##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294885_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265162
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.28433184242086	31.0	30.0	34.0	16.0	34.0
2	29.4234656549581	31.0	30.0	34.0	16.0	34.0
3	29.78046628099049	31.0	30.0	34.0	22.0	34.0
4	32.76442325823459	37.0	33.0	37.0	19.0	37.0
5	33.64798500539293	37.0	35.0	37.0	27.0	37.0
6	33.47411016661513	37.0	35.0	37.0	25.0	37.0
7	33.657171842119155	37.0	35.0	37.0	27.0	37.0
8	33.63023359304878	37.0	35.0	37.0	26.0	37.0
9	34.89886182786372	39.0	35.0	39.0	23.0	39.0
10	35.11475626220952	39.0	35.0	39.0	27.0	39.0
11	35.094817507787695	39.0	35.0	39.0	26.0	39.0
12	35.26082168636532	39.0	35.0	39.0	27.0	39.0
13	34.87294936680218	39.0	35.0	39.0	25.0	39.0
14	36.02864286737919	40.0	36.0	41.0	25.0	41.0
15	35.963030147607874	40.0	36.0	41.0	25.0	41.0
16	35.81808102216758	40.0	36.0	41.0	25.0	41.0
17	35.89097608254576	40.0	36.0	41.0	25.0	41.0
18	35.760403074347	40.0	36.0	41.0	24.0	41.0
19	35.66755794570866	40.0	35.0	41.0	24.0	41.0
20	35.73215996258891	40.0	35.0	41.0	24.0	41.0
21	35.61459032591397	40.0	35.0	41.0	23.0	41.0
22	35.71243994237485	40.0	35.0	41.0	24.0	41.0
23	35.6845928149584	40.0	35.0	41.0	23.0	41.0
24	35.80219639314834	40.0	35.0	41.0	24.0	41.0
25	34.733536479586064	39.0	33.0	41.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2195503987552101
1101	2	0.12112525743036073
1101	3	0.30065623104010797
1101	4	0.13739577270755632
1101	5	0.03425979180639871
1101	6	0.27351540861067036
1101	7	0.20142523796551615
1101	8	0.12906896469857543
1101	9	0.5289613629788121
1101	10	0.5815345830914538
1101	11	0.15101246596383078
1101	12	0.5162082755592294
1101	13	0.289567837370555
1101	14	0.29527108844388295
1101	15	0.5614518713792975
1101	16	0.06462104719629735
1101	17	0.17575978721323793
1101	18	0.48051149836693696
1101	19	0.004257064862066784
1101	20	0.21549743406207966
1101	21	-0.0019758434226702093
1101	22	0.13365389498572
1101	23	-0.10206546541901673
1101	24	-0.18452176927785047
1101	25	0.05952773370763964
1102	1	-0.3682127591395279
1102	2	-0.3486115846749023
1102	3	-0.94539640053884
1102	4	-0.787341069397705
1102	5	-0.4970559976672888
1102	6	-0.3503003808630112
1102	7	-0.6879168672976377
1102	8	-0.3171152458277362
1102	9	-0.535380742284346
1102	10	-0.6714917326980228
1102	11	-0.5103033235098522
1102	12	-0.38418646128288003
1102	13	-0.20687953105049672
1102	14	-0.38078154313506474
1102	15	-0.6499954970417505
1102	16	-0.444721058066861
1102	17	-0.47516126541833614
1102	18	-0.5226463963699075
1102	19	-0.8021903035589872
1102	20	-1.1150288817273974
1102	21	-0.877370580264774
1102	22	-0.4445039997511202
1102	23	-1.1448286233137495
1102	24	-0.9853112429620552
1102	25	-0.8106038452397257
1103	1	-0.41959185233852025
1103	2	-0.6960297206950514
1103	3	-1.236769196508611
1103	4	-0.8131345202793199
1103	5	-0.8640962999342889
1103	6	-1.1987701541627587
1103	7	-0.9733261871968821
1103	8	-0.7910887974650223
1103	9	-0.3767724299417736
1103	10	-0.8447348057458797
1103	11	-0.6902907290514122
1103	12	-0.7458426325675092
1103	13	-0.7310671884812265
1103	14	-0.6432878403642803
1103	15	-0.8800899554800381
1103	16	-0.37423617141698884
1103	17	-0.7624345651664513
1103	18	-0.26199148453111576
1103	19	-0.4134812355489146
1103	20	-0.7083790076719794
1103	21	-0.43178493794739126
1103	22	-0.5917206244362632
1103	23	-0.8142555754548084
1103	24	-0.5164321497630695
1103	25	-0.31312903415660287
1104	1	-0.5453574291902896
1104	2	-0.5132054933043442
1104	3	-0.5681121365794475
1104	4	-0.47891467345861827
