##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294888_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310899 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.360432166073227 31.0 30.0 34.0 16.0 34.0 2 29.692440310197203 31.0 30.0 34.0 20.0 34.0 3 30.074628737950267 31.0 30.0 34.0 23.0 34.0 4 33.04472513581581 37.0 35.0 37.0 20.0 37.0 5 33.91493378878671 37.0 35.0 37.0 28.0 37.0 6 33.698905432310816 37.0 35.0 37.0 26.0 37.0 7 34.0347894332243 37.0 35.0 37.0 28.0 37.0 8 34.033013937002046 37.0 35.0 37.0 27.0 37.0 9 35.21513417540745 39.0 35.0 39.0 26.0 39.0 10 35.513037996262454 39.0 35.0 39.0 27.0 39.0 11 35.441329820938634 39.0 35.0 39.0 27.0 39.0 12 35.65778275259811 39.0 35.0 39.0 28.0 39.0 13 35.20932521494119 39.0 35.0 39.0 26.0 39.0 14 36.447663067427044 40.0 36.0 41.0 27.0 41.0 15 36.35718030614444 40.0 36.0 41.0 26.0 41.0 16 36.247298962042336 40.0 36.0 41.0 27.0 41.0 17 36.26314011946002 40.0 36.0 41.0 27.0 41.0 18 36.09141232361635 40.0 36.0 41.0 26.0 41.0 19 35.953280647412825 40.0 35.0 41.0 26.0 41.0 20 36.00165327003303 40.0 35.0 41.0 26.0 41.0 21 35.889980347315365 40.0 35.0 41.0 25.0 41.0 22 35.962315735978564 40.0 35.0 41.0 26.0 41.0 23 35.93270161692382 40.0 35.0 41.0 25.0 41.0 24 36.046745084416486 40.0 35.0 41.0 25.0 41.0 25 35.05398216140933 39.0 33.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3961848905967926 1101 2 0.28066908926365386 1101 3 0.17838114028462826 1101 4 0.42990746878986386 1101 5 0.15836250278442776 1101 6 0.3149356560234793 1101 7 0.07268569291102267 1101 8 0.12912192664508382 1101 9 0.5718513462532897 1101 10 0.1534749908759494 1101 11 0.33474701979596233 1101 12 0.2836085434867215 1101 13 0.25000269150616816 1101 14 0.433350342373231 1101 15 0.5195083757037651 1101 16 0.2838807379979187 1101 17 0.1826836526991471 1101 18 -0.23184033514886693 1101 19 -0.0856040748864757 1101 20 0.15938096740865149 1101 21 -0.09172144555456185 1101 22 0.04055567882686262 1101 23 0.056849692396497176 1101 24 0.009794783282565334 1101 25 -0.23724905950680863 1102 1 0.1862071609620557 1102 2 0.05878353167127415 1102 3 0.22327031870510083 1102 4 -0.10124326774818115 1102 5 0.1205533815296107 1102 6 0.41452105353182844 1102 7 0.3943788466674363 1102 8 0.3675966338731058 1102 9 0.4955008581737417 1102 10 0.40342827865855924 1102 11 0.6352909015054777 1102 12 0.41427145297611645 1102 13 0.7078076717860355 1102 14 0.8924935645567373 1102 15 0.7940348148188079 1102 16 0.8170868121011665 1102 17 0.690170232405336 1102 18 0.9904845697854583 1102 19 0.4980764438463865 1102 20 0.5243675092400721 1102 21 0.4008172245864827 1102 22 0.5010522928223509 1102 23 0.6864445333477107 1102 24 0.2925274227500978 1102 25 0.3435075269158432 1103 1 -0.10266181843161704 1103 2 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0.5671950230891554 2210 23 0.722663680000565 2210 24 0.7852697163518485 2210 25 0.6304344040205265 2211 1 -0.09526042559566505 2211 2 0.35851873748857344 2211 3 0.10579799037848048 2211 4 0.2583726671387936 2211 5 0.2204711727010249 2211 6 0.07659734877506708 2211 7 0.11418299889457018 2211 8 0.14913462025641167 2211 9 0.3470844000570992 2211 10 0.336339515833302 2211 11 