FastQCFastQC Report
Wed 25 May 2016
SRR1294881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4060442
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT111080.2735662767747945No Hit
CCCATGTACTCTGCGTTGATACCAC75140.18505374538042904No Hit
GTACATGGGAAGCAGTGGTATCAAC74350.18310814438428133No Hit
GGTATCAACGCAGAGTACTTTTTTT67490.1662134319367202No Hit
TATCAACGCAGAGTACTTTTTTTTT64720.15939151451984784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC751.481405E-512.6654768
TTAGACG701.0908118E-412.2153953
AGACCGT951.36039835E-511.0008636
GGCGTGC803.776775E-410.6864968
AAGACCG909.515265E-510.5563835
GCACCGT1106.0461625E-610.36444956
GTCTTAG3650.09.896551
GTCTACG1106.795705E-59.5058971
GTGCGCC800.00452169.49910711
TGCTCGT850.0074448048.94033610
GTCTTAA4750.08.8054631
GTCTAAT5300.08.788471
GACAGCG1856.593291E-88.72890957
AGCACCG1201.7635335E-48.7090175
TAGACAG7000.08.6863965
GTCTCGC2304.0017767E-108.67929651
ATGCTCG1000.00290896498.5491969
CAATACT4600.08.4680554
GCGTGCA1356.810634E-58.4436519
GTATTAG6550.08.4174361
Produced by FastQC (version 0.11.5)