FastQCFastQC Report
Wed 25 May 2016
SRR1294882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732685
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT149430.31574043064349305No Hit
GGTATCAACGCAGAGTACTTTTTTT89220.18851877950888343No Hit
TATCAACGCAGAGTACTTTTTTTTT85340.1803204734733032No Hit
CCCATGTACTCTGCGTTGATACCAC72900.15403518298809238No Hit
GTACATGGGAAGCAGTGGTATCAAC71370.1508023458142682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1553.6689016E-910.4194945
CGTGTTA1356.458049E-79.851150510
TATACCG1304.2428237E-69.5001275
TTAGACT3950.09.1393624
ACGGATC950.0018260758.9993598
TCTATAC6750.08.8669733
GTATTAG6750.08.731671
GTATCAA78650.08.6783091
CCTAGTA3200.08.6110362
GGTATCA46150.08.4660241
TAGGACT6600.08.3485974
TGCGTTG20550.08.32057612
ACACCGT1951.537901E-78.2821626
GTCCTAG5400.08.2739621
CCTATAC5600.08.1431393
CTGCGTT20950.08.11636711
TAATACT8800.08.09674
ATAGGAC5100.08.010083
GTATAGG4400.07.99389551
CCAGGAC8150.07.9266543
Produced by FastQC (version 0.11.5)