FastQCFastQC Report
Wed 25 May 2016
SRR1294888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310899
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC197856.3638030357125634No Hit
GTACATGGGAAGCAGTGGTATCAAC187656.035722212036706No Hit
GAGTACATGGGAAGCAGTGGTATCA88882.858806236108833No Hit
GTATCAACGCAGAGTACTTTTTTTT81702.627863068070338No Hit
CATGTACTCTGCGTTGATACCACTG76332.4551381638409904No Hit
ACGCAGAGTACATGGGAAGCAGTGG61841.9890704055014652No Hit
GGTATCAACGCAGAGTACTTTTTTT61241.9697715335205324No Hit
GCGTTGATACCACTGCTTCCCATGT59981.9292439023605736No Hit
TATCAACGCAGAGTACTTTTTTTTT57811.859446315362867No Hit
TATCAACGCAGAGTACATGGGAAGC52161.6777152708757508No Hit
ACTCTGCGTTGATACCACTGCTTCC48171.5493777722025481No Hit
GTATCAACGCAGAGTACATGGGAAG47961.5426231670092216No Hit
GGTATCAACGCAGAGTACATGGGAA47621.5316871395533598No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34281.1026088858439558No Hit
GCTTCCCATGTACTCTGCGTTGATA34031.0945676891852338No Hit
GTGGTATCAACGCAGAGTACATGGG33351.0726956342735101No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32991.0611163110849504No Hit
GTACTCTGCGTTGATACCACTGCTT27940.8986841385787668No Hit
ATACCACTGCTTCCCATGTACTCTG27110.8719873656718098No Hit
CAGTGGTATCAACGCAGAGTACATG25190.8102309753328251No Hit
GATACCACTGCTTCCCATGTACTCT25140.8086227360010807No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25130.8083010881347318No Hit
GGGAAGCAGTGGTATCAACGCAGAG24390.7844991460249149No Hit
CATGGGAAGCAGTGGTATCAACGCA24130.776136301499844No Hit
CTGCTTCCCATGTACTCTGCGTTGA23450.7542642465881203No Hit
GCAGAGTACATGGGAAGCAGTGGTA22880.7359303182062342No Hit
GCAGTGGTATCAACGCAGAGTACAT21880.7037655315713464No Hit
TACCACTGCTTCCCATGTACTCTGC20960.6741739278672495No Hit
ACATGGGAAGCAGTGGTATCAACGC20870.6712790970701096No Hit
GTTGATACCACTGCTTCCCATGTAC19810.6371844232371284No Hit
CCACTGCTTCCCATGTACTCTGCGT19510.6275349872466621No Hit
CTTCCCATGTACTCTGCGTTGATAC18980.6104876503301715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17810.5728548499673527No Hit
AAGCAGTGGTATCAACGCAGAGTAC17070.5490529078575357No Hit
ATGGGAAGCAGTGGTATCAACGCAG15610.5020923193705995No Hit
CCATGTACTCTGCGTTGATACCACT15360.4940511227118775No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14360.46188633607698965No Hit
TTCCCATGTACTCTGCGTTGATACC13230.4255401271795664No Hit
TGATACCACTGCTTCCCATGTACTC12680.407849494530378No Hit
ATCAACGCAGAGTACATGGGAAGCA11730.37729294722723455No Hit
GTGGTATCAACGCAGAGTACTTTTT10880.3499528785875799No Hit
ATCAACGCAGAGTACTTTTTTTTTT10160.3267942322104606No Hit
GTACATGGTAAGCAGTGGTATCAAC9760.31392831755650547No Hit
GGAAGCAGTGGTATCAACGCAGAGT8770.2820851787879665No Hit
ACCATGTACTCTGCGTTGATACCAC7920.25474511014831186No Hit
CTGCGTTGATACCACTGCTTCCCAT7190.23126481590484368No Hit
TCAACGCAGAGTACATGGGAAGCAG6430.20681957806232892No Hit
AAAAAGTACTCTGCGTTGATACCAC6120.1968484942055137No Hit
CCCCATGTACTCTGCGTTGATACCA6000.19298871980932716No Hit
TGGGAAGCAGTGGTATCAACGCAGA5790.1862341146160007No Hit
CTCTGCGTTGATACCACTGCTTCCC5760.18526917101695406No Hit
TCCATGTACTCTGCGTTGATACCAC5470.1759413828928366No Hit
GTACATGGAAAGCAGTGGTATCAAC5190.16693524263506798No Hit
AACGCAGAGTACATGGGAAGCAGTG4910.15792910237729937No Hit
GTACATGGGTAAGCAGTGGTATCAA4910.15792910237729937No Hit
AGTGGTATCAACGCAGAGTACATGG4680.15053120145127516No Hit
AACGCAGAGTACTTTTTTTTTTTTT4680.15053120145127516No Hit
AAAGTACTCTGCGTTGATACCACTG4510.14506318772334423No Hit
GGTAAGCAGTGGTATCAACGCAGAG4480.1440982441242976No Hit
ACTGCTTCCCATGTACTCTGCGTTG4260.13702199106462226No Hit
TCCCATGTACTCTGCGTTGATACCA4110.13219727306938908No Hit
CAACGCAGAGTACTTTTTTTTTTTT4060.1305890337376447No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3850.12383442854431825No Hit
GAGTACATGGTAAGCAGTGGTATCA3700.11900971054908506No Hit
ATGTACTCTGCGTTGATACCACTGC3660.11772311908368954No Hit
TTGATACCACTGCTTCCCATGTACT3650.11740147121734068No Hit
TCAACGCAGAGTACTTTTTTTTTTT3590.11547158401924741No Hit
GCTTACCATGTACTCTGCGTTGATA3300.10614379589512994No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA250.00600087719.0112671
GCAAAGA350.00216568616.2848873
ACGCAAA651.8861101E-716.0864561
ACTTAGA456.7105814E-414.786541
TGAACAG400.00526831614.2492775
TAGATGT400.00526831614.2492774
CGCAAAA500.001491795513.3036032
GAACAAA1251.0004442E-1012.9276611
GAATCAA657.995192E-411.69171318
GAGAACA700.001478262610.8635811
AAAAGTA1053.4254517E-610.86008552
AAAAAGT1105.9516206E-610.36978151
CATGGGT2002.582965E-109.4995174
GCAAAAA900.00110876159.49951653
AATCAAC1054.4680826E-49.0471619
ATGGGTA1701.6613194E-78.9407225
GGGTAAG1201.752203E-48.70789057
TGGGTAA1356.233413E-47.74034746
AAAGTAC1356.233413E-47.74034743
GAAGCAG51400.07.4665479
Produced by FastQC (version 0.11.5)