FastQCFastQC Report
Wed 25 May 2016
SRR1294886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3685901
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72690.19721093974037826No Hit
CCCATGTACTCTGCGTTGATACCAC68340.18540921202170108No Hit
GTATCAACGCAGAGTACTTTTTTTT46980.12745865936171372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA350.002173737616.28259510
GAACCGT658.040912E-411.6900686
ACAGTCG700.001494628310.8550638
CACCGCG909.5276584E-510.55496817
CCGCGCC900.00111456729.49972819
CGCCCCT800.0045215269.499083514
ACAGCGC1106.8558715E-59.4981818
GTCTTAC4400.09.29923251
GTATTAA7300.09.1244451
TTACACC4100.09.0375554
GTCTAGC2950.09.0316541
ACCGCGC850.00744053958.94092118
TGGACCG850.0074421978.9406785
GTCTAGG3750.08.8811261
GTCTAAT4200.08.8358141
GGAACGA1107.155048E-48.6355315
GTATAGG5200.08.6005411
TAGACAG6100.08.5650965
CTAGCAC3550.08.5644153
GTATTAG6800.08.5359561
Produced by FastQC (version 0.11.5)