FastQCFastQC Report
Wed 25 May 2016
SRR1294883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960853
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80460.20313806142262789No Hit
CCCATGTACTCTGCGTTGATACCAC74670.18851999809132025No Hit
GTATCAACGCAGAGTACTTTTTTTT41850.10565905879364876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG250.00603774918.99778710
AACCGTC550.003073146312.0891967
CTGTCGC2950.010.6256139
ATGCGCC909.536374E-510.5540619
TCTGTCG3200.010.09231958
TACACCG900.00111459939.4997355
CTTACAC5050.09.4057973
ATACCGT1356.8661157E-69.14685256
TATACCG1151.11178175E-49.0867025
GTCTTGC8350.08.7730951
GTATTAC5100.08.7675091
GTCTAGA4600.08.6864031
GTCTCGC2002.480192E-88.5623121
GTGTTAG6300.08.4566051
GTATTAG6200.08.4395551
CTAGGAC3850.08.38948253
TGCGTTG21000.08.36775712
TGTCGCC3750.08.35902710
TCTATAC5850.08.2819253
TTACACT6450.08.2481444
Produced by FastQC (version 0.11.5)