FastQCFastQC Report
Wed 25 May 2016
SRR1294885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265162
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC143325.404997699519539No Hit
CCCATGTACTCTGCGTTGATACCAC138045.205874144862386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA116264.38448948190163No Hit
GAGTACATGGGAAGCAGTGGTATCA68752.5927546179316794No Hit
CATGTACTCTGCGTTGATACCACTG55212.0821233811782984No Hit
GCGTTGATACCACTGCTTCCCATGT44431.6755794570866112No Hit
ACGCAGAGTACATGGGAAGCAGTGG43171.6280613360888814No Hit
TATCAACGCAGAGTACATGGGAAGC41831.5775261915357404No Hit
GGTATCAACGCAGAGTACATGGGAA40021.5092660335945574No Hit
GTATCAACGCAGAGTACATGGGAAG39741.4987064511506174No Hit
ACTCTGCGTTGATACCACTGCTTCC36581.3795340207118667No Hit
GCTTCCCATGTACTCTGCGTTGATA25960.9790241437310021No Hit
GTACTCTGCGTTGATACCACTGCTT24450.9220778241226117No Hit
GTGGTATCAACGCAGAGTACATGGG24370.9190608005672004No Hit
ATACCACTGCTTCCCATGTACTCTG20310.7659468551300714No Hit
GATACCACTGCTTCCCATGTACTCT19620.739925026964648No Hit
GGGAAGCAGTGGTATCAACGCAGAG19270.7267255489097231No Hit
CAGTGGTATCAACGCAGAGTACATG18570.7003265927998733No Hit
CTGCTTCCCATGTACTCTGCGTTGA17540.6614824145239514No Hit
CATGGGAAGCAGTGGTATCAACGCA17040.6426260173026301No Hit
GCAGTGGTATCAACGCAGAGTACAT16400.6184898288593388No Hit
TACCACTGCTTCCCATGTACTCTGC16340.6162270611927803No Hit
GTTGATACCACTGCTTCCCATGTAC15850.5977477919158855No Hit
GCAGAGTACATGGGAAGCAGTGGTA15490.584171185916534No Hit
GTATCAACGCAGAGTACTTTTTTTT14920.5626748930842277No Hit
CCACTGCTTCCCATGTACTCTGCGT14640.5521153106402877No Hit
CTTCCCATGTACTCTGCGTTGATAC14300.5392929605297894No Hit
ACATGGGAAGCAGTGGTATCAACGC13940.525716354530438No Hit
CCATGTACTCTGCGTTGATACCACT12760.48121525708811974No Hit
AAGCAGTGGTATCAACGCAGAGTAC12130.45745619658925485No Hit
GGTATCAACGCAGAGTACTTTTTTT11730.44237107881219784No Hit
ATGGGAAGCAGTGGTATCAACGCAG11460.4321886243126843No Hit
GTACATGGTAAGCAGTGGTATCAAC10760.4057896682028345No Hit
TTCCCATGTACTCTGCGTTGATACC10300.3884417827592189No Hit
TGATACCACTGCTTCCCATGTACTC9590.36166569870494264No Hit
TATCAACGCAGAGTACTTTTTTTTT9120.34394068531690064No Hit
ATCAACGCAGAGTACATGGGAAGCA8710.32847843959541717No Hit
ACCATGTACTCTGCGTTGATACCAC7920.2986853319857295No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7760.29265128487490666No Hit
AAACAAAAAAAAAAAAAAAAAAAAA6820.2572012580988226No Hit
GGAAGCAGTGGTATCAACGCAGAGT6430.242493268266192No Hit
CCCCATGTACTCTGCGTTGATACCA5430.20478047382354939No Hit
CTGCGTTGATACCACTGCTTCCCAT5230.19723791493502085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5130.1934666354907566No Hit
AAAAAGTACTCTGCGTTGATACCAC4930.18592407660222807No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4830.18215279715796381No Hit
CTCTGCGTTGATACCACTGCTTCCC4710.1776272618248467No Hit
TCAACGCAGAGTACATGGGAAGCAG4650.17536449415828814No Hit
GGTAAGCAGTGGTATCAACGCAGAG4510.17008470293631817No Hit
TGGGAAGCAGTGGTATCAACGCAGA4370.16480491171434822No Hit
TCCATGTACTCTGCGTTGATACCAC4130.155753841048114No Hit
GAGTACATGGTAAGCAGTGGTATCA3990.150474049826144No Hit
AAAGTACTCTGCGTTGATACCACTG3890.14670277038187976No Hit
GCTTACCATGTACTCTGCGTTGATA3880.14632564243745333No Hit
TCCCATGTACTCTGCGTTGATACCA3790.1429314909376155No Hit
GTACATGGAAAGCAGTGGTATCAAC3760.14180010710433621No Hit
AGTGGTATCAACGCAGAGTACATGG3430.12935488493826416No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3410.1286006290494113No Hit
GTACATGGGTAAGCAGTGGTATCAA3340.12596073343842631No Hit
ATGTACTCTGCGTTGATACCACTGC3330.12558360549399988No Hit
ACTGCTTCCCATGTACTCTGCGTTG3310.12482934960514705No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3260.12294370988301491No Hit
AACGCAGAGTACATGGGAAGCAGTG3170.11954955838317707No Hit
GAACAAAAAAAAAAAAAAAAAAAAA3020.11389263921678068No Hit
TTGATACCACTGCTTCCCATGTACT3020.11389263921678068No Hit
ACTCTGCGTTGATACCACTGCTTAC2760.1040873126616936No Hit
GAGTACATGGAAAGCAGTGGTATCA2750.10371018471726717No Hit
CTGCTTACCATGTACTCTGCGTTGA2710.10220167293956148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1650.012.1120811
AGTACTC907.406805E-611.6094975
GAGAAAA1053.3591114E-610.8761551
CGCAAAA700.001479057810.8617822
AAGTACT951.6251444E-410.0042724
AAAAGTA951.6251444E-410.0042722
ACGCAAA950.00178443139.0157591
GCAAAAA1107.063192E-48.6400543
AAAGTAC1107.063192E-48.6400543
TACAAAA1107.063192E-48.6400542
ACCATGT3250.08.1989471
GAAGCAG37600.07.52678879
GGAAGCA38150.07.4196768
AGTGGTA41700.07.40165114
CAGTGGT41950.07.334902313
TCCATGT1450.0013075837.21951631
AGCAGTG42850.07.20300711
TGGTATC42850.07.182198516
GTGGTAT43000.07.15579415
AAGCAGT42900.07.15033710
Produced by FastQC (version 0.11.5)