##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294880_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 610393 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71343052754537 33.0 31.0 34.0 30.0 34.0 2 31.916948588859963 33.0 31.0 34.0 30.0 34.0 3 32.002604879151626 33.0 31.0 34.0 30.0 34.0 4 35.46540671337974 37.0 35.0 37.0 33.0 37.0 5 35.68465890008568 37.0 35.0 37.0 35.0 37.0 6 35.451877724679015 37.0 35.0 37.0 33.0 37.0 7 35.41971483945589 37.0 35.0 37.0 33.0 37.0 8 35.44677117856856 37.0 35.0 37.0 33.0 37.0 9 37.092992547424366 39.0 37.0 39.0 34.0 39.0 10 37.122473553923456 39.0 37.0 39.0 34.0 39.0 11 37.13693309064816 39.0 37.0 39.0 34.0 39.0 12 37.14601084874827 39.0 37.0 39.0 34.0 39.0 13 37.02416312113671 39.0 37.0 39.0 33.0 39.0 14 38.32862926016517 40.0 38.0 41.0 33.0 41.0 15 38.266089224483245 40.0 38.0 41.0 33.0 41.0 16 38.1880902959241 40.0 38.0 41.0 33.0 41.0 17 38.29997722778603 40.0 38.0 41.0 33.0 41.0 18 38.30143530479543 40.0 38.0 41.0 33.0 41.0 19 38.32161574592107 40.0 38.0 41.0 34.0 41.0 20 38.33935349848377 40.0 38.0 41.0 34.0 41.0 21 38.294859213654156 40.0 38.0 41.0 33.0 41.0 22 38.35141621873121 40.0 38.0 41.0 34.0 41.0 23 38.28558813747864 40.0 38.0 41.0 33.0 41.0 24 38.29217242006379 40.0 38.0 41.0 33.0 41.0 25 37.22594459635022 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12043953562914922 1101 2 -0.032066687191186105 1101 3 -0.08621609254487339 1101 4 -0.08642290576217704 1101 5 0.034701345157131414 1101 6 0.09873004681187325 1101 7 0.21957003412979503 1101 8 0.05966142850867584 1101 9 0.14277851147484455 1101 10 0.21522097514429817 1101 11 0.1020355410778393 1101 12 0.062014188564660344 1101 13 0.17312459955221016 1101 14 0.2658424445525327 1101 15 0.19750385211780497 1101 16 0.27198084578393633 1101 17 0.34225057589875263 1101 18 0.22105264193068308 1101 19 0.16551265596325493 1101 20 0.28823663603529326 1101 21 0.23960135882437328 1101 22 0.24687386162123914 1101 23 0.32502624421148596 1101 24 0.11310174290101571 1101 25 0.08799232851637129 1102 1 0.011251214410521015 1102 2 0.12750037514917167 1102 3 0.16691269847491697 1102 4 0.01679776550106027 1102 5 -0.010314855454041094 1102 6 0.11651744770149719 1102 7 0.0891435971893344 1102 8 0.13637503343898771 1102 9 0.22065405597405885 1102 10 0.24412870076276505 1102 11 0.2961639072474824 1102 12 0.10612694277093482 1102 13 0.19018514837670608 1102 14 0.3465281424584816 1102 15 0.45374223912045863 1102 16 0.38383672432797766 1102 17 0.3009530937757319 1102 18 0.33267306135503816 1102 19 0.39008691004411844 1102 20 0.40951855738170906 1102 21 0.24390104709696914 1102 22 0.3716595170421684 1102 23 0.3684863671620988 1102 24 0.22300228701632818 1102 25 0.319059623756516 1103 1 -0.004409846411171259 1103 2 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8 0.2244135496901265 2210 9 0.270532553226154 2210 10 0.26785711993164796 2210 11 0.25316896646358344 2210 12 0.23681496257600543 2210 13 0.2538427529134495 2210 14 0.1488828729094962 2210 15 0.3249229387747832 2210 16 0.3719155415892885 2210 17 0.21345552263125 2210 18 0.16681401413808317 2210 19 0.3271477669683378 2210 20 0.2919271664451202 2210 21 0.16807157794283256 2210 22 0.3021462716833838 2210 23 0.34225112920621825 2210 24 0.20235281704658092 2210 25 0.3942653868009671 2211 1 0.061306496116884546 2211 2 0.1844866007906738 2211 3 -0.005943983701335753 2211 4 0.15654761577977894 2211 5 0.08402107304829087 2211 6 0.057006690802801074 2211 7 0.219910170184221 2211 8 0.08437798179665634 2211 9 0.19833406703040168 2211 10 0.06726179147082689 2211 11 0.13208089255176247 2211 12 0.16360149015196157 2211 13 0.12142391928010454 2211 14 0.18874493888360178 2211 15 0.06451065483889096 2211 16 0.1674456983916457 2211 17 0.26526644891462325 2211 18 0.24395513626174647 2211 19 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0.3949854588025872 2213 6 0.34746287327579495 2213 7 0.13501621260118668 2213 8 0.26839158723883827 2213 9 0.22456455922728225 2213 10 0.11730180747723296 2213 11 0.2818608045165476 2213 12 0.15847944133243885 2213 13 0.023604791629040278 2213 14 0.18086178562229804 2213 15 0.13273794575525955 2213 16 0.2196265707405871 2213 17 0.22230926604147783 2213 18 0.1557598581505033 2213 19 0.09792562114932934 2213 