1104	5	-0.5844417844693197
1104	6	-0.15660748746199715
1104	7	-0.4813559535920575
1104	8	-0.32304164176682804
1104	9	0.13704311558367976
1104	10	-0.3860983316827955
1104	11	0.22342764095715495
1104	12	-0.11808240036917539
1104	13	-0.33430338891852074
1104	14	-0.026542964454861817
1104	15	-0.3116579335899772
1104	16	-0.1453175047673696
1104	17	-0.12514857506300814
1104	18	-0.15218954357802517
1104	19	-0.3614923340158427
1104	20	-0.7396989324888139
1104	21	-0.29667261072162887
1104	22	-0.36554460888817886
1104	23	-0.542100196917815
1104	24	-0.23838230895190549
1104	25	-0.6837129822955674
1105	1	0.5446489979664157
1105	2	0.6005486220434442
1105	3	0.6284408087634858
1105	4	0.5613927807314951
1105	5	0.5629827620226351
1105	6	0.734150523529749
1105	7	0.3241438562165726
1105	8	0.5397516792368577
1105	9	0.5658918675347806
1105	10	0.47139586420120594
1105	11	0.32353388579247166
1105	12	0.4129647015078177
1105	13	0.062429254479219765
1105	14	0.4247352527305708
1105	15	0.22035980011587242
1105	16	0.499096771390505
1105	17	0.8300777526695171
1105	18	0.9349246630616221
1105	19	0.6371960013505742
1105	20	0.8494155627170485
1105	21	0.8803102982881938
1105	22	0.9777311423677375
1105	23	0.8225163379265581
1105	24	0.9456835890792874
1105	25	1.1243380152253906
1106	1	-0.7842691895857214
1106	2	-0.9484147342812008
1106	3	-1.2138347207488138
1106	4	-1.1614093108675227
1106	5	-1.5395625593470754
1106	6	-1.0169736092094723
1106	7	-0.9581202793711299
1106	8	-0.8360850621007074
1106	9	-1.6636550204995686
1106	10	-1.422823753695397
1106	11	-1.2693978116988305
1106	12	-1.1654397421227713
1106	13	-0.9916105021791068
1106	14	-1.078143747859471
1106	15	-1.001353764758285
1106	16	-0.9506987483555775
1106	17	-0.9212701893028523
1106	18	-1.0842211995195115
1106	19	-0.9080236368923238
1106	20	-1.248572188814009
1106	21	-1.166038559267399
1106	22	-1.8342284091999446
1106	23	-1.5704519828938004
1106	24	-1.1727521878439475
1106	25	-1.4200067323788375
1107	1	-0.09523656866333852
1107	2	-0.21884967991299575
1107	3	-0.11325354339598093
1107	4	0.006944644888008611
1107	5	-0.09919885481014745
1107	6	0.054342476279842344
1107	7	-0.4401787720595465
1107	8	-0.27794857916106963
1107	9	-0.22216645657005785
1107	10	0.032913029206739
1107	11	-0.3276842758908032
1107	12	-0.2964483660447854
1107	13	-0.17509381676478597
1107	14	-0.4567339240874446
1107	15	-0.005590735136991043
1107	16	-0.19126867711447915
1107	17	-0.18313745589453134
1107	18	-0.12788449160800042
1107	19	-0.03421411308279687
1107	20	-0.04502888172739716
1107	21	-0.295584865979059
1107	22	-0.4961706664177896
1107	23	0.4140999481148171
1107	24	-0.1526921953430076
1107	25	-0.08358003571591865
1108	1	-0.24054367701392465
1108	2	0.00561546846519434
1108	3	-0.26870557928279837
1108	4	0.07526762625446537
1108	5	-0.3239400556383032
1108	6	0.10927691382297411
1108	7	0.24308554816129657
1108	8	0.10828330489690075
1108	9	0.1759356828364318
1108	10	0.4065155136787908
1108	11	0.013344019485316494
1108	12	0.11266136480407596
1108	13	-0.19203653588141378
1108	14	0.16431507522242583
1108	15	0.14216633870704243
1108	16	0.08768232357564898
1108	17	-0.41584967121544025
1108	18	-0.15124543018632863
1108	19	-0.3258980330275847
1108	20	-0.3462366111959909
1108	21	-0.014701498139167768
1108	22	0.022669913292354238
1108	23	-0.23848804360360276
1108	24	-0.09886196759973842
1108	25	-0.39189611577113226
1109	1	0.4261920027652337
1109	2	0.36686460580128966
1109	3	0.1891274089849695
1109	4	0.37361131155467575
1109	5	0.260324954713667
1109	6	0.46386628580365397
1109	7	0.32172598984521983
