##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294888_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310899 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.520731813225517 33.0 31.0 34.0 28.0 34.0 2 31.646242027153512 33.0 31.0 34.0 28.0 34.0 3 31.71938796843991 33.0 31.0 34.0 28.0 34.0 4 35.135449132998176 37.0 35.0 37.0 32.0 37.0 5 35.39522803225485 37.0 35.0 37.0 33.0 37.0 6 35.084850707142834 37.0 35.0 37.0 32.0 37.0 7 35.07775193873251 37.0 35.0 37.0 32.0 37.0 8 35.101087491436125 37.0 35.0 37.0 32.0 37.0 9 36.65099276613949 39.0 37.0 39.0 32.0 39.0 10 36.7068533510883 39.0 37.0 39.0 32.0 39.0 11 36.72286819835381 39.0 37.0 39.0 32.0 39.0 12 36.735492877108 39.0 37.0 39.0 32.0 39.0 13 36.556984744241696 39.0 37.0 39.0 32.0 39.0 14 37.83445749262622 40.0 38.0 41.0 32.0 41.0 15 37.75161386816941 40.0 38.0 41.0 32.0 41.0 16 37.63618409837278 40.0 38.0 41.0 32.0 41.0 17 37.83167202210364 40.0 38.0 41.0 32.0 41.0 18 37.834508956284836 40.0 38.0 41.0 32.0 41.0 19 37.80217047980212 40.0 38.0 41.0 32.0 41.0 20 37.87106745277405 40.0 38.0 41.0 32.0 41.0 21 37.789622996535854 40.0 38.0 41.0 32.0 41.0 22 37.89156928777513 40.0 38.0 41.0 32.0 41.0 23 37.82059125310792 40.0 38.0 41.0 32.0 41.0 24 37.82855203780006 40.0 38.0 41.0 32.0 41.0 25 36.72826223307248 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1580135846944124 1101 2 -0.04593984251550154 1101 3 -0.13670105339565808 1101 4 -0.3938598091298573 1101 5 0.03636330474999028 1101 6 0.10162992892941247 1101 7 0.10472301751123325 1101 8 0.2770858680987658 1101 9 0.20887847653249025 1101 10 0.3397958966314718 1101 11 0.28784853160933466 1101 12 0.20361917979204947 1101 13 0.12201273264336976 1101 14 0.28882406885426803 1101 15 0.4766146892147418 1101 16 0.18836785640500864 1101 17 0.13999973144102285 1101 18 0.1945487454730639 1101 19 0.21981966770479033 1101 20 0.030530213646720483 1101 21 0.02931752464633064 1101 22 0.16882422294396093 1101 23 0.24212450053315848 1101 24 0.08925031795816807 1101 25 0.09422585909045011 1102 1 -0.515894819512944 1102 2 -0.550643889056019 1102 3 -0.42252427416517335 1102 4 -0.6060746752299195 1102 5 -0.39314669671450275 1102 6 -0.4857774672558435 1102 7 -0.43294217961432224 1102 8 -0.060297540731610866 1102 9 -0.36557574801945236 1102 10 0.06956193154695711 1102 11 -0.14956972201023433 1102 12 -0.3022665595992322 1102 13 -0.2258847125770842 1102 14 -0.07742496533907683 1102 15 -0.02420593082050715 1102 16 -0.06336807059263805 1102 17 -0.13086042985500512 1102 18 -0.06905460972664201 1102 19 0.13007161068053819 1102 20 0.07077847014477356 1102 21 -0.5992723723847959 1102 22 -0.5864542576212344 1102 23 -0.5921203522854839 1102 24 -0.4867138474586312 1102 25 -0.7921447783671951 1103 1 -0.1767688241076968 1103 2 -0.2774611449158364 1103 3 -0.11315407601008332 1103 4 -0.03241593843733881 1103 5 -0.14484970933256136 1103 6 -0.13512954665802113 1103 7 -0.29176105715257705 1103 8 -0.1320918478394688 1103 9 -0.09160218538212206 1103 10 0.06421639892069209 1103 11 0.10331701576336627 1103 12 -0.2551227657726187 1103 13 0.035801132272332836 1103 14 0.27385360798431435 1103 15 0.09937197230922834 1103 16 0.26934128358894327 1103 17 0.25971381448789543 1103 18 -0.12080316948598835 1103 19 0.15719968824081576 1103 20 0.05529744728611519 1103 21 0.1833426341469675 1103 22 0.12800020274524826 1103 23 0.08817875729292979 1103 24 0.14058540727001656 1103 25 0.3706387025355369 1104 1 -0.12092010365145711 1104 2 -0.26350380605701673 1104 3 -0.7361461653990204 1104 4 -0.5930284540503905 1104 5 -0.5460590548916784 1104 6 -0.5471494151695282 1104 7 -0.3564983222306637 1104 8 -0.6317190711859411 1104 9 -0.5786130975714343 1104 10 -0.3234955270274682 1104 11 -0.6851104477093628 1104 12 -0.26953518262406106 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0.11403041916413414 2215 2 -0.16556301842831544 2215 3 -0.001504123814147107 2215 4 -0.003448709649120474 2215 5 0.15872440730935722 2215 6 0.1436963041526127 2215 7 -0.10986557514521422 2215 8 -0.1572428883406971 2215 9 -0.12536202589962642 2215 10 0.0351800068053123 2215 11 -0.14606153139992273 2215 12 -0.22274619179837174 2215 13 -0.23871218166814145 2215 14 -0.25505869698527306 2215 15 -0.3579603574777863 2215 16 -0.3938048341107674 2215 17 -0.34297134997843415 2215 18 -0.3476181982701405 2215 19 -0.3928765948406152 2215 20 -0.277422909108445 2215 21 -0.43600275530359056 2215 22 -0.46986387097472004 2215 23 -0.15516630668314946 2215 24 0.02636420667602124 2215 25 -0.3328865929342655 2216 1 0.2603349797759762 2216 2 0.2523841255901331 2216 3 0.21216278064360594 2216 4 0.2875080400232264 2216 5 0.2953735437959111 2216 6 0.23792129876083834 2216 7 0.2230242501612878 2216 8 0.313636551578341 2216 9 0.11449397224228619 2216 10 0.18321657133240876 2216 11 0.14512084330353048 2216 12 -0.013658148266010528 2216 13 -0.03234159255455182 2216 14 0.19493715549577217 2216 15 -0.07513274495560296 2216 16 -0.10683302064958156 2216 17 -0.08285223323406399 2216 18 0.028312919735071773 2216 19 -0.15703871306149608 2216 20 0.027827849887522405 2216 21 0.06608261722656295 2216 22 0.015283034146634122 2216 23 0.14920849630970423 2216 24 0.01697422376380331 2216 25 0.04639240237074205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 60.0 10 161.0 11 219.0 12 297.0 13 378.0 14 623.0 15 947.0 16 1267.0 17 1553.0 18 1657.0 19 1654.0 20 1694.0 21 1695.0 22 1524.0 23 1393.0 24 1521.0 25 1499.0 26 1507.0 27 1526.0 28 1805.0 29 2090.0 30 2701.0 31 3665.0 32 4917.0 33 6917.0 34 9963.0 35 15633.0 36 25935.0 37 52479.0 38 163614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.78805507202543 18.051243110366062 12.28180814883218 22.878893668776325 2 12.002457951021144 24.570180292637726 34.391045851725075 29.036315904616057 3 22.622792627617486 31.365434719675772 27.517128244716783 18.494644407989963 4 13.777830671152596 22.2815843138264 28.068691339281692 35.87189367573931 5 9.841138118810289 40.10562915930897 