FastQCFastQC Report
Wed 25 May 2016
SRR1294880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610393
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC179102.934175195324979No Hit
GTACATGGGAAGCAGTGGTATCAAC171442.8086822751899185No Hit
GTATCAACGCAGAGTACTTTTTTTT90951.4900236405070175No Hit
GAGTACATGGGAAGCAGTGGTATCA83481.3676434690437145No Hit
GGTATCAACGCAGAGTACTTTTTTT74891.226914463304789No Hit
CATGTACTCTGCGTTGATACCACTG66071.0824173933842622No Hit
TATCAACGCAGAGTACTTTTTTTTT58860.964296772734943No Hit
GCGTTGATACCACTGCTTCCCATGT57670.9448011363170941No Hit
ACGCAGAGTACATGGGAAGCAGTGG56760.9298927084681509No Hit
TATCAACGCAGAGTACATGGGAAGC51070.8366740771928906No Hit
GTATCAACGCAGAGTACATGGGAAG47680.7811360877336405No Hit
GGTATCAACGCAGAGTACATGGGAA47630.7803169433463358No Hit
ACTCTGCGTTGATACCACTGCTTCC45160.73985121061349No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33670.5516118304108992No Hit
GCTTCCCATGTACTCTGCGTTGATA33350.54636930633215No Hit
GTGGTATCAACGCAGAGTACATGGG32950.5398161512337134No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28780.471499509332512No Hit
GTACTCTGCGTTGATACCACTGCTT26870.4402081937374774No Hit
ATACCACTGCTTCCCATGTACTCTG26030.4264465680307605No Hit
GATACCACTGCTTCCCATGTACTCT24820.4066232738579898No Hit
GGGAAGCAGTGGTATCAACGCAGAG24040.39384462141603854No Hit
CAGTGGTATCAACGCAGAGTACATG23400.38335957325854No Hit
CATGGGAAGCAGTGGTATCAACGCA22680.37156389408135415No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21910.35894907051686376No Hit
CTGCTTCCCATGTACTCTGCGTTGA21240.34797253572698245No Hit
GCAGAGTACATGGGAAGCAGTGGTA20470.335357712162492No Hit
GCAGTGGTATCAACGCAGAGTACAT20230.3314258191034301No Hit
ACATGGGAAGCAGTGGTATCAACGC19820.3247088351275326No Hit
TACCACTGCTTCCCATGTACTCTGC18740.30701531636175383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17900.293253690655037No Hit
CTTCCCATGTACTCTGCGTTGATAC17850.29243454626773246No Hit
CCACTGCTTCCCATGTACTCTGCGT17690.2898132842283578No Hit
GTTGATACCACTGCTTCCCATGTAC17370.2845707601496085No Hit
CCATGTACTCTGCGTTGATACCACT16730.27408571199211No Hit
ATGGGAAGCAGTGGTATCAACGCAG15310.25082201139266014No Hit
AAGCAGTGGTATCAACGCAGAGTAC15190.24885606486312917No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13140.21527114498364167No Hit
TTCCCATGTACTCTGCGTTGATACC12370.20265632141915124No Hit
GTGGTATCAACGCAGAGTACTTTTT12350.20232866366422944No Hit
TGATACCACTGCTTCCCATGTACTC12110.19839677060516747No Hit
ATCAACGCAGAGTACATGGGAAGCA12050.19741379734040201No Hit
GTACATGGTAAGCAGTGGTATCAAC9850.1613714442990008No Hit
ATCAACGCAGAGTACTTTTTTTTTT9580.15694806460755611No Hit
GGTATCAACGCAGAGTACATGGGGG8770.14367792553322203No Hit
GGAAGCAGTGGTATCAACGCAGAGT8490.13909071696431644No Hit
ACCATGTACTCTGCGTTGATACCAC8030.13155458860111435No Hit
CTGCGTTGATACCACTGCTTCCCAT6710.10992917677627365No Hit
TCAACGCAGAGTACATGGGAAGCAG6360.10419516606514163No Hit
CCCCATGTACTCTGCGTTGATACCA6190.10141007514830609No Hit
GGTATCAACGCAGAGTACATGGGGA6190.10141007514830609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGT307.7102426E-419.0018844
GACAGTA400.00527650814.2490787
ACCGGTT400.00527650814.24907812
CTAGAAC500.001496609413.3013193
GGACTGT500.001496609413.3013196
AAAGAGT500.001496609413.3013196
CCCCCGG550.00306626112.09012818
TACAGTG855.3138603E-511.1757477
GTGCATA600.0058715611.08261611
CGGTTCT600.0058715611.08261614
ATGGCCA700.001487923710.858224
GGGTAGA803.7516252E-410.6911881
GTACAGT750.002641458510.1343386
CCAAGAC750.002641458510.1343383
CCTGCAC750.002641458510.1343383
TGACCGG750.002645052510.13267810
ACAGTGC856.584648E-410.0581728
GAATCAT800.0044960039.5032781
GTGTAGG800.0044960039.5032781
TGGACTG800.0045051329.5009425
Produced by FastQC (version 0.11.5)