1109	8	0.33633713512464425
1109	9	0.411166876763275
1109	10	0.35672255301626166
1109	11	0.30088715268062316
1109	12	0.5773611577647415
1109	13	0.8010966594256956
1109	14	0.636123218769697
1109	15	0.5444092648630132
1109	16	0.5730170371712333
1109	17	0.5763863536292817
1109	18	0.5483059845824769
1109	19	0.5110239821553009
1109	20	0.7359234992249881
1109	21	0.4496532292590345
1109	22	0.32763885739173304
1109	23	0.4093380433529177
1109	24	0.5282601856093692
1109	25	0.721181869045985
1110	1	-0.22886344407103465
1110	2	0.21999572126117073
1110	3	-0.15646945989956862
1110	4	-0.1685282840095823
1110	5	0.01068372836010667
1110	6	-0.39366796077168686
1110	7	-0.20185522346201878
1110	8	-0.2521494924030847
1110	9	-0.14783508018388858
1110	10	-0.6337862532459653
1110	11	-0.01617090341852645
1110	12	-0.004471849410734308
1110	13	-0.5060461977171613
1110	14	-0.39815597235880773
1110	15	-0.16201604498695588
1110	16	0.07709401042628627
1110	17	-0.3931178864229068
1110	18	-0.12100256075346749
1110	19	-0.3463798012758801
1110	20	-0.20233481780045537
1110	21	-0.3569025437350959
1110	22	-0.4514218079702985
1110	23	-0.2696573904370396
1110	24	-0.19346192789356564
1110	25	-0.3463001922716913
1111	1	-0.042239565666335466
1111	2	-0.22442743549075317
1111	3	-0.31946399960643745
1111	4	-0.17820354026017782
1111	5	-0.26105366666495655
1111	6	-0.16503814310077303
1111	7	-0.24742152930230077
1111	8	0.10468545347296043
1111	9	-0.13293901734262192
1111	10	-0.38202203572832616
1111	11	-0.2607345589410883
1111	12	-0.4294486990451176
1111	13	-0.43378512545609027
1111	14	-0.4233339906875102
1111	15	-0.7649313944776495
1111	16	-0.5204728063186081
1111	17	-0.36608783884491203
1111	18	-0.4868422005657109
1111	19	-0.5467182755869615
1111	20	-0.3176396043381189
1111	21	-0.07863514902934554
1111	22	-0.13533150557862683
1111	23	-0.14707221305734208
1111	24	-0.042968585619398425
1111	25	-0.3284185305544156
1112	1	0.38505473521713185
1112	2	0.40742679004293336
1112	3	0.4376171434792191
1112	4	0.42132710215985725
1112	5	0.4056866659895988
1112	6	0.5136781744417291
1112	7	0.34234836972267857
1112	8	0.484391526181291
1112	9	-0.20776787546719078
1112	10	0.3431301634645081
1112	11	-0.032075332539200474
1112	12	0.19657538973292077
1112	13	0.23059789558607235
1112	14	-6.122429093267101E-4
1112	15	-0.032281050089153496
1112	16	-0.29366575332645795
1112	17	0.03578117250491175
1112	18	0.13902403252399864
1112	19	-0.0013268724077448724
1112	20	0.3169350008911138
1112	21	-0.15095410170946622
1112	22	0.011579521693569461
1112	23	0.07246821641536627
1112	24	0.12813119496119185
1112	25	-0.20743793101851082
1113	1	0.24110843291345319
1113	2	0.3151301371794055
1113	3	0.233245983567123
1113	4	-0.14442716211294027
1113	5	-0.17262994910216634
1113	6	-0.049599497860803865
1113	7	-0.02691797105039484
1113	8	-0.15752915311250604
1113	9	-0.21108162528655328
1113	10	0.057873609465332265
1113	11	0.13805208487866594
1113	12	0.02464907955597795
1113	13	-0.23893802994674473
1113	14	-0.18107955638009798
1113	15	0.09497690913926249
1113	16	-0.16294953488805675
1113	17	0.11020849175605463
1113	18	0.31514609811133454
1113	19	0.19131411145205135
1113	20	0.28355831474059556
1113	21	0.28262390097143
1113	22	0.33517591194866014
1113	23	0.5111040477679296
1113	24	0.45421414335140753
1113	25	0.5092140355549759
1114	1	0.08867202833250687
1114	2	0.42952040637655386
1114	3	0.34000628402491984
1114	4	-0.06304576738428125
1114	5	0.30503572492779085
1114	6	0.37572310906987383
1114	7	0.1349410745368118
1114	8	0.2577337474608541
1114	9	-0.3455764917250619
