FastQCFastQC Report
Wed 25 May 2016
SRR1294884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080509
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75290.1845112950369672No Hit
CCCATGTACTCTGCGTTGATACCAC67770.16608222160519678No Hit
GTATCAACGCAGAGTACTTTTTTTT45970.11265751405033049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG658.0233265E-411.6931753
CGGGGTA600.005885382611.081573512
CTAATCG600.005885919611.0814389
CGATACA700.001491072210.8582164
GTATTAG7350.010.3528421
CCGGACT750.002646978210.1343354
TAGGACC2451.8189894E-129.3070424
GTATACG1151.0966964E-49.0981241
TCCGTGC950.00182762268.9984618
GTCTAGG3150.08.756781
TAGACTG5650.08.7432615
TTAACCT3850.08.6372174
GTGCTAG4600.08.4777971
GTCTAAG2701.4551915E-118.4548221
TGGACCG1356.804105E-58.4443465
GTCTAGT3850.08.399921
CCAGTAC3450.08.2614833
TCTGTCG2303.7125574E-98.2594578
GACAATC2201.4706529E-88.2031427
GGACCGA1050.004522068.1414646
Produced by FastQC (version 0.11.5)