FastQCFastQC Report
Wed 25 May 2016
SRR1294890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153846
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC56760.17997074048637757No Hit
GTATCAACGCAGAGTACTTTTTTTT52680.16703415448947095No Hit
CCCATGTACTCTGCGTTGATACCAC52590.16674878862189213No Hit
GGTATCAACGCAGAGTACTTTTTTT34540.10951707851302821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC750.002648734510.13339216
TTAGTAC1901.0513759E-99.500963
TAGACAG3300.09.4996035
TGTACCG950.00182523528.9996235
AACACCG950.00182523528.9996235
AGACTAT3000.08.8653126
GTAGGAC1304.366097E-58.7701173
AAGGGCG1107.150438E-48.6360035
CCCCGCG1107.1594736E-48.63490712
GTATAGT4150.08.4804261
TGGACTG4150.08.4695265
TAGTACC2252.4447218E-98.4452984
GTCTAGC1701.5842706E-68.3927911
CTAGTAC1701.6104423E-68.3832013
GTCTTAA3750.08.370411
GTGTATA4100.08.351851
TGCGATG1150.0011127248.2596078
GTGCGCA1150.00111289888.2594769
TTAGGCT2451.3951649E-98.1436814
AGACCGT1050.00452074048.1416126
Produced by FastQC (version 0.11.5)