1114	10	0.07089641047870998
1114	11	0.27894723577426106
1114	12	-0.13799518611509143
1114	13	-0.40313792031223983
1114	14	-0.047369910025444995
1114	15	0.13474163942357364
1114	16	0.09131361754835154
1114	17	-0.11928318935569848
1114	18	-0.6714607140432847
1114	19	0.0069651774253500776
1114	20	0.04926642028603112
1114	21	0.2865500013907081
1114	22	0.12217385259786795
1114	23	-0.004755916378627489
1114	24	0.35114289574040214
1114	25	0.294256333731191
1115	1	0.6083852364061286
1115	2	0.4816222683317797
1115	3	0.42659691794668575
1115	4	0.9853092646780155
1115	5	0.7732001270543165
1115	6	0.2653510667984591
1115	7	0.6016655380475697
1115	8	0.6080573599629062
1115	9	0.36260923544394075
1115	10	0.31076327843783247
1115	11	0.1595519103431542
1115	12	0.25152066566590037
1115	13	0.2296627852078572
1115	14	-0.013643458502549777
1115	15	0.297315193381408
1115	16	0.5685361802404856
1115	17	0.4723721421540432
1115	18	0.3596698619819918
1115	19	0.47876181225392855
1115	20	0.5497372652659251
1115	21	0.6237597092675173
1115	22	0.6889815236341761
1115	23	0.3714018889356936
1115	24	0.3642767303118859
1115	25	0.40589392758877807
1116	1	0.5142872408604724
1116	2	-0.017755728819043526
1116	3	0.180504500362062
1116	4	0.32193820988157285
1116	5	0.2210070653957743
1116	6	0.04990232183968857
1116	7	-0.1349664168471847
1116	8	-0.029299930512422634
1116	9	-0.26103389543749955
1116	10	-0.1510638047700965
1116	11	0.17514712694060108
1116	12	-0.17366844326485875
1116	13	-0.36415430582527364
1116	14	-0.4622680296215478
1116	15	-0.13551351505977038
1116	16	-0.1145634004092031
1116	17	-0.07869730145437615
1116	18	-0.2682319819554877
1116	19	0.03567005680137214
1116	20	0.053619766921251255
1116	21	-0.09320391359810998
1116	22	0.3592347389876167
1116	23	0.45843714245201284
1116	24	0.2208599282091157
1116	25	0.09788714575126534
1201	1	-0.33415835499445024
1201	2	-0.2492592530686828
1201	3	-0.09863474250775184
1201	4	-0.2772892559262061
1201	5	-0.43143423600925956
1201	6	-0.330261520759386
1201	7	-0.26158267040644745
1201	8	-0.1852888209572754
1201	9	-0.4537356645641353
1201	10	-0.436504686065895
1201	11	-0.3465468468259161
1201	12	-0.04660874107562307
1201	13	0.30051684200649476
1201	14	0.2496070579012084
1201	15	0.2413127931136856
1201	16	0.04902246524920173
1201	17	0.21252008173191683
1201	18	0.03237951036584974
1201	19	-0.12132242791131631
1201	20	-0.017308674473511587
1201	21	-0.34924881860674617
1201	22	-0.5258770567459408
1201	23	-0.4770177424847333
1201	24	-0.3946117610967761
1201	25	-0.5938810473122658
1202	1	-0.22556047487556796
1202	2	0.05786049654337333
1202	3	0.01056806646623798
1202	4	-0.473838531326642
1202	5	0.01708105817027672
1202	6	0.048976268883180296
1202	7	0.20646130529119233
1202	8	0.565283231329623
1202	9	0.42892128817251063
1202	10	0.438774764763906
1202	11	0.4772347475561389
1202	12	0.7093287671435178
1202	13	0.41442757554848697
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2210	3	0.5598886350906156
2210	4	0.5895710446165481
2210	5	0.25354970304530156
2210	6	0.4333041322011226
2210	7	0.45971377403252944
2210	8	0.6500403361200071
2210	9	0.5031643883569856
2210	10	0.3580628991309567
2210	11	0.271608790504402
2210	12	0.36888360997603087
2210	13	0.20100051645544426
2210	14	0.2593728511642226
2210	15	0.14913158134304894
2210	16	0.7028383243559446
2210	17	0.09332448280019179
2210	18	0.32685479127854933
2210	19	0.2519130218566872
2210	20	0.4097217120968324
2210	21	0.530509467241167
2210	22	0.4037501570184716
2210	23	0.1315074811993142
2210	24	0.4897125100070596
2210	25	0.20012655856074701