0.058566446422844365 2211 12 0.2905585207552548 2211 13 0.1461896566977643 2211 14 0.5309246022501668 2211 15 0.36017204451754026 2211 16 0.1598794596342259 2211 17 0.05450068464473645 2211 18 0.19547523613116624 2211 19 0.3638307893323969 2211 20 0.15814761652032416 2211 21 -0.023175944359472567 2211 22 0.12305064864577275 2211 23 0.09934178139582173 2211 24 0.16445878519110835 2211 25 0.25180607667943633 2212 1 0.3842861767274357 2212 2 0.302701763934099 2212 3 0.43698431149241657 2212 4 0.46301887105552453 2212 5 0.5937306498828363 2212 6 0.4750959098627092 2212 7 0.4073203088098438 2212 8 0.617450491579973 2212 9 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0.40946994442715834 2213 21 0.5736359878397792 2213 22 0.6144193151904958 2213 23 0.4087665115951964 2213 24 0.416851931950454 2213 25 0.3416862669117364 2214 1 -0.021195345459677384 2214 2 0.11119957346226883 2214 3 0.07950999420219063 2214 4 0.060407095933790345 2214 5 0.2623893484220119 2214 6 0.10577625362177656 2214 7 0.0256286845024718 2214 8 0.2209216643601195 2214 9 -0.025014091274030648 2214 10 0.151224997304503 2214 11 -0.0635146994562632 2214 12 -0.09152324185103566 2214 13 -0.39538546452767065 2214 14 -0.04702971368375586 2214 15 -0.1065169785095108 2214 16 -0.04263832909045817 2214 17 -0.02432861298009925 2214 18 0.3094695468229034 2214 19 -0.03952420654324129 2214 20 -0.08567547528722486 2214 21 -0.22179678819644266 2214 22 -0.05906117941068345 2214 23 -0.39395901957860957 2214 24 -0.020277987938086994 2214 25 -0.3108302778461862 2215 1 -0.013001735842468776 2215 2 0.19119184032577863 2215 3 0.29268444673127547 2215 4 0.16264970551584668 2215 5 0.5048969936819532 2215 6 0.4381464599559166 2215 7 0.051590932736154116 2215 8 0.045112321325213145 2215 9 -0.03884586040475568 2215 10 -0.09765897049752681 2215 11 -0.05966219145950191 2215 12 -0.045106847601680045 2215 13 -0.2147429111866046 2215 14 0.08415591674262402 2215 15 0.1588245097677543 2215 16 0.19369335216371297 2215 17 0.11937063831168615 2215 18 -0.06579144653363045 2215 19 0.13370508095368194 2215 20 0.10638751462454366 2215 21 0.06925386384756393 2215 22 0.4203721925883315 2215 23 0.341606378150658 2215 24 0.3416503750975224 2215 25 0.11276633363400634 2216 1 -0.20230777297105718 2216 2 -0.45924862618715423 2216 3 -0.0738367802707316 2216 4 -0.5087108466018719 2216 5 -0.1719084213187969 2216 6 -0.5875648395447257 2216 7 -0.6334929300970984 2216 8 -0.2833081375562827 2216 9 -0.8028022616855139 2216 10 -0.6753254104331532 2216 11 -0.24976455222729044 2216 12 -0.20629602423621662 2216 13 -0.40623883832299157 2216 14 -0.373361876113691 2216 15 -0.6591591324174857 2216 16 -0.4748402510348555 2216 17 -0.5535526989479607 2216 18 -0.11698178447347374 2216 19 -0.2982938572814646 2216 20 0.025096143122979697 2216 21 -0.04227789785258551 2216 22 0.04778895188292154 2216 23 0.024971509129308345 2216 24 0.16234483567120606 2216 25 -0.08859073456779498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116.0 3 73.0 4 7.0 5 11.0 6 20.0 7 33.0 8 24.0 9 79.0 10 182.0 11 323.0 12 564.0 13 1028.0 14 1720.0 15 2712.0 16 3516.0 17 3845.0 18 3821.0 19 3577.0 20 3457.0 21 3158.0 22 2928.0 23 2920.0 24 2840.0 