FastQCFastQC Report
Wed 25 May 2016
SRR1294885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265162
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC150255.666347365007052No Hit
GTACATGGGAAGCAGTGGTATCAAC143885.426116864407419No Hit
GAGTACATGGGAAGCAGTGGTATCA69762.6308445403187486No Hit
GTATCAACGCAGAGTACTTTTTTTT58722.2144952896719743No Hit
CATGTACTCTGCGTTGATACCACTG57992.186964949728845No Hit
ACGCAGAGTACATGGGAAGCAGTGG48571.8317104260791517No Hit
GCGTTGATACCACTGCTTCCCATGT48171.8166253083020945No Hit
GGTATCAACGCAGAGTACTTTTTTT45701.7234747060287672No Hit
TATCAACGCAGAGTACATGGGAAGC42541.6043022755900167No Hit
ACTCTGCGTTGATACCACTGCTTCC40231.517185720427512No Hit
TATCAACGCAGAGTACTTTTTTTTT39801.500969218817176No Hit
GGTATCAACGCAGAGTACATGGGAA39311.482489949540281No Hit
GTATCAACGCAGAGTACATGGGAAG39071.473438878874047No Hit
GCTTCCCATGTACTCTGCGTTGATA27431.0344619515616869No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25250.9522480596767259No Hit
GTGGTATCAACGCAGAGTACATGGG24780.934523046288684No Hit
GTACTCTGCGTTGATACCACTGCTT23490.8858735414576749No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22700.8560804338479873No Hit
ATACCACTGCTTCCCATGTACTCTG21950.8277958380160053No Hit
GGGAAGCAGTGGTATCAACGCAGAG21110.7961170906841855No Hit
GATACCACTGCTTCCCATGTACTCT20440.770849518407615No Hit
CAGTGGTATCAACGCAGAGTACATG20330.7667011110189242No Hit
CATGGGAAGCAGTGGTATCAACGCA19140.7218228856321796No Hit
CTGCTTCCCATGTACTCTGCGTTGA18350.6920297780224919No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17830.6724191249123178No Hit
GCAGAGTACATGGGAAGCAGTGGTA17600.6637451821905099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17270.6512999600244379No Hit
GCAGTGGTATCAACGCAGAGTACAT17220.6494143203023057No Hit
TACCACTGCTTCCCATGTACTCTGC16690.6294265392477052No Hit
ACATGGGAAGCAGTGGTATCAACGC15630.589450977138504No Hit
CCATGTACTCTGCGTTGATACCACT15540.5860568256386662No Hit
GTTGATACCACTGCTTCCCATGTAC14620.5513610547514349No Hit
CCACTGCTTCCCATGTACTCTGCGT14350.5411786002519214No Hit
CTTCCCATGTACTCTGCGTTGATAC14220.5362759369743779No Hit
AAGCAGTGGTATCAACGCAGAGTAC13270.5004487822538675No Hit
ATGGGAAGCAGTGGTATCAACGCAG12490.4710328025886062No Hit
GTACATGGTAAGCAGTGGTATCAAC10830.4084295638138195No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10340.3899502945369246No Hit
TTCCCATGTACTCTGCGTTGATACC10150.3827848635928225No Hit
TGATACCACTGCTTCCCATGTACTC10120.3816534797595432No Hit
ATCAACGCAGAGTACATGGGAAGCA9680.3650598502047805No Hit
ACCATGTACTCTGCGTTGATACCAC8940.33715238231722494No Hit
GTGGTATCAACGCAGAGTACTTTTT7390.2786975509311289No Hit
ATCAACGCAGAGTACTTTTTTTTTT7100.2677608405427625No Hit
GGAAGCAGTGGTATCAACGCAGAGT6810.2568241301543962No Hit
TCAACGCAGAGTACATGGGAAGCAG5150.19422089137960946No Hit
CCCCATGTACTCTGCGTTGATACCA5110.19271237960190374No Hit
CTGCGTTGATACCACTGCTTCCCAT5090.19195812371305088No Hit
AAAAAGTACTCTGCGTTGATACCAC4980.1878097163243602No Hit
GGTAAGCAGTGGTATCAACGCAGAG4960.18705546043550736No Hit
TGGGAAGCAGTGGTATCAACGCAGA4840.18252992510239024No Hit
TCCATGTACTCTGCGTTGATACCAC4750.17913577360255242No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4620.17423311032500885No Hit
CTCTGCGTTGATACCACTGCTTCCC4540.17121608676959743No Hit
GAGTACATGGTAAGCAGTGGTATCA4310.16254214404778966No Hit
AAAGTACTCTGCGTTGATACCACTG4280.16141076021451037No Hit
TCCCATGTACTCTGCGTTGATACCA4050.15273681749270257No Hit
GTACATGGAAAGCAGTGGTATCAAC3840.1448171306597476No Hit
GCTTACCATGTACTCTGCGTTGATA3750.14142297915990978No Hit
AACGCAGAGTACATGGGAAGCAGTG3660.13802882766007193No Hit
GTACATGGGTAAGCAGTGGTATCAA3490.13161765260482272No Hit
AGTGGTATCAACGCAGAGTACATGG3410.1286006290494113No Hit
TTGATACCACTGCTTCCCATGTACT3330.12558360549399988No Hit
AACGCAGAGTACTTTTTTTTTTTTT3150.11879530249432421No Hit
ATGTACTCTGCGTTGATACCACTGC3110.11728679071661852No Hit
CAACGCAGAGTACTTTTTTTTTTTT3070.11577827893891281No Hit
CAACGCAGAGTACATGGGAAGCAGT3030.11426976716120711No Hit
ACGCAGAGTACATGGTAAGCAGTGG3020.11389263921678068No Hit
ACTGCTTCCCATGTACTCTGCGTTG3000.11313838332792783No Hit
GCGTTGATACCACTGCTTACCATGT2810.10597295238382574No Hit
TATCAACGCAGAGTACATGGTAAGC2720.10257880088398791No Hit
ACTCTGCGTTGATACCACTGCTTAC2670.10069316116185577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGAA456.737721E-414.7773383
ACGCAAA400.00525171414.2549511
TACATGA500.001492046913.3021122
AAAAAGT951.3431485E-511.0038221
ATGGGTA1408.203642E-910.856825
GAGAACA1158.671559E-710.7428631
AGAACAA803.7429953E-410.6891972
TGGGTAA1252.2098357E-710.6396836
GAACAAA1105.958371E-610.3672381
AAAAGTA1054.0623294E-59.9539612
GGGTAAG1554.195208E-79.1932757
AGTACTC1356.731681E-58.4441935
AAAGTAC1252.720805E-48.3597513
CATGGGT2407.95626E-97.9164314
ATGGGGG1100.00681616137.7724965
AAGTACT1356.2231463E-47.74051054
TAAAAAA1250.00249950567.6026411
CCATGTA39800.07.5200382
CCCATGT38900.07.0602931
TACTCTG44550.07.03682767
Produced by FastQC (version 0.11.5)