2211	1	0.08495232399586428
2211	2	-0.010191157121457906
2211	3	0.08245395575569958
2211	4	0.13113874057854247
2211	5	0.1466613420001721
2211	6	0.1506437996595551
2211	7	-0.04147387442353079
2211	8	0.09659615559743884
2211	9	0.11361569477028866
2211	10	0.23454745970102664
2211	11	-0.1701964351488101
2211	12	0.15748218479788534
2211	13	0.18912403189487037
2211	14	0.606166200332872
2211	15	0.16338336281572907
2211	16	0.29666255238444705
2211	17	0.25484467282394263
2211	18	0.2793488530362751
2211	19	0.21390827126286638
2211	20	-0.4050051287582761
2211	21	0.13383488291812284
2211	22	-0.1591952111525501
2211	23	-0.07039275633037789
2211	24	-0.013850435361106861
2211	25	-0.1276644153109885
2212	1	-0.18941646284323
2212	2	0.038425452362137236
2212	3	0.3096961920537531
2212	4	0.08664041208377427
2212	5	0.23664770603641472
2212	6	0.25692184135920826
2212	7	0.5372683178875448
2212	8	-0.19646709767959436
2212	9	0.09350814660454176
2212	10	0.13165641545012363
2212	11	-0.03687739758392894
2212	12	0.22960983501342014
2212	13	-0.0847499014208708
2212	14	0.25107030871679115
2212	15	0.5468563548101031
2212	16	0.39676042341461937
2212	17	0.11243132717425652
2212	18	0.09281656659305781
2212	19	0.09892080755212618
2212	20	0.31237852568001045
2212	21	0.14059238269818763
2212	22	-0.006818814565939135
2212	23	0.009801006315875327
2212	24	0.22792949777868188
2212	25	-0.022733474869355064
2213	1	0.7296452085279093
2213	2	1.1483283691357222
2213	3	0.8592802738260588
2213	4	0.7060538497708819
2213	5	0.6134867275059221
2213	6	0.6687931526242821
2213	7	0.8418348648039782
2213	8	0.35919537772884524
2213	9	1.0774541306948677
2213	10	1.048029052521379
2213	11	0.8430569536264088
2213	12	0.7621241622737074
2213	13	0.6317521086723659
2213	14	0.6108235376963478
2213	15	0.7606800225887298
2213	16	1.2901346001317506
2213	17	0.9717729147202263
2213	18	0.8785545274176272
2213	19	0.5754251698821236
2213	20	0.8622459450624476
2213	21	0.7765035536844209
2213	22	0.8046761388170083
2213	23	0.8583757646770138
2213	24	0.7431067708947552
2213	25	1.10285458432147
2214	1	0.23202917634434428
2214	2	0.465098092744455
2214	3	0.6137049819496418
2214	4	0.46086169557925416
2214	5	0.6084048318258013
2214	6	0.15941160070380533
2214	7	0.6012190774028241
2214	8	0.5643709292874703
2214	9	0.2711515157801756
2214	10	0.3518838433388467
2214	11	0.43552616957770596
2214	12	0.6769771331871652
2214	13	0.46246378692185175
2214	14	0.5518682264501891
2214	15	0.5449468992716149
2214	16	0.564184471887053
2214	17	0.8271544027844229
2214	18	0.7390125898052133
2214	19	0.61655393607235
2214	20	0.8796254961527907
2214	21	1.2320649036061937
2214	22	0.8635835578525644
2214	23	0.49566676839131674
2214	24	0.7121311533513079
2214	25	0.7439929289538227
2215	1	0.3091148270673685
2215	2	0.2516757716469371
2215	3	0.6635116454381738
2215	4	0.6627738731310302
2215	5	0.33897848509133155
2215	6	0.45467088350480367
2215	7	0.24446819017362742
2215	8	0.5364192369308824
2215	9	0.2547629358765775
2215	10	0.5348013707502517
2215	11	0.18389207878900038
2215	12	0.288773308832063
2215	13	0.436482537915019
2215	14	0.4426092614626356
2215	15	0.41296427307319306
2215	16	0.680652505151528
2215	17	0.41883298745522524
2215	18	0.5978133737450406
2215	19	0.34714877690078083
2215	20	0.3934768653990446
2215	21	0.6142673507697083
2215	22	0.4900362301339385
2215	23	0.6127863192149832
2215	24	0.9065853087620823
2215	25	0.8396547754499295
2216	1	0.2194154459886768
2216	2	0.3486106375473206
2216	3	0.8586634285209911
2216	4	0.5853939733373394
2216	5	0.7519610963498096