34.94414584800852 15.10908687387222 6 31.72394270144726 29.616261825949053 18.38623445054574 20.273561022057947 7 19.66812373150123 36.033888819198516 23.724103326160588 20.573884123139667 8 29.006204587341873 31.353912363822335 21.899716628229747 17.740166420606048 9 27.99558699127369 15.82121524996864 20.393761317984296 35.78943644077337 10 19.04798664518059 31.52438573298724 29.573913071447638 19.85371455038453 11 25.121020009713767 27.41436929678127 17.548142644395767 29.916468049109195 12 25.767210573208665 33.09177578570532 27.307260557287094 13.83375308379892 13 32.11074979334125 20.29276388795075 23.007150232068934 24.58933608663907 14 22.452950958349817 28.62183538705496 26.789729140331747 22.135484514263474 15 36.69262364948102 18.91611101997755 24.538837371622297 19.852427958919133 16 15.481555103104224 26.560072563758645 37.56203783222204 20.39633450091509 17 25.096253124004903 28.694527804849805 29.071177456344344 17.138041614800947 18 25.516646885322885 19.749178993821147 34.6196031508625 20.11457096999347 19 21.34519570664428 29.638885940450116 36.264188691504316 12.75172966140129 20 22.986317055754622 22.103069173812635 33.929134314148044 20.9814794562847 21 17.133216896805713 27.20433324005545 37.31662051019784 18.34582935294099 22 20.948027970588424 25.57044432579174 26.65922585542525 26.822301848194584 23 15.298215819285362 25.52082830758542 34.91551918790346 24.26543668522575 24 20.308524633401845 33.502841758899194 29.261914641089227 16.926718966609737 25 20.485752607760077 21.32814836972779 27.84248260689163 30.343616415620506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2109.0 1 2109.0 2 1691.0 3 1273.0 4 1273.0 5 1273.0 6 2934.5 7 4596.0 8 4596.0 9 4596.0 10 4296.0 11 3996.0 12 3996.0 13 3996.0 14 2855.5 15 1715.0 16 1715.0 17 1715.0 18 2724.0 19 3733.0 20 3733.0 21 3733.0 22 5759.5 23 7786.0 24 7786.0 25 7786.0 26 10256.0 27 12726.0 28 12726.0 29 12726.0 30 13857.5 31 14989.0 32 14989.0 33 14989.0 34 14664.5 35 14340.0 36 14340.0 37 14340.0 38 12460.0 39 10580.0 40 10580.0 41 10580.0 42 14177.5 43 17775.0 44 17775.0 45 17775.0 46 58620.5 47 99466.0 48 99466.0 49 99466.0 50 84636.0 51 69806.0 52 69806.0 53 69806.0 54 47361.5 55 24917.0 56 24917.0 57 24917.0 58 16762.5 59 8608.0 60 8608.0 61 8608.0 62 7477.5 63 6347.0 64 6347.0 65 6347.0 66 4903.0 67 3459.0 68 3459.0 69 3459.0 70 2545.0 71 1631.0 72 1631.0 73 1631.0 74 1161.0 75 691.0 76 691.0 77 691.0 78 488.5 79 286.0 80 286.0 81 286.0 82 171.5 83 57.0 84 57.0 85 57.0 86 34.5 87 12.0 88 12.0 89 12.0 90 6.