ATACCACTGCTTCCCATGTACTCTG 2969 0.8132285914628801 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2927 0.8017245157331931 No Hit CATGGGAAGCAGTGGTATCAACGCA 2842 0.7784424577088264 No Hit CAGTGGTATCAACGCAGAGTACATG 2791 0.7644732228942064 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2722 0.7455736699097204 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2685 0.735439127004996 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2552 0.699009553860987 No Hit ACATGGGAAGCAGTGGTATCAACGC 2460 0.6738101498816724 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2446 0.6699754579717766 No Hit GCAGTGGTATCAACGCAGAGTACAT 2346 0.6425848014725217 No Hit TACCACTGCTTCCCATGTACTCTGC 2313 0.6335458848277676 No Hit GTTGATACCACTGCTTCCCATGTAC 2093 0.5732864405294067 No Hit CTTCCCATGTACTCTGCGTTGATAC 2075 0.5683561223595407 No Hit CCACTGCTTCCCATGTACTCTGCGT 2074 0.5680822157945482 No Hit CCATGTACTCTGCGTTGATACCACT 2006 0.5494565693750548 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1910 0.52316153913577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1880 0.5149443421859935 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1871 0.5124791831010606 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1768 0.48426680690682794 No Hit TTCCCATGTACTCTGCGTTGATACC 1511 0.4138728197037427 No Hit TGATACCACTGCTTCCCATGTACTC 1503 0.4116815671838023 No Hit ATCAACGCAGAGTACATGGGAAGCA 1425 0.3903168551143834 No Hit GTACATGGTAAGCAGTGGTATCAAC 1269 0.3475874309755456 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1115 0.30540581996669297 No Hit ACCATGTACTCTGCGTTGATACCAC 999 0.2736326584275572 No Hit GGAAGCAGTGGTATCAACGCAGAGT 990 0.27116749934262424 No Hit GTGGTATCAACGCAGAGTACTTTTT 943 0.2582938907879744 No Hit ATCAACGCAGAGTACTTTTTTTTTT 779 0.21337321412919627 No Hit TGGGAAGCAGTGGTATCAACGCAGA 778 0.21309930756420373 No Hit TCAACGCAGAGTACATGGGAAGCAG 775 0.21227758786922604 No Hit AAAAAGTACTCTGCGTTGATACCAC 756 0.2070733631343676 No Hit CCCCATGTACTCTGCGTTGATACCA 749 0.20515601717941973 No Hit CTGCGTTGATACCACTGCTTCCCAT 744 0.203786484354457 No Hit CTCTGCGTTGATACCACTGCTTCCC 711 0.19474756770970286 No Hit TCCATGTACTCTGCGTTGATACCAC 633 0.17338285564028397 No Hit GGTAAGCAGTGGTATCAACGCAGAG 625 0.1711916031203436 No Hit TCCCATGTACTCTGCGTTGATACCA 579 0.1585919011306863 No Hit AAAGTACTCTGCGTTGATACCACTG 567 0.1553050223507757 No Hit AACGCAGAGTACATGGGAAGCAGTG 556 0.15229205013585764 No Hit GTACATGGAAAGCAGTGGTATCAAC 524 0.14352704005609607 No Hit AGTGGTATCAACGCAGAGTACATGG 523 0.14325313349110352 No Hit GTACATGGGTAAGCAGTGGTATCAA 515 0.1410618809711631 No Hit ATGTACTCTGCGTTGATACCACTGC 501 0.1372271890612674 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 500 0.13695328249627486 No Hit GAGTACATGGTAAGCAGTGGTATCA 488 0.1336664037163643 No Hit ACTGCTTCCCATGTACTCTGCGTTG 434 0.11887544920676658 No Hit GCTTACCATGTACTCTGCGTTGATA 431 0.11805372951178895 No Hit CAACGCAGAGTACATGGGAAGCAGT 427 0.11695810325181874 No Hit TTGATACCACTGCTTCCCATGTACT 399 0.10928871943202735 No Hit ACTCTGCGTTGATACCACTGCTTAC 373 0.10216714874222105 No Hit CGCAGAGTACATGGGAAGCAGTGGT 370 0.1013454290472434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 8.217196949776493E-4 0.0 11 0.0 0.0 0.0 8.217196949776493E-4 0.0 12 0.0 0.0 0.0 8.217196949776493E-4 0.0 13 0.0 0.0 0.0 8.217196949776493E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 60 1.4534053E-6 15.8394985 1 GAACAAA 125 0.0 15.205918 1 AAAAAGT 160 0.0 12.473606 1 AAAAGTA 170 1.8189894E-12 11.735041 2 AAAGTAC 225 1.8189894E-12 9.710902 3 AAGTACT 230 4.1836756E-11 9.08676 4 AGTACTC 230 4.1836756E-11 9.08676 5 GTGCTGG 100 0.002883295 8.553329 1 TACAAAA 100 0.002894048 8.549815 2 GGGTAAG 175 2.414461E-6 8.14268 7 ACAAAAA 440 0.0 7.988464 3 ACCATGT 360 0.0 7.6557584 1 GAAAAAA 265 6.2482286E-9 7.531234 1 GAAGCAG 5645 0.0 7.3373084 9 CAAAAAA 505 0.0 7.336475 4 GGAAGCA 5775 0.0 7.1556897 8 AGTGGTA 6300 0.0 7.0268316 14 AACAAAA 325 8.712959E-10 7.015233 2 CAGTGGT 6330 0.0 7.0085373 13 CCATGTA 6050 0.0 6.97175 2 >>END_MODULE