2216	6	0.419849191786561
2216	7	0.49150620962544167
2216	8	0.3733334721209758
2216	9	0.2852816508780478
2216	10	0.3547689510626597
2216	11	0.5174317192251934
2216	12	0.19079217119575276
2216	13	0.24181705014608212
2216	14	0.2934492260957029
2216	15	0.34831646877021427
2216	16	0.6978644120186104
2216	17	0.5022105294534569
2216	18	0.5246185608950498
2216	19	0.5620618331931482
2216	20	0.6848856481871337
2216	21	0.43671061631642516
2216	22	0.7230601028129797
2216	23	0.8166030006178744
2216	24	1.0197742271234134
2216	25	0.6667252145893343
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72.0
3	47.0
4	11.0
5	18.0
6	13.0
7	34.0
8	60.0
9	156.0
10	284.0
11	443.0
12	762.0
13	1124.0
14	1879.0
15	2780.0
16	3389.0
17	3710.0
18	3637.0
19	3388.0
20	3130.0
21	2923.0
22	2776.0
23	2507.0
24	2413.0
25	2315.0
26	2268.0
27	2296.0
28	2303.0
29	2514.0
30	2981.0
31	3627.0
32	4757.0
33	6325.0
34	8709.0
35	12802.0
36	21498.0
37	45261.0
38	111950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.348879364060345	23.91986556145088	10.50773210475633	22.223522969732446
2	11.741239078334056	30.748617689796383	30.683323583249987	26.826819648619576
3	21.215437092489736	36.7189386621589	23.838218425501086	18.22740581985028
4	13.021126255515586	28.44476486330403	24.94838278646138	33.58572609471901
5	9.995395739927238	45.75275878205999	30.580967045574624	13.670878432438144
6	29.018537807768368	35.736606131968664	16.925939344534516	18.318916715728452
7	19.259566759755455	41.1732960978187	20.26303871990339	19.304098422522454
8	26.20897888320658	38.57749429148346	19.27195184087864	15.94157498443132
9	25.60941091221992	24.50324377568697	17.29372115228574	32.59362415980737
10	17.221819663964492	38.232341530425856	26.347689567199556	18.198149238410096
11	24.044623246054332	34.39356314204412	15.418871285484615	26.14294232641693
12	23.040111109433735	39.66576591536963	23.372609109162	13.921513866034632
13	27.98745461544849	28.663033386423507	19.09849862997154	24.251013368156464
14	20.204841010468478	36.34682587004596	21.456605254617227	21.99172786486833
15	33.03783710345765	27.87514435160655	19.135550875921776	19.95146766901403
16	15.615930679729633	34.3499148969895	30.562359183765892	19.471795239514968
17	24.593424593424594	36.37835573319445	22.785584075906655	16.242635597474305
18	24.565508652086123	29.196686354801578	26.664276413865984	19.573528579246315
19	21.68787008487657	37.21662207092798	27.36958105165432	13.725926792541127
20	23.50214760743699	31.035380794577005	24.662384032972756	20.800087565013246
21	18.695744070534595	34.30182526382535	28.160620197170765	18.841810468469284
22	21.975927442639904	33.17619617360322	18.500541613669046	26.347334770087826
23	16.639301257299017	33.84176376668818	25.6853619594388	23.833573016574004
24	21.002763223458707	40.21396107327827	20.76041493650626	18.02286076675676
25	21.238246584856732	29.504799507781502	19.195011380665324	30.061942526696438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12181.0
1	12181.0
2	8330.0
3	4479.0
4	4479.0
5	4479.0
6	4282.0
7	4085.0
8	4085.0
9	4085.0
10	3790.5
11	3496.0
12	3496.0
13	3496.0
14	3402.5
15	3309.0
16	3309.0
17	3309.0
18	3706.0
19	4103.0
20	4103.0
21	4103.0
22	4712.0
23	5321.0
24	5321.0
25	5321.0
26	6238.0
27	7155.0
28	7155.0
29	7155.0
30	7851.0
31	8547.0
32	8547.0
33	8547.0
34	9002.0
35	9457.0
36	9457.0
37	9457.0
38	9480.0
39	9503.0
40	9503.0
41	9503.0
42	13188.5
43	16874.0
44	16874.0
45	16874.0
46	48469.5
47	80065.0
48	80065.0
49	80065.0
50	67054.5
51	54044.0
52	54044.0
53	54044.0
54	37465.5
55	20887.0
56	20887.0
57	20887.0
