FastQCFastQC Report
Wed 25 May 2016
SRR1294888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310899
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA211976.817969822997179No Hit
GTACATGGGAAGCAGTGGTATCAAC189316.08911575785062No Hit
CCCATGTACTCTGCGTTGATACCAC182835.880687940456547No Hit
GAGTACATGGGAAGCAGTGGTATCA87872.826319801607596No Hit
CATGTACTCTGCGTTGATACCACTG73412.3612169868671176No Hit
GCGTTGATACCACTGCTTCCCATGT58981.8970791157256857No Hit
ACGCAGAGTACATGGGAAGCAGTGG54391.7494427450715506No Hit
TATCAACGCAGAGTACATGGGAAGC51191.6465154278399095No Hit
GTATCAACGCAGAGTACATGGGAAG49611.5956950649567865No Hit
GGTATCAACGCAGAGTACATGGGAA46621.4995223529184718No Hit
ACTCTGCGTTGATACCACTGCTTCC45051.4490236379016979No Hit
GCTTCCCATGTACTCTGCGTTGATA32421.0427823827030642No Hit
GTGGTATCAACGCAGAGTACATGGG31991.0289515244500624No Hit
GTACTCTGCGTTGATACCACTGCTT28900.9295623337482591No Hit
ATACCACTGCTTCCCATGTACTCTG26560.8542967330226214No Hit
GATACCACTGCTTCCCATGTACTCT25880.8324246781108978No Hit
GGGAAGCAGTGGTATCAACGCAGAG23900.7687384005738198No Hit
CAGTGGTATCAACGCAGAGTACATG23350.7510477679246315No Hit
CTGCTTCCCATGTACTCTGCGTTGA22140.7121283760964172No Hit
GCAGAGTACATGGGAAGCAGTGGTA21930.7053737709030907No Hit
CATGGGAAGCAGTGGTATCAACGCA21090.6783553501297849No Hit
GCAGTGGTATCAACGCAGAGTACAT20910.6725656885355051No Hit
GTTGATACCACTGCTTCCCATGTAC20470.6584131824161544No Hit
TACCACTGCTTCCCATGTACTCTGC19540.6284999308457088No Hit
ACATGGGAAGCAGTGGTATCAACGC19030.612095889661916No Hit
GTATCAACGCAGAGTACTTTTTTTT19000.6111309460628693No Hit
CCACTGCTTCCCATGTACTCTGCGT17550.564492005442282No Hit
CTTCCCATGTACTCTGCGTTGATAC16700.5371519368026273No Hit
AAGCAGTGGTATCAACGCAGAGTAC15740.5062737416331349No Hit
ATGGGAAGCAGTGGTATCAACGCAG14600.4696058848693627No Hit
GGTATCAACGCAGAGTACTTTTTTT14180.4560966744827098No Hit
CCATGTACTCTGCGTTGATACCACT14130.4544884351509655No Hit
TTCCCATGTACTCTGCGTTGATACC13100.421358704917031No Hit
TGATACCACTGCTTCCCATGTACTC12300.3956268756091207No Hit
TATCAACGCAGAGTACTTTTTTTTT11660.3750414121627924No Hit
ATCAACGCAGAGTACATGGGAAGCA11080.35638583591455747No Hit
AAACAAAAAAAAAAAAAAAAAAAAA9460.30427888156603916No Hit
GTACATGGTAAGCAGTGGTATCAAC9270.29816757210541045No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8900.28626660105050195No Hit
GGAAGCAGTGGTATCAACGCAGAGT7900.2541018144156141No Hit
ACCATGTACTCTGCGTTGATACCAC7200.23158646377119257No Hit
AAAAACAAAAAAAAAAAAAAAAAAA6320.20328145153249125No Hit
CTGCGTTGATACCACTGCTTCCCAT6020.1936320155420249No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5970.19202377621028052No Hit
CTCTGCGTTGATACCACTGCTTCCC5930.190737184744885No Hit
TCAACGCAGAGTACATGGGAAGCAG5630.18108774875441866No Hit
AAAAAGTACTCTGCGTTGATACCAC5610.18044445302172088No Hit
TGGGAAGCAGTGGTATCAACGCAGA5560.1788362136899765No Hit
CCCCATGTACTCTGCGTTGATACCA5120.16468370757062584No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA5010.16114558104078816No Hit
TCCATGTACTCTGCGTTGATACCAC4840.15567756731285723No Hit
AAAGTACTCTGCGTTGATACCACTG4670.1502095535849263No Hit
GGTAAGCAGTGGTATCAACGCAGAG4650.14956625785222855No Hit
GTACATGGGTAAGCAGTGGTATCAA4350.1399168218617622No Hit
GTACATGGAAAGCAGTGGTATCAAC4290.1379869346636689No Hit
ACTGCTTCCCATGTACTCTGCGTTG4230.13605704746557562No Hit
TCCCATGTACTCTGCGTTGATACCA4200.135092103866529No Hit
AGTGGTATCAACGCAGAGTACATGG4120.13251892093573797No Hit
ACACAAAAAAAAAAAAAAAAAAAAA4110.13219727306938908No Hit
GAGTACATGGTAAGCAGTGGTATCA4070.13091068160399358No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3980.12801585080685365No Hit
AACGCAGAGTACATGGGAAGCAGTG3920.1260859636087604No Hit
ATGTACTCTGCGTTGATACCACTGC3670.11804476695003845No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3560.11450664042020077No Hit
TTGATACCACTGCTTCCCATGTACT3440.11064686602401423No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA3170.1019623736325945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAC350.002145150416.3081111
GTACAAA601.4368525E-615.8551081
CGCAAAA400.00526355914.2512072
AGAACAA759.591968E-713.9345142
GCAAAAA1053.4330606E-610.8580643
AGTACTC1002.7365854E-49.5008063
TACAAAA1752.6213456E-89.2293542
GAGAACA1252.6867887E-59.1325421
ACCCATG850.0074213188.93905457
GAACAAA1502.621582E-68.87885951
AAGTACT1107.095946E-48.6370952
ACAACAA1100.0068157567.7733865
CCATGTA51200.07.70084762
CATGGTA2251.8198443E-77.6006444
GAAGCAG48650.07.5162029
GGAAGCA49300.07.4363698
TGCGTTG53800.07.432250512
CTGCGTT54200.07.359876611
TCTGCGT55000.07.270092510
TTGATAC55150.07.251487316
Produced by FastQC (version 0.11.5)