##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265162 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36637979801027 31.0 31.0 34.0 28.0 34.0 2 31.543950490643457 33.0 31.0 34.0 28.0 34.0 3 31.672045768247337 33.0 31.0 34.0 28.0 34.0 4 35.064217346376935 37.0 35.0 37.0 32.0 37.0 5 35.42996734072001 37.0 35.0 37.0 33.0 37.0 6 35.09855484571696 37.0 35.0 37.0 32.0 37.0 7 35.062195940594805 37.0 35.0 37.0 32.0 37.0 8 35.08769356091748 37.0 35.0 37.0 32.0 37.0 9 36.62691486713783 39.0 37.0 39.0 32.0 39.0 10 36.709634110468315 39.0 37.0 39.0 32.0 39.0 11 36.71910379315287 39.0 37.0 39.0 32.0 39.0 12 36.77270121661475 39.0 37.0 39.0 32.0 39.0 13 36.57027024988498 39.0 37.0 39.0 32.0 39.0 14 37.814396482150535 40.0 38.0 41.0 32.0 41.0 15 37.73393623520715 40.0 38.0 41.0 32.0 41.0 16 37.58604928307978 40.0 38.0 41.0 32.0 41.0 17 37.794574637391484 40.0 38.0 41.0 32.0 41.0 18 37.80747241309086 40.0 38.0 41.0 32.0 41.0 19 37.738344860877504 40.0 38.0 41.0 32.0 41.0 20 37.83082417540975 40.0 38.0 41.0 32.0 41.0 21 37.75563617712945 40.0 38.0 41.0 32.0 41.0 22 37.86690777713247 40.0 38.0 41.0 32.0 41.0 23 37.785203007972484 40.0 38.0 41.0 32.0 41.0 24 37.80431962347546 40.0 38.0 41.0 32.0 41.0 25 36.67599806910493 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2567636099514665 1101 2 0.17058682079623821 1101 3 -0.0804949915179769 1101 4 -0.1352027488308849 1101 5 -0.032440272422348926 1101 6 -0.16690214235904222 1101 7 -0.1009007014332326 1101 8 0.3583397275802298 1101 9 0.1634573979734597 1101 10 0.20307443129689062 1101 11 0.6139843884699019 1101 12 0.0933764021646013 1101 13 -0.155008948878816 1101 14 -0.01295033175148319 1101 15 0.2299136578306431 1101 16 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-0.2201927968886821 2216 15 -0.3303202602980093 2216 16 -0.2098716169535919 2216 17 -0.21660380250119005 2216 18 -0.1885738724032251 2216 19 0.11840840244292394 2216 20 0.15798343508273405 2216 21 -0.0268680763825202 2216 22 -0.2800022918794767 2216 23 0.09034955512445464 2216 24 -0.0033084782806866997 2216 25 -0.14222295656791317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 12.0 9 57.0 10 92.0 11 130.0 12 185.0 13 273.0 14 455.0 15 748.0 16 1091.0 17 1323.0 18 1398.0 19 1386.0 20 1384.0 21 1357.0 22 1292.0 23 1281.0 24 1366.0 25 1317.0 26 1335.0 27 1445.0 28 1668.0 29 1988.0 30 2543.0 31 3315.0 32 4456.0 33 6174.0 34 8986.0 35 13564.0 36 22451.0 37 45729.0 38 136360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.3360345269893 18.91740987218374 11.988999049300556 22.7575565515264 2 12.521877546093727 24.932481366365913 33.74309725700836 28.802543830532002 3 22.51064455666223 31.197507910348133 27.285515483162932 19.006332049826707 4 13.486849548577851 22.652567109917708 28.41131082130924 35.4492725201952 5 10.287673196008477 40.133955845860264 34.618459658623784 14.95991129950747 6 31.784305294681182 30.37143482163736 17.840789940181192 20.003469943500267 7 20.03680768737602 36.129611331940474 23.242772342945067 20.59080863773844 8 28.73601798146039 31.941228381140586 21.564930118191896 17.757823519207125 9 27.553344747739118 16.085638213620353 20.487098453021172 35.87391858561936 10 19.048355345034356 31.552032342492513 29.432573294816 19.967039017657132 11 25.904541374706785 27.217323749255172 17.933565141309842 28.944569734728205 12 25.375053740732078 33.28644375890965 27.23014609936567 14.1083564009926 13 31.449453541608523 20.79445772772871 23.071933384119898 24.68415534654287 14 22.698576719137733 28.630045029076562 26.20247245080366 22.468905800982043 15 35.721181768126655 19.8995331156048 24.030215490907445 20.3490696253611 16 16.151635603894977 27.077786409817396 35.887495191618704 20.88308279466892 17 25.016782193526975 29.334897157209554 28.21595854609635 17.432362103167122 18 25.069202977802252 21.07277815071541 33.343012950573616 