FastQCFastQC Report
Wed 25 May 2016
SRR1294889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740824
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC91780.3348628003841181No Hit
GTACATGGGAAGCAGTGGTATCAAC82020.29925307133913015No Hit
GAGTACATGGGAAGCAGTGGTATCA36470.13306217400314652No Hit
CATGTACTCTGCGTTGATACCACTG35200.1284285309819237No Hit
GCGTTGATACCACTGCTTCCCATGT29270.10679270175684392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG456.768504E-414.7766719
GTGCGAC551.962258E-413.81714611
TCGTCGC550.00307119612.09000310
ACCGACC600.00588087611.0825038
AGAACCG951.3608875E-511.0001795
TAGACTG3250.010.5232495
CCGTCCC1002.4021923E-510.4492189
CTTACAC3100.010.4195253
GTCTAGG2750.010.024931
GTATTAC2750.09.6792431
TTTATAC3150.09.6509513
GTCCGGT900.00110724469.5063991
TAGGGCC1304.24025E-69.5001554
TCTATAC2106.730261E-119.5001553
ATAGGGC2002.6557245E-109.50015453
GTTAGAC1106.838585E-59.50015453
TTATACT3450.09.3624724
ATTATAC3150.09.3493593
GTGTTAG3950.09.1453961
GTTAAAC2201.6734703E-109.068333
Produced by FastQC (version 0.11.5)