25 2804.0 26 2810.0 27 2835.0 28 2855.0 29 3122.0 30 3532.0 31 4201.0 32 5557.0 33 7419.0 34 9983.0 35 14753.0 36 25335.0 37 54045.0 38 134694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49495219478893 25.784865279150875 9.949731266765637 21.770451259294564 2 10.924480675861892 32.24606884827879 29.96722430424088 26.862226171618435 3 20.93181196388625 38.70576307192172 22.840497610119577 17.521927354072453 4 13.020994332818134 30.7518675940237 23.82244976816074 32.40468830499743 5 9.412446476288594 47.470139403110004 30.02800940085638 13.089404719745017 6 28.66344968339863 36.8970168328274 16.461339866019838 17.978193617754144 7 18.655700236604485 42.819939158846914 19.923063303771187 18.601297300777414 8 25.415691488505505 40.57889365702273 18.78008028922148 15.225334565250284 9 25.297946726932064 26.87218713179193 16.236889378223328 31.592976763052675 10 16.513380483455073 40.49974407592092 25.721818573971717 17.265056866652287 11 22.708206579298935 37.00670548961663 14.222205053421796 26.06288287766264 12 22.72822388266313 41.41912969574441 22.667379203997076 13.185267217595378 13 27.80253034156392 30.306795866464924 18.02562534204681 23.86504844992435 14 19.448056964796166 38.45349076768561 20.747276660400196 21.351175607118027 15 33.41895941774263 29.034646373928467 18.220329435093173 19.326064773235732 16 14.617799024578684 36.04551966133887 30.5123376309817 18.824343683100743 17 24.314440058978438 38.11030770518508 22.284641783260682 15.2906104525758 18 24.576732266860688 30.66328846593415 26.144569392084836 18.615409875120324 19 20.836740343941745 39.655028103813486 26.707314717641985 12.800916834602779 20 22.816235725163313 32.8951477628711 23.793057929999776 20.495558581965813 21 17.196917014371813 36.71444392932571 27.859230927728838 18.22940812857364 22 20.697148817802503 35.452724978107454 17.174380569721322 26.67574563436872 23 15.341000560231304 35.86575054896228 25.114461051045446 23.678787839760968 24 20.077601751674397 42.93437660999484 19.694744976816075 17.293276661514685 25 20.501114015995466 30.93551586023929 18.20482439759424 30.358545726171005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21999.0 1 21999.0 2 14097.5 3 6196.0 4 6196.0 5 6196.0 6 5592.0 7 4988.0 8 4988.0 9 4988.0 10 4525.0 11 4062.0 12 4062.0 13 4062.0 14 3788.5 15 3515.0 16 3515.0 17 3515.0 18 3804.5 19 4094.0 20 4094.0 21 4094.0 22 4858.0 23 5622.0 24 5622.0 25 5622.0 26 6569.5 27 7517.0 28 7517.0 29 7517.0 30 8321.0 31 9125.0 32 9125.0 33 9125.0 34 9395.0 35 9665.0 36 9665.0 37 9665.0 38 9567.0 39 9469.0 40 9469.0 41 9469.0 42 13234.5 43 17000.0 44 17000.0 45 17000.0 46 57214.0 47 97428.0 48 97428.0 49 97428.0 50 81496.5 51 65565.0 52 65565.0 53 65565.0 54 44480.0 55 23395.0 56 23395.0 57 23395.0 58 15986.5 59 8578.0 60 8578.0 61 8578.0 62 7425.0 63 6272.0 64 6272.0 65 6272.0 66 4879.0 67 3486.0 68 3486.0 69 3486.0 70 2627.0 71 1768.0 72 1768.0 73 1768.0 74 1264.0 75 760.0 76 760.0 77 760.0 78 526.0 79 292.0 80 292.0 81 292.0 82 185.0 83 78.0 84 78.0 85 78.0 86 48.5 87 19.0 88 19.0 89 19.0 90 12.0 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11836641481638732 2 0.09745930350371021 3 0.06883264339866002 4 