FastQCFastQC Report
Wed 25 May 2016
SRR1294887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000577
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52680.13168100501502658No Hit
CCCATGTACTCTGCGTTGATACCAC46650.11660817927014028No Hit
GTATCAACGCAGAGTACTTTTTTTT42460.10613469007095726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA500.001502732913.2971029
CCTGCGA658.042115E-411.6899058
GTTCCGG1001.8979717E-611.4167981
ACCGTTC600.005887395211.0810568
GTCCTAT3400.09.5139981
GTCTTAG4850.09.0235851
AACACCG950.00182464359.0000665
ACTGCGT950.00182817828.9981518
TAGACAG5050.08.8416485
GTCTTAC4650.08.5932881
GTATTAA9000.08.5625981
GTATCAA43200.08.3467721
GTGTAAG6750.08.3159391
TATACTG10200.08.2892765
ACACCGT1150.00111332798.2592766
CTAGACT3700.08.2170984
GTCTCGG1050.00445558748.1548561
CCGCCCG1050.00451730948.14240717
ACTGTCC4200.08.1411858
ATAATAC10800.08.093363
Produced by FastQC (version 0.11.5)