FastQCFastQC Report
Wed 25 May 2016
SRR1294886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3685901
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT135030.3663419066328694No Hit
GGTATCAACGCAGAGTACTTTTTTT80220.2176401373775367No Hit
GTACATGGGAAGCAGTGGTATCAAC73660.19984258936960056No Hit
TATCAACGCAGAGTACTTTTTTTTT73080.1982690256737769No Hit
CCCATGTACTCTGCGTTGATACCAC71930.19514902869067835No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39870.10816893888359996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTG600.005875847311.0839425
ACCGTGT2450.010.0808198
ATCCGTC1054.1120064E-59.9515787
GTCTTAG3550.09.6391721
GTCTAGA3850.09.6287391
ACCGTCT850.0074437228.94045458
GTAGGAC2451.6370905E-118.9193413
GCTAGAC2054.138201E-98.80584053
TTAGTAC2701.8189894E-128.7972573
GTGCTAG3600.08.7131861
ACAGCGC1201.766463E-48.707638
TAGGACG1107.1442826E-48.6368394
ACTGTCG1107.1539305E-48.6356678
GTATTAG5950.08.6266541
ACTATAC5400.08.6213123
CTAGACA5650.08.5756924
CTTAGAC3000.08.5509353
CTAGTAC2002.5365807E-88.5509343
TGGGCCG1000.00290491368.550475
ACGGAGT1000.00290848318.5493118
Produced by FastQC (version 0.11.5)