FastQCFastQC Report
Wed 25 May 2016
SRR1294884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080509
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT128100.3139314237513016No Hit
GGTATCAACGCAGAGTACTTTTTTT78660.19277006863604515No Hit
CCCATGTACTCTGCGTTGATACCAC74250.18196259339214788No Hit
GTACATGGGAAGCAGTGGTATCAAC73630.18044317510389024No Hit
TATCAACGCAGAGTACTTTTTTTTT72740.1782620746578429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG855.325167E-511.1769615
AGGCCGT855.325167E-511.1769616
GGATCGT803.7719792E-410.6879686
GTATTAG6600.010.2246561
TTACGGT800.0045164739.5004164
GTGTAGA5800.09.3407371
CTAAGAC4000.09.0253953
GTCCTAC2750.08.98617651
CGGACAG850.0074364058.9415685
GGACCGT850.0074364058.9415686
TATACCG850.0074364058.9415685
TGTCGCC1856.5905624E-88.7291510
TATTCCG1752.6267116E-78.6860955
CTGTCGC1901.0135955E-78.4994359
GTCCTAG4750.08.4040771
GTATCAA70850.08.3442031
TAGGACT5550.08.2165774
TAGGACC2909.094947E-128.1900154
ACACCGA1401.0393269E-48.1432156
TAGACTG5850.08.1200145
Produced by FastQC (version 0.11.5)