FastQCFastQC Report
Wed 25 May 2016
SRR1294890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153846
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125980.3994487999731122No Hit
GGTATCAACGCAGAGTACTTTTTTT83920.26608781785794233No Hit
TATCAACGCAGAGTACTTTTTTTTT72070.22851464529339732No Hit
CCCATGTACTCTGCGTTGATACCAC58190.18450488704901888No Hit
GTACATGGGAAGCAGTGGTATCAAC56930.18050976490291537No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37620.11928293264794794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG855.3248903E-511.1768355
ACCCGTG750.002650239510.13277
TGGACCG900.0011138519.5003095
ACACCGT900.0011138519.5003096
GAACCGT900.0011138519.5003096
ACACCGG950.00182400189.0002936
TAGGACC1804.220601E-88.9725144
GTATTAG4200.08.8257731
GGTATCA39100.08.775421
GTCTTAA3450.08.5404371
TGCACCG1451.6894826E-58.5175195
GTATTAA5600.08.48632051
TAGACTG2701.4551915E-118.4447195
GTCTAAG2409.240466E-108.3165941
AAGACCG1150.00111068358.2611395
ACAGTCC4150.08.2404488
TAATACT5100.08.1963444
GTCCTAG3500.08.1468681
TATTCCG1050.00451320228.1431225
GACAGTG5650.08.0702917
Produced by FastQC (version 0.11.5)