FastQCFastQC Report
Wed 25 May 2016
SRR1294881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4060442
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC78020.19214656926511942No Hit
CCCATGTACTCTGCGTTGATACCAC69440.17101586477531264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG658.024028E-411.6930515
ACCGTCC909.545269E-510.5531048
GGACCGT750.002654251910.13098056
GGTGTCG1151.0302852E-59.9107428
GTCTAGG4800.09.7127821
AGTACGG900.00111367939.5006055
TACACCG1002.7555338E-49.5006055
GTATTAG7000.09.1067951
GTATTAA7900.09.0328131
GTGCCGA850.00745249548.9392111
GTACGGT850.0074532498.93916
ATTAGAC4900.08.9194843
TATACTG8250.08.867235
TAGGACA4650.08.78626254
TGCGTTG19950.08.61715212
ACACCGT1554.2243464E-68.5786526
GTATAAT8100.08.5748521
CCTACGC1000.0029031968.5510713
GTCTTAG4250.08.5071381
TTAGACA5500.08.4649064
Produced by FastQC (version 0.11.5)