20.515005920908727 19 21.879454823843535 29.902474713571326 34.665977779621514 13.552092682963623 20 23.508647543765697 22.975011502402303 32.57103204833272 20.945308905499278 21 18.04330937313793 27.361386624026068 35.53337205180229 19.06193195103371 22 21.519674764860728 26.170039447582987 25.995429209313553 26.314856578242736 23 15.993618995180306 26.392922062739004 33.53685671400879 24.076602228071895 24 20.499166547242815 33.442951855846616 28.37472941069987 17.683152186210695 25 20.82500509122725 22.669914995361324 26.680670684336366 29.824409229075055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2058.0 1 2058.0 2 1667.0 3 1276.0 4 1276.0 5 1276.0 6 2307.5 7 3339.0 8 3339.0 9 3339.0 10 3165.5 11 2992.0 12 2992.0 13 2992.0 14 2276.5 15 1561.0 16 1561.0 17 1561.0 18 2384.0 19 3207.0 20 3207.0 21 3207.0 22 4784.5 23 6362.0 24 6362.0 25 6362.0 26 8316.0 27 10270.0 28 10270.0 29 10270.0 30 11402.5 31 12535.0 32 12535.0 33 12535.0 34 12575.5 35 12616.0 36 12616.0 37 12616.0 38 11417.5 39 10219.0 40 10219.0 41 10219.0 42 13612.5 43 17006.0 44 17006.0 45 17006.0 46 49569.0 47 82132.0 48 82132.0 49 82132.0 50 69670.5 51 57209.0 52 57209.0 53 57209.0 54 39427.0 55 21645.0 56 21645.0 57 21645.0 58 15256.0 59 8867.0 60 8867.0 61 8867.0 62 7468.5 63 6070.0 64 6070.0 65 6070.0 66 4630.5 67 3191.0 68 3191.0 69 3191.0 70 2409.0 71 1627.0 72 1627.0 73 1627.0 74 1140.5 75 654.0 76 654.0 77 654.0 78 457.5 79 261.0 80 261.0 81 261.0 82 158.0 83 55.0 84 55.0 85 55.0 86 32.0 87 9.0 88 9.0 89 9.0 90 5.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035450026776084054 2 0.018856397221321305 3 0.0011313838332792782 4 0.0 5 0.0 6 0.010559582443939931 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 265162.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.07279323583319 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8381112984823 23.792624885918798 2 10.847061875729667 6.30708774258755 3 3.42846024127643 2.9902474713571325 4 1.4541445064210663 1.6910417028080948 5 0.6395122583992736 0.9296203830111405 6 0.39823582825269166 0.6946696736334769 7 0.19846932157218836 0.40390402848070234 8 0.14528473213127513 0.3379066382060778 9 0.1076663639901414 0.2817145744865403 >10 0.7212349202231159 4.102020651526237 >50 0.0804254767155273 1.6736938173644789 >100 0.07912829160721235 5.792685226389905 >500 0.010377480866519653 2.084386148844857 >1k 0.04540147879102348 30.79362804625097 >5k 0.0038915553249448697 7.032304779719567 >10k+ 0.002594370216629913 11.092464229414471 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 15025 5.666347365007052 No Hit GTACATGGGAAGCAGTGGTATCAAC 14388 5.426116864407419 No Hit GAGTACATGGGAAGCAGTGGTATCA 6976 2.6308445403187486 No Hit GTATCAACGCAGAGTACTTTTTTTT 5872 2.2144952896719743 No Hit CATGTACTCTGCGTTGATACCACTG 5799 2.186964949728845 No Hit ACGCAGAGTACATGGGAAGCAGTGG 4857 1.8317104260791517 No Hit GCGTTGATACCACTGCTTCCCATGT 4817 1.8166253083020945 No Hit GGTATCAACGCAGAGTACTTTTTTT 4570 1.7234747060287672 No Hit TATCAACGCAGAGTACATGGGAAGC 4254 1.6043022755900167 No Hit ACTCTGCGTTGATACCACTGCTTCC 4023 1.517185720427512 No Hit TATCAACGCAGAGTACTTTTTTTTT 3980 1.500969218817176 No Hit GGTATCAACGCAGAGTACATGGGAA 3931 1.482489949540281 No Hit GTATCAACGCAGAGTACATGGGAAG 3907 1.473438878874047 No Hit GCTTCCCATGTACTCTGCGTTGATA 2743 1.0344619515616869 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2525 0.9522480596767259 No Hit GTGGTATCAACGCAGAGTACATGGG 2478 0.934523046288684 No Hit GTACTCTGCGTTGATACCACTGCTT 2349 0.8858735414576749 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2270 0.8560804338479873 No Hit ATACCACTGCTTCCCATGTACTCTG 2195 0.8277958380160053 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2111 0.7961170906841855 No Hit GATACCACTGCTTCCCATGTACTCT 2044 0.770849518407615 No Hit CAGTGGTATCAACGCAGAGTACATG 2033 0.7667011110189242 No Hit