FastQCFastQC Report
Wed 25 May 2016
SRR1294887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000577
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT120110.30023169157848983No Hit
TATCAACGCAGAGTACTTTTTTTTT70290.17569965532471943No Hit
GGTATCAACGCAGAGTACTTTTTTT68270.17065038368215385No Hit
GTACATGGGAAGCAGTGGTATCAAC52750.13185597977491748No Hit
CCCATGTACTCTGCGTTGATACCAC49420.12353218048296534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACCG802.8678855E-511.8754535
AGCACCG1054.1068794E-59.9527615
ACACCGT1252.5715217E-69.8803776
ATACGGT850.00743678.9415176
GTATTAT10000.08.9354791
GTCCTAG4350.08.7409921
GTATTAG6900.08.67923451
GTATTAA9450.08.6508061
TACACCG1107.145812E-48.6366945
GACTCCG1107.153813E-48.6357227
GTCTAGC2106.319169E-98.6005111
TGCGTTG15050.08.26848912
CTAATAC7000.08.143273
ACCGTCT1050.0045180868.1422528
GTATCAA53950.08.1226821
GCCTTAC2605.2023097E-108.0433931
TGGGCCG1304.1865106E-48.0387685
GGCCGTG1304.1915756E-48.0378647
CTAGAAC5700.08.0004053
CTGCGTT15700.07.986667611
Produced by FastQC (version 0.11.5)