FastQCFastQC Report
Wed 25 May 2016
SRR1294882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732685
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72060.15226029199069874No Hit
CCCATGTACTCTGCGTTGATACCAC65980.1394134619143256No Hit
GTATCAACGCAGAGTACTTTTTTTT49560.10471856884622577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACT600.00587749711.0836319
ATACCGT951.364984E-510.997626
ACAGCGT1502.6604903E-79.4979438
GTACTAG3150.09.0596471
CTAATAC5700.09.0006213
TGGACCG1856.585287E-88.7296865
ACACCGT1856.607843E-88.727846
AGACCGT1201.7693445E-48.7064496
ACCGTGA1107.1645423E-48.6344948
TGGACTG6550.08.4121715
CGTGTTA1606.4717187E-68.31105210
CCGAATT1150.00111158478.260653514
CACCGTT1150.00111368158.2590827
GTCTTAC4750.08.2109011
TTATACT8250.08.1764094
GGACCGA1050.0045241828.1410946
CCGTCCC1050.0045246138.1410089
CGAGACT1659.743631E-68.0612484
GTTCTAT5200.08.0491471
TCTTATA6500.08.0390172
Produced by FastQC (version 0.11.5)