0.10903862669226984 5 0.0929562333748259 6 0.08234185378531292 7 0.08169855805261515 8 0.08748821964689497 9 0.08780986751324385 10 0.08362844525070842 11 0.08298514951801067 12 0.08748821964689497 13 0.08652327604784833 14 0.08073361445356853 15 0.07912537512182413 16 0.08491503671610394 17 0.08909645897863937 18 0.09006140257768601 19 0.08523668458245283 20 0.09649435990466358 21 0.09359952910752366 22 0.09359952910752366 23 0.10067578216719898 24 0.10903862669226984 25 0.10003248643450123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 310899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.278624247746052 #Duplication Level Percentage of deduplicated Percentage of total 1 77.99342889695556 18.93573153982483 2 11.15895180307888 5.418479956513209 3 4.2977133621260695 3.1302770353072864 4 2.2283458307940966 2.164046844795255 5 1.1698153202087915 1.4200753299302988 6 0.6783074110383933 0.988102245423755 7 0.48223417503510774 0.8195587634569426 8 0.2742375665721629 0.5326488666737429 9 0.24376672584192258 0.5326488666737429 >10 1.175114596857529 5.327775258202825 >50 0.13248191621843616 2.2843431468097357 >100 0.09936143716382714 5.590561565009859 >500 0.018547468270581065 3.0202734650159697 >1k 0.03709493654116213 20.56712951794634 >5k 0.006624095810921809 10.480574077111859 >10k+ 0.003974457486553085 18.787773521304345 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 21197 6.817969822997179 No Hit GTACATGGGAAGCAGTGGTATCAAC 18931 6.08911575785062 No Hit CCCATGTACTCTGCGTTGATACCAC 18283 5.880687940456547 No Hit GAGTACATGGGAAGCAGTGGTATCA 8787 2.826319801607596 No Hit CATGTACTCTGCGTTGATACCACTG 7341 2.3612169868671176 No Hit GCGTTGATACCACTGCTTCCCATGT 5898 1.8970791157256857 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5439 1.7494427450715506 No Hit TATCAACGCAGAGTACATGGGAAGC 5119 1.6465154278399095 No Hit GTATCAACGCAGAGTACATGGGAAG 4961 1.5956950649567865 No Hit GGTATCAACGCAGAGTACATGGGAA 4662 1.4995223529184718 No Hit ACTCTGCGTTGATACCACTGCTTCC 4505 1.4490236379016979 No Hit GCTTCCCATGTACTCTGCGTTGATA 3242 1.0427823827030642 No Hit GTGGTATCAACGCAGAGTACATGGG 3199 1.0289515244500624 No Hit GTACTCTGCGTTGATACCACTGCTT 2890 0.9295623337482591 No Hit ATACCACTGCTTCCCATGTACTCTG 2656 0.8542967330226214 No Hit GATACCACTGCTTCCCATGTACTCT 2588 0.8324246781108978 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2390 0.7687384005738198 No Hit CAGTGGTATCAACGCAGAGTACATG 2335 0.7510477679246315 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2214 0.7121283760964172 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2193 0.7053737709030907 No Hit CATGGGAAGCAGTGGTATCAACGCA 2109 0.6783553501297849 No Hit GCAGTGGTATCAACGCAGAGTACAT 2091 0.6725656885355051 No Hit GTTGATACCACTGCTTCCCATGTAC 2047 0.6584131824161544 No Hit TACCACTGCTTCCCATGTACTCTGC 1954 0.6284999308457088 No Hit ACATGGGAAGCAGTGGTATCAACGC 1903 0.612095889661916 No Hit GTATCAACGCAGAGTACTTTTTTTT 1900 0.6111309460628693 No Hit CCACTGCTTCCCATGTACTCTGCGT 1755 0.564492005442282 No Hit CTTCCCATGTACTCTGCGTTGATAC 1670 0.5371519368026273 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1574 0.5062737416331349 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1460 