FastQCFastQC Report
Wed 25 May 2016
SRR1294880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610393
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC171612.811467366106754No Hit
CCCATGTACTCTGCGTTGATACCAC162362.6599256544554084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA150802.470539472110591No Hit
GAGTACATGGGAAGCAGTGGTATCA80751.3229181854968848No Hit
CATGTACTCTGCGTTGATACCACTG63571.0414601740190337No Hit
GCGTTGATACCACTGCTTCCCATGT54040.8853312537987821No Hit
ACGCAGAGTACATGGGAAGCAGTGG50530.8278273178100012No Hit
GGTATCAACGCAGAGTACATGGGAA48950.8019423551711766No Hit
TATCAACGCAGAGTACATGGGAAGC48720.7981742909895756No Hit
GTATCAACGCAGAGTACATGGGAAG48480.7942423979305135No Hit
ACTCTGCGTTGATACCACTGCTTCC44190.7239598094997813No Hit
GTGGTATCAACGCAGAGTACATGGG33380.5468607929645327No Hit
GCTTCCCATGTACTCTGCGTTGATA32460.5317885362381285No Hit
GTACTCTGCGTTGATACCACTGCTT29280.4796909532055577No Hit
ATACCACTGCTTCCCATGTACTCTG23780.3895850706020547No Hit
GATACCACTGCTTCCCATGTACTCT23730.38876592621475015No Hit
GGGAAGCAGTGGTATCAACGCAGAG22000.360423530414012No Hit
CTGCTTCCCATGTACTCTGCGTTGA21870.3582937550070201No Hit
CAGTGGTATCAACGCAGAGTACATG21850.3579660972520982No Hit
GTATCAACGCAGAGTACTTTTTTTT21780.35681929510987187No Hit
CATGGGAAGCAGTGGTATCAACGCA20440.3348662255301093No Hit
GCAGTGGTATCAACGCAGAGTACAT19690.32257905972054074No Hit
GCAGAGTACATGGGAAGCAGTGGTA19210.3147152736024168No Hit
TACCACTGCTTCCCATGTACTCTGC19160.31389612921511223No Hit
GTTGATACCACTGCTTCCCATGTAC18450.3022642789153873No Hit
ACATGGGAAGCAGTGGTATCAACGC17560.2876835088213659No Hit
GGTATCAACGCAGAGTACTTTTTTT17320.28375161576230395No Hit
CCACTGCTTCCCATGTACTCTGCGT16590.2717921077076572No Hit
CTTCCCATGTACTCTGCGTTGATAC16110.2639283215895333No Hit
CCATGTACTCTGCGTTGATACCACT15720.2575389953685576No Hit
AAGCAGTGGTATCAACGCAGAGTAC14430.23640507017609966No Hit
TATCAACGCAGAGTACTTTTTTTTT13720.22477321987637472No Hit
ATGGGAAGCAGTGGTATCAACGCAG13600.22280727334684378No Hit
TGATACCACTGCTTCCCATGTACTC12150.19905208611501116No Hit
TTCCCATGTACTCTGCGTTGATACC11490.1882393802025908No Hit
ATCAACGCAGAGTACATGGGAAGCA11130.18234154061399788No Hit
GTACATGGTAAGCAGTGGTATCAAC9620.15760338011739977No Hit
GGTATCAACGCAGAGTACATGGGGG8660.14187580788115198No Hit
GGAAGCAGTGGTATCAACGCAGAGT7480.12254400034076407No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7470.12238017146330314No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7120.11664616075217114No Hit
ACCATGTACTCTGCGTTGATACCAC6910.11320575432549194No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6210.10173773290322792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG250.00602691919.00057415
TGAACTG307.718066E-418.9990165
CGCGTGA350.002168917316.28487214
GTAAAAA1153.6925485E-1013.2297211
CCTGTAA604.0536752E-412.6784831
ACTGAGT550.003069728812.0883018
TGCACTG752.0708755E-411.399415
AGCACAG951.3571127E-510.9994315
CCTGCAC750.002642967310.1336393
AAGAGAG750.002644765710.1328095
TAAATGT856.5788877E-410.0591284
TTTTATA856.5788877E-410.0591282
GTCCTGG1252.5291247E-69.8892161
AAAAGTA1002.7478443E-49.5002872
CCACCCT1054.4801613E-49.0478934
CCAGGAC850.00742213668.9414473
ATGGGTG1201.75954E-48.7078835
GTACAAA1107.060884E-48.644421
TGGGTAA1554.198946E-68.57879456
TCCTGGT1100.00683807957.77296162
Produced by FastQC (version 0.11.5)