FastQCFastQC Report
Wed 25 May 2016
SRR1294883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3960853
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118540.2992789684444234No Hit
CCCATGTACTCTGCGTTGATACCAC82190.2075058074611706No Hit
GTACATGGGAAGCAGTGGTATCAAC80670.20366825024811575No Hit
GGTATCAACGCAGAGTACTTTTTTT71820.18132457831684234No Hit
TATCAACGCAGAGTACTTTTTTTTT67620.1707208018070855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTC604.0967175E-412.66585910
ACCGGTC550.003071355612.09013758
GTCGTAG600.00585525211.0892061
GACAGCG900.00111496539.4993947
GCGACAG1054.4982228E-49.04704313
GTTCCGG950.00181598319.0047681
GTCTTAC5150.08.859061
AATAGCG1107.1467384E-48.6365775
GTCCTAT3650.08.5935921
CCGCGGC1000.0029081378.54945514
GTAGGAC2159.722498E-98.3957673
ATTATAC7250.08.3866253
ACACCGT1252.744358E-48.3602066
GTCTAGC2303.6634447E-98.2652461
TATACTG8750.08.2516325
ATTAGAC4150.08.2413743
GTACTAA3700.08.220571
CTAGGAC4550.08.1432633
GTATTAA7500.08.1109621
AGCACCG1304.187093E-48.0386595
Produced by FastQC (version 0.11.5)