FastQCFastQC Report
Wed 25 May 2016
SRR1294889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740824
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89650.32709141484458687No Hit
CCCATGTACTCTGCGTTGATACCAC83210.303594831335394No Hit
GAGTACATGGGAAGCAGTGGTATCA36040.13149330274399232No Hit
CATGTACTCTGCGTTGATACCACTG33190.1210949699798309No Hit
GCGTTGATACCACTGCTTCCCATGT28050.10234148562622045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG250.0060316219.0008775
TCGTTAA250.006038620618.9970613
TATGCGC600.005885169411.08141610
CCGGACT700.00149100310.858044
GTATTAG3750.010.6546941
GCGCAAT909.524595E-510.555078516
TAGGACC2250.010.1341714
CTAATAC3650.09.6309343
GTATAGG2900.09.513121
GTATAAC1901.02591E-99.513121
AGTACGG800.0045142269.5007863
TTAGGCT2002.6375346E-109.5007854
CTAGGAC3600.09.5007853
TGTACCG900.00111361439.5004385
CTTAGAC2950.09.3397543
CTAGACA2800.09.1614714
TAGTACA2501.8189894E-129.1207534
ACTGATC2950.09.0152248
TATCCCG950.0018236169.0004165
GTATTGG2750.08.9942221
Produced by FastQC (version 0.11.5)