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034094673832981134 2 0.022836998510770378 3 0.002894830797139907 4 3.216478663488786E-4 5 0.0 6 0.011579323188559629 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.216478663488786E-4 21 0.0 22 3.216478663488786E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 310899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.11136092428731 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92016757826138 17.008095876796002 2 11.854125450948446 5.242216925754023 3 4.483300360758758 2.9739561722617314 4 2.150005818689631 1.9015821858545703 5 1.2451995810543466 1.3766528679732004 6 0.8160712207610845 1.0826667181303253 7 0.46840451530315375 0.7249942907503723 8 0.32002792971022925 0.5661002447740263 9 0.2225648783893867 0.4429091119624058 >10 1.2262888397532876 4.9459792408467065 >50 0.10764575817525893 1.7037687480500097 >100 0.1091004305830327 5.276311599587004 >500 0.016001396485511462 2.3930601256356567 >1k 0.04654951704876062 24.59512574823335 >5k 0.011637379262190155 17.36705489564135 >10k+ 0.0029093448155475387 12.399525247749269 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 19785 6.3638030357125634 No Hit GTACATGGGAAGCAGTGGTATCAAC 18765 6.035722212036706 No Hit GAGTACATGGGAAGCAGTGGTATCA 8888 2.858806236108833 No Hit GTATCAACGCAGAGTACTTTTTTTT 8170 2.627863068070338 No Hit CATGTACTCTGCGTTGATACCACTG 7633 2.4551381638409904 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6184 1.9890704055014652 No Hit GGTATCAACGCAGAGTACTTTTTTT 6124 1.9697715335205324 No Hit GCGTTGATACCACTGCTTCCCATGT 5998 1.9292439023605736 No Hit TATCAACGCAGAGTACTTTTTTTTT 5781 1.859446315362867 No Hit TATCAACGCAGAGTACATGGGAAGC 5216 1.6777152708757508 No Hit ACTCTGCGTTGATACCACTGCTTCC 4817 1.5493777722025481 No Hit GTATCAACGCAGAGTACATGGGAAG 4796 1.5426231670092216 No Hit GGTATCAACGCAGAGTACATGGGAA 4762 1.5316871395533598 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3428 1.1026088858439558 No Hit GCTTCCCATGTACTCTGCGTTGATA 3403 1.0945676891852338 No Hit GTGGTATCAACGCAGAGTACATGGG 3335 1.0726956342735101 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3299 1.0611163110849504 No Hit GTACTCTGCGTTGATACCACTGCTT 2794 0.8986841385787668 No Hit ATACCACTGCTTCCCATGTACTCTG 2711 0.8719873656718098 No Hit CAGTGGTATCAACGCAGAGTACATG 2519 0.8102309753328251 No Hit GATACCACTGCTTCCCATGTACTCT 2514 0.8086227360010807 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2513 0.8083010881347318 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2439 0.7844991460249149 No Hit CATGGGAAGCAGTGGTATCAACGCA 2413 0.776136301499844 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2345 0.7542642465881203 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2288 0.7359303182062342 No Hit GCAGTGGTATCAACGCAGAGTACAT 2188 0.7037655315713464 No Hit TACCACTGCTTCCCATGTACTCTGC 2096 0.6741739278672495 No Hit ACATGGGAAGCAGTGGTATCAACGC 2087 0.6712790970701096 No Hit GTTGATACCACTGCTTCCCATGTAC 1981 0.6371844232371284 No Hit CCACTGCTTCCCATGTACTCTGCGT 1951 0.6275349872466621 No Hit CTTCCCATGTACTCTGCGTTGATAC 1898 0.6104876503301715 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1781 0.5728548499673527 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1707 0.5490529078575357 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1561 0.5020923193705995 No Hit CCATGTACTCTGCGTTGATACCACT 1536 0.4940511227118775 