CATGGGAAGCAGTGGTATCAACGCA 1914 0.7218228856321796 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1835 0.6920297780224919 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1783 0.6724191249123178 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1760 0.6637451821905099 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1727 0.6512999600244379 No Hit GCAGTGGTATCAACGCAGAGTACAT 1722 0.6494143203023057 No Hit TACCACTGCTTCCCATGTACTCTGC 1669 0.6294265392477052 No Hit ACATGGGAAGCAGTGGTATCAACGC 1563 0.589450977138504 No Hit CCATGTACTCTGCGTTGATACCACT 1554 0.5860568256386662 No Hit GTTGATACCACTGCTTCCCATGTAC 1462 0.5513610547514349 No Hit CCACTGCTTCCCATGTACTCTGCGT 1435 0.5411786002519214 No Hit CTTCCCATGTACTCTGCGTTGATAC 1422 0.5362759369743779 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1327 0.5004487822538675 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1249 0.4710328025886062 No Hit GTACATGGTAAGCAGTGGTATCAAC 1083 0.4084295638138195 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1034 0.3899502945369246 No Hit TTCCCATGTACTCTGCGTTGATACC 1015 0.3827848635928225 No Hit TGATACCACTGCTTCCCATGTACTC 1012 0.3816534797595432 No Hit ATCAACGCAGAGTACATGGGAAGCA 968 0.3650598502047805 No Hit ACCATGTACTCTGCGTTGATACCAC 894 0.33715238231722494 No Hit GTGGTATCAACGCAGAGTACTTTTT 739 0.2786975509311289 No Hit ATCAACGCAGAGTACTTTTTTTTTT 710 0.2677608405427625 No Hit GGAAGCAGTGGTATCAACGCAGAGT 681 0.2568241301543962 No Hit TCAACGCAGAGTACATGGGAAGCAG 515 0.19422089137960946 No Hit CCCCATGTACTCTGCGTTGATACCA 511 0.19271237960190374 No Hit CTGCGTTGATACCACTGCTTCCCAT 509 0.19195812371305088 No Hit AAAAAGTACTCTGCGTTGATACCAC 498 0.1878097163243602 No Hit GGTAAGCAGTGGTATCAACGCAGAG 496 0.18705546043550736 No Hit TGGGAAGCAGTGGTATCAACGCAGA 484 0.18252992510239024 No Hit TCCATGTACTCTGCGTTGATACCAC 475 0.17913577360255242 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 462 0.17423311032500885 No Hit CTCTGCGTTGATACCACTGCTTCCC 454 0.17121608676959743 No Hit GAGTACATGGTAAGCAGTGGTATCA 431 0.16254214404778966 No Hit AAAGTACTCTGCGTTGATACCACTG 428 0.16141076021451037 No Hit TCCCATGTACTCTGCGTTGATACCA 405 0.15273681749270257 No Hit GTACATGGAAAGCAGTGGTATCAAC 384 0.1448171306597476 No Hit GCTTACCATGTACTCTGCGTTGATA 375 0.14142297915990978 No Hit AACGCAGAGTACATGGGAAGCAGTG 366 0.13802882766007193 No Hit GTACATGGGTAAGCAGTGGTATCAA 349 0.13161765260482272 No Hit AGTGGTATCAACGCAGAGTACATGG 341 0.1286006290494113 No Hit TTGATACCACTGCTTCCCATGTACT 333 0.12558360549399988 No Hit AACGCAGAGTACTTTTTTTTTTTTT 315 0.11879530249432421 No Hit ATGTACTCTGCGTTGATACCACTGC 311 0.11728679071661852 No Hit CAACGCAGAGTACTTTTTTTTTTTT 307 0.11577827893891281 No Hit CAACGCAGAGTACATGGGAAGCAGT 303 0.11426976716120711 No Hit ACGCAGAGTACATGGTAAGCAGTGG 302 0.11389263921678068 No Hit ACTGCTTCCCATGTACTCTGCGTTG 300 0.11313838332792783 No Hit GCGTTGATACCACTGCTTACCATGT 281 0.10597295238382574 No Hit TATCAACGCAGAGTACATGGTAAGC 272 0.10257880088398791 No Hit ACTCTGCGTTGATACCACTGCTTAC 267 0.10069316116185577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 3.771279444264261E-4 0.0 0.0 0.0 13 0.0 3.771279444264261E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGAA 45 6.737721E-4 14.777338 3 ACGCAAA 40 0.005251714 14.254951 1 TACATGA 50 0.0014920469 13.302112 2 AAAAAGT 95 1.3431485E-5 11.003822 1 ATGGGTA 140 8.203642E-9 10.85682 5 GAGAACA 115 8.671559E-7 10.742863 1 AGAACAA 80 3.7429953E-4 10.689197 2 TGGGTAA 125 2.2098357E-7 10.639683 6 GAACAAA 110 5.958371E-6 10.367238 1 AAAAGTA 105 4.0623294E-5 9.953961 2 GGGTAAG 155 4.195208E-7 9.193275 7 AGTACTC 135 6.731681E-5 8.444193 5 AAAGTAC 125 2.720805E-4 8.359751 3 CATGGGT 240 7.95626E-9 7.916431 4 ATGGGGG 110 0.0068161613 7.772496 5 AAGTACT 135 6.2231463E-4 7.7405105 4 TAAAAAA 125 0.0024995056 7.602641 1 CCATGTA 3980 0.0 7.520038 2 CCCATGT 3890 0.0 7.060293 1 TACTCTG 4455 0.0 7.0368276 7 >>END_MODULE