58	14804.5
59	8722.0
60	8722.0
61	8722.0
62	7469.5
63	6217.0
64	6217.0
65	6217.0
66	4824.0
67	3431.0
68	3431.0
69	3431.0
70	2645.5
71	1860.0
72	1860.0
73	1860.0
74	1374.0
75	888.0
76	888.0
77	888.0
78	621.0
79	354.0
80	354.0
81	354.0
82	240.5
83	127.0
84	127.0
85	127.0
86	82.0
87	37.0
88	37.0
89	37.0
90	27.0
91	17.0
92	17.0
93	17.0
94	9.5
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13463467616023414
2	0.0780654844962702
3	0.0520436563308468
4	0.08711655516250442
5	0.07165430944102096
6	0.07165430944102096
7	0.06863728588560955
8	0.0780654844962702
9	0.07278569327430025
10	0.07165430944102096
11	0.07090005355216811
12	0.07467133299643237
13	0.07693410066299093
14	0.06637451821905099
15	0.06939154177446241
16	0.07127718149659454
17	0.07731122860741735
18	0.07429420505200594
19	0.07127718149659454
20	0.08145963599610803
21	0.08070538010725518
22	0.08032825216282877
23	0.08636229927365158
24	0.09579049788431224
25	0.09013357871791584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	265162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.76072740437921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.48893160966513	27.35158129747098
2	9.832045954252957	6.44209954669221
3	3.0908609515477328	3.0377655923548623
4	1.2282862701308868	1.6095820668119865
5	0.6584627427505785	1.0785859210595787
6	0.3280802127341169	0.6448887849691887
7	0.1991504449228148	0.45670194070040204
8	0.14734830607005953	0.38617901509266034
9	0.10590659498785529	0.3122619379850808
>10	0.7114160402445061	4.635279564945203
>50	0.07942994624089146	1.8381216011344008
>100	0.07942994624089146	6.286345705644098
>500	0.0115115864117234	2.7206009910922377
>1k	0.03338360059399786	23.529766708653575
>5k	0.00230231728234468	4.674877999109978
>10k+	0.0034534759235170204	14.995361326283554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	14332	5.404997699519539	No Hit
CCCATGTACTCTGCGTTGATACCAC	13804	5.205874144862386	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	11626	4.38448948190163	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6875	2.5927546179316794	No Hit
CATGTACTCTGCGTTGATACCACTG	5521	2.0821233811782984	No Hit
GCGTTGATACCACTGCTTCCCATGT	4443	1.6755794570866112	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4317	1.6280613360888814	No Hit
TATCAACGCAGAGTACATGGGAAGC	4183	1.5775261915357404	No Hit
GGTATCAACGCAGAGTACATGGGAA	4002	1.5092660335945574	No Hit
GTATCAACGCAGAGTACATGGGAAG	3974	1.4987064511506174	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3658	1.3795340207118667	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2596	0.9790241437310021	No Hit
GTACTCTGCGTTGATACCACTGCTT	2445	0.9220778241226117	No Hit
GTGGTATCAACGCAGAGTACATGGG	2437	0.9190608005672004	No Hit
ATACCACTGCTTCCCATGTACTCTG	2031	0.7659468551300714	No Hit
GATACCACTGCTTCCCATGTACTCT	1962	0.739925026964648	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1927	0.7267255489097231	No Hit
CAGTGGTATCAACGCAGAGTACATG	1857	0.7003265927998733	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1754	0.6614824145239514	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1704	0.6426260173026301	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1640	0.6184898288593388	No Hit
TACCACTGCTTCCCATGTACTCTGC	1634	0.6162270611927803	No Hit
GTTGATACCACTGCTTCCCATGTAC	1585	0.5977477919158855	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1549	0.584171185916534	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1492	0.5626748930842277	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1464	0.5521153106402877	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1430	0.5392929605297894	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1394	0.525716354530438	No Hit