0.4696058848693627 No Hit GGTATCAACGCAGAGTACTTTTTTT 1418 0.4560966744827098 No Hit CCATGTACTCTGCGTTGATACCACT 1413 0.4544884351509655 No Hit TTCCCATGTACTCTGCGTTGATACC 1310 0.421358704917031 No Hit TGATACCACTGCTTCCCATGTACTC 1230 0.3956268756091207 No Hit TATCAACGCAGAGTACTTTTTTTTT 1166 0.3750414121627924 No Hit ATCAACGCAGAGTACATGGGAAGCA 1108 0.35638583591455747 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 946 0.30427888156603916 No Hit GTACATGGTAAGCAGTGGTATCAAC 927 0.29816757210541045 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 890 0.28626660105050195 No Hit GGAAGCAGTGGTATCAACGCAGAGT 790 0.2541018144156141 No Hit ACCATGTACTCTGCGTTGATACCAC 720 0.23158646377119257 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 632 0.20328145153249125 No Hit CTGCGTTGATACCACTGCTTCCCAT 602 0.1936320155420249 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 597 0.19202377621028052 No Hit CTCTGCGTTGATACCACTGCTTCCC 593 0.190737184744885 No Hit TCAACGCAGAGTACATGGGAAGCAG 563 0.18108774875441866 No Hit AAAAAGTACTCTGCGTTGATACCAC 561 0.18044445302172088 No Hit TGGGAAGCAGTGGTATCAACGCAGA 556 0.1788362136899765 No Hit CCCCATGTACTCTGCGTTGATACCA 512 0.16468370757062584 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 501 0.16114558104078816 No Hit TCCATGTACTCTGCGTTGATACCAC 484 0.15567756731285723 No Hit AAAGTACTCTGCGTTGATACCACTG 467 0.1502095535849263 No Hit GGTAAGCAGTGGTATCAACGCAGAG 465 0.14956625785222855 No Hit GTACATGGGTAAGCAGTGGTATCAA 435 0.1399168218617622 No Hit GTACATGGAAAGCAGTGGTATCAAC 429 0.1379869346636689 No Hit ACTGCTTCCCATGTACTCTGCGTTG 423 0.13605704746557562 No Hit TCCCATGTACTCTGCGTTGATACCA 420 0.135092103866529 No Hit AGTGGTATCAACGCAGAGTACATGG 412 0.13251892093573797 No Hit ACACAAAAAAAAAAAAAAAAAAAAA 411 0.13219727306938908 No Hit GAGTACATGGTAAGCAGTGGTATCA 407 0.13091068160399358 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 398 0.12801585080685365 No Hit AACGCAGAGTACATGGGAAGCAGTG 392 0.1260859636087604 No Hit ATGTACTCTGCGTTGATACCACTGC 367 0.11804476695003845 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 356 0.11450664042020077 No Hit TTGATACCACTGCTTCCCATGTACT 344 0.11064686602401423 No Hit CAAAAAAAAAAAAAAAAAAAAAAAA 317 0.1019623736325945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.216478663488786E-4 0.0 10 0.0 0.0 0.0 0.0012865914653955144 0.0 11 0.0 0.0 0.0 0.0012865914653955144 0.0 12 0.0 0.0 0.0 0.0012865914653955144 0.0 13 0.0 0.0 0.0 0.0012865914653955144 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACAAC 35 0.0021451504 16.308111 1 GTACAAA 60 1.4368525E-6 15.855108 1 CGCAAAA 40 0.005263559 14.251207 2 AGAACAA 75 9.591968E-7 13.934514 2 GCAAAAA 105 3.4330606E-6 10.858064 3 AGTACTC 100 2.7365854E-4 9.500806 3 TACAAAA 175 2.6213456E-8 9.229354 2 GAGAACA 125 2.6867887E-5 9.132542 1 ACCCATG 85 0.007421318 8.9390545 7 GAACAAA 150 2.621582E-6 8.8788595 1 AAGTACT 110 7.095946E-4 8.637095 2 ACAACAA 110 0.006815756 7.773386 5 CCATGTA 5120 0.0 7.7008476 2 CATGGTA 225 1.8198443E-7 7.600644 4 GAAGCAG 4865 0.0 7.516202 9 GGAAGCA 4930 0.0 7.436369 8 TGCGTTG 5380 0.0 7.4322505 12 CTGCGTT 5420 0.0 7.3598766 11 TCTGCGT 5500 0.0 7.2700925 10 TTGATAC 5515 0.0 7.2514873 16 >>END_MODULE