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1436 0.46188633607698965 No Hit TTCCCATGTACTCTGCGTTGATACC 1323 0.4255401271795664 No Hit TGATACCACTGCTTCCCATGTACTC 1268 0.407849494530378 No Hit ATCAACGCAGAGTACATGGGAAGCA 1173 0.37729294722723455 No Hit GTGGTATCAACGCAGAGTACTTTTT 1088 0.3499528785875799 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1016 0.3267942322104606 No Hit GTACATGGTAAGCAGTGGTATCAAC 976 0.31392831755650547 No Hit GGAAGCAGTGGTATCAACGCAGAGT 877 0.2820851787879665 No Hit ACCATGTACTCTGCGTTGATACCAC 792 0.25474511014831186 No Hit CTGCGTTGATACCACTGCTTCCCAT 719 0.23126481590484368 No Hit TCAACGCAGAGTACATGGGAAGCAG 643 0.20681957806232892 No Hit AAAAAGTACTCTGCGTTGATACCAC 612 0.1968484942055137 No Hit CCCCATGTACTCTGCGTTGATACCA 600 0.19298871980932716 No Hit TGGGAAGCAGTGGTATCAACGCAGA 579 0.1862341146160007 No Hit CTCTGCGTTGATACCACTGCTTCCC 576 0.18526917101695406 No Hit TCCATGTACTCTGCGTTGATACCAC 547 0.1759413828928366 No Hit GTACATGGAAAGCAGTGGTATCAAC 519 0.16693524263506798 No Hit AACGCAGAGTACATGGGAAGCAGTG 491 0.15792910237729937 No Hit GTACATGGGTAAGCAGTGGTATCAA 491 0.15792910237729937 No Hit AGTGGTATCAACGCAGAGTACATGG 468 0.15053120145127516 No Hit AACGCAGAGTACTTTTTTTTTTTTT 468 0.15053120145127516 No Hit AAAGTACTCTGCGTTGATACCACTG 451 0.14506318772334423 No Hit GGTAAGCAGTGGTATCAACGCAGAG 448 0.1440982441242976 No Hit ACTGCTTCCCATGTACTCTGCGTTG 426 0.13702199106462226 No Hit TCCCATGTACTCTGCGTTGATACCA 411 0.13219727306938908 No Hit CAACGCAGAGTACTTTTTTTTTTTT 406 0.1305890337376447 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 385 0.12383442854431825 No Hit GAGTACATGGTAAGCAGTGGTATCA 370 0.11900971054908506 No Hit ATGTACTCTGCGTTGATACCACTGC 366 0.11772311908368954 No Hit TTGATACCACTGCTTCCCATGTACT 365 0.11740147121734068 No Hit TCAACGCAGAGTACTTTTTTTTTTT 359 0.11547158401924741 No Hit GCTTACCATGTACTCTGCGTTGATA 330 0.10614379589512994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 3.216478663488786E-4 0.0 0.0 0.0 0.0 10 3.216478663488786E-4 0.0 0.0 0.0012865914653955144 0.0 11 3.216478663488786E-4 0.0 0.0 0.0012865914653955144 0.0 12 3.216478663488786E-4 0.0 0.0 0.0012865914653955144 0.0 13 3.216478663488786E-4 0.0 0.0 0.0012865914653955144 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 25 0.006000877 19.011267 1 GCAAAGA 35 0.002165686 16.284887 3 ACGCAAA 65 1.8861101E-7 16.086456 1 ACTTAGA 45 6.7105814E-4 14.78654 1 TGAACAG 40 0.005268316 14.249277 5 TAGATGT 40 0.005268316 14.249277 4 CGCAAAA 50 0.0014917955 13.303603 2 GAACAAA 125 1.0004442E-10 12.927661 1 GAATCAA 65 7.995192E-4 11.691713 18 GAGAACA 70 0.0014782626 10.863581 1 AAAAGTA 105 3.4254517E-6 10.8600855 2 AAAAAGT 110 5.9516206E-6 10.3697815 1 CATGGGT 200 2.582965E-10 9.499517 4 GCAAAAA 90 0.0011087615 9.4995165 3 AATCAAC 105 4.4680826E-4 9.04716 19 ATGGGTA 170 1.6613194E-7 8.940722 5 GGGTAAG 120 1.752203E-4 8.7078905 7 TGGGTAA 135 6.233413E-4 7.7403474 6 AAAGTAC 135 6.233413E-4 7.7403474 3 GAAGCAG 5140 0.0 7.466547 9 >>END_MODULE