CCATGTACTCTGCGTTGATACCACT	1276	0.48121525708811974	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1213	0.45745619658925485	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1173	0.44237107881219784	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	1146	0.4321886243126843	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1076	0.4057896682028345	No Hit
TTCCCATGTACTCTGCGTTGATACC	1030	0.3884417827592189	No Hit
TGATACCACTGCTTCCCATGTACTC	959	0.36166569870494264	No Hit
TATCAACGCAGAGTACTTTTTTTTT	912	0.34394068531690064	No Hit
ATCAACGCAGAGTACATGGGAAGCA	871	0.32847843959541717	No Hit
ACCATGTACTCTGCGTTGATACCAC	792	0.2986853319857295	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	776	0.29265128487490666	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	682	0.2572012580988226	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	643	0.242493268266192	No Hit
CCCCATGTACTCTGCGTTGATACCA	543	0.20478047382354939	No Hit
CTGCGTTGATACCACTGCTTCCCAT	523	0.19723791493502085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	513	0.1934666354907566	No Hit
AAAAAGTACTCTGCGTTGATACCAC	493	0.18592407660222807	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	483	0.18215279715796381	No Hit
CTCTGCGTTGATACCACTGCTTCCC	471	0.1776272618248467	No Hit
TCAACGCAGAGTACATGGGAAGCAG	465	0.17536449415828814	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	451	0.17008470293631817	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	437	0.16480491171434822	No Hit
TCCATGTACTCTGCGTTGATACCAC	413	0.155753841048114	No Hit
GAGTACATGGTAAGCAGTGGTATCA	399	0.150474049826144	No Hit
AAAGTACTCTGCGTTGATACCACTG	389	0.14670277038187976	No Hit
GCTTACCATGTACTCTGCGTTGATA	388	0.14632564243745333	No Hit
TCCCATGTACTCTGCGTTGATACCA	379	0.1429314909376155	No Hit
GTACATGGAAAGCAGTGGTATCAAC	376	0.14180010710433621	No Hit
AGTGGTATCAACGCAGAGTACATGG	343	0.12935488493826416	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	341	0.1286006290494113	No Hit
GTACATGGGTAAGCAGTGGTATCAA	334	0.12596073343842631	No Hit
ATGTACTCTGCGTTGATACCACTGC	333	0.12558360549399988	No Hit
ACTGCTTCCCATGTACTCTGCGTTG	331	0.12482934960514705	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	326	0.12294370988301491	No Hit
AACGCAGAGTACATGGGAAGCAGTG	317	0.11954955838317707	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	302	0.11389263921678068	No Hit
TTGATACCACTGCTTCCCATGTACT	302	0.11389263921678068	No Hit
ACTCTGCGTTGATACCACTGCTTAC	276	0.1040873126616936	No Hit
GAGTACATGGAAAGCAGTGGTATCA	275	0.10371018471726717	No Hit
CTGCTTACCATGTACTCTGCGTTGA	271	0.10220167293956148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	3.771279444264261E-4	0.0
13	0.0	0.0	0.0	3.771279444264261E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACAAA	165	0.0	12.112081	1
AGTACTC	90	7.406805E-6	11.609497	5
GAGAAAA	105	3.3591114E-6	10.876155	1
CGCAAAA	70	0.0014790578	10.861782	2
AAGTACT	95	1.6251444E-4	10.004272	4
AAAAGTA	95	1.6251444E-4	10.004272	2
ACGCAAA	95	0.0017844313	9.015759	1
GCAAAAA	110	7.063192E-4	8.640054	3
AAAGTAC	110	7.063192E-4	8.640054	3
TACAAAA	110	7.063192E-4	8.640054	2
ACCATGT	325	0.0	8.198947	1
GAAGCAG	3760	0.0	7.5267887	9
GGAAGCA	3815	0.0	7.419676	8
AGTGGTA	4170	0.0	7.401651	14
CAGTGGT	4195	0.0	7.3349023	13
TCCATGT	145	0.001307583	7.2195163	1
AGCAGTG	4285	0.0	7.203007	11
TGGTATC	4285	0.0	7.1821985	16
GTGGTAT	4300	0.0	7.155794	15
AAGCAGT	4290	0.0	7.150337	10
>>END_MODULE