20 0.18649593975678158 2213 21 0.13119799747983052 2213 22 0.24670045892682424 2213 23 0.22497288953628214 2213 24 0.1099938330704191 2213 25 0.18018253793347583 2214 1 0.2670227632062421 2214 2 0.17854247556777025 2214 3 0.2750150018346247 2214 4 0.20365480817129367 2214 5 0.24273912337521608 2214 6 0.3900956545436358 2214 7 0.2859394945353202 2214 8 0.21871521183430787 2214 9 0.45628928795372303 2214 10 0.3420686074010817 2214 11 0.3349637932848708 2214 12 0.3766785656423579 2214 13 0.2665992709741758 2214 14 0.43966627829315286 2214 15 0.4819764761188594 2214 16 0.39805794328960786 2214 17 0.37155731477552933 2214 18 0.2357522434541366 2214 19 0.26647692516608146 2214 20 0.2851919230044544 2214 21 0.3269337675160031 2214 22 0.1485470227950927 2214 23 0.3121230894220588 2214 24 0.20413051687015127 2214 25 0.05580599119827667 2215 1 0.1642087966382313 2215 2 0.12471568387689658 2215 3 0.10431824084660235 2215 4 0.20976679518943087 2215 5 0.17116276733283797 2215 6 0.2594370646617463 2215 7 0.23443523947119616 2215 8 0.218391587238834 2215 9 0.17046422963675667 2215 10 0.05156804958661354 2215 11 0.21234234186083256 2215 12 0.14827889387698434 2215 13 0.1524160312245968 2215 14 0.09451465417712512 2215 15 0.016747218980221135 2215 16 0.008884154115818887 2215 17 -0.05822813410377137 2215 18 0.06724525002805137 2215 19 0.046174322339211926 2215 20 0.13271402813173694 2215 21 0.28932324187958613 2215 22 0.23613680326513276 2215 23 0.12974357273833448 2215 24 0.2606781523821837 2215 25 0.37148749296391514 2216 1 0.34209246812963556 2216 2 0.13406755571707762 2216 3 0.14645096866689045 2216 4 0.410959520859862 2216 5 0.3332574373788688 2216 6 0.37587040092670065 2216 7 0.3846044295530717 2216 8 0.24208974751323353 2216 9 0.16442864330805662 2216 10 0.15078921553681113 2216 11 0.29580915279879605 2216 12 0.2988451853849696 2216 13 0.28879949439321706 2216 14 0.23641489554727713 2216 15 0.15145343943635936 2216 16 0.14978853262543623 2216 17 0.0709106710562395 2216 18 0.2235068821238002 2216 19 0.36123565985143813 2216 20 0.30138707862580105 2216 21 0.17748277893217335 2216 22 0.2692632352066511 2216 23 0.24213571350590968 2216 24 0.32160079578066814 2216 25 0.2389959791191174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 24.0 9 76.0 10 148.0 11 214.0 12 289.0 13 485.0 14 735.0 15 1183.0 16 1609.0 17 1880.0 18 2127.0 19 2189.0 20 2240.0 21 2122.0 22 2102.0 23 2155.0 24 2308.0 25 2371.0 26 2551.0 27 2759.0 28 3133.0 29 3883.0 30 4962.0 31 6809.0 32 9423.0 33 13290.0 34 19442.0 35 30137.0 36 49993.0 37 99948.0 38 339805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05297660507232 18.25214077928463 12.75568484451182 23.939197771131234 2 15.270870577593962 22.466830418517613 34.55715720669274 27.705141797195683 3 20.930697384092888 27.466615114166398 28.181578391201562 23.421109110539152 4 14.106063336555316 20.075361654024476 30.939399400383362 34.87917560903684 5 13.070759330464144 37.80744536716509 33.51627557983136 15.605519722539412 6 32.310928913400375 32.17657448642201 17.329099530414894 18.183397069762716 7 24.68933949111474 33.91290529216423 21.66374778216657 19.73400743455446 8 28.16464146869312 31.95564169313868 20.45452683762756 19.425190000540635 9 27.409226514720842 15.74805084593041 19.88653211947057 36.95619051987818 10 19.02331776412901 29.35076254151014 29.696932959585055 21.928986734775794 11 30.015907784001456 25.04402901081762 19.638659027872208 25.30140417730872 12 24.76945181219313 29.28129909746671 27.343694963736475 18.60555412660368 13 30.456607464371316 20.95404108500589 23.830057028832243 24.75929442179055 14 23.339717198591728 24.92574456129084 25.764056927258338 25.970481312859096 15 31.02722344456768 23.41180190467453 23.33316404349329 22.2278106072645 16 20.355082709008787 26.924456866313996 30.166630351265493 22.55383007341172 17 24.409683597288957 28.11631194984215 26.81780426708694 20.656200185781948 18 24.528951020080505 23.273202674342595 30.001818500539816 22.196027805037083 19 23.353151166543523 27.537013039140355 31.00330442845839 18.106531365857734 20 24.349722228138265 23.713738525834994 29.7126605318213 22.22387871420544 21 21.746481365284335 25.88070308801051 31.179584300606333 21.193231246098826 22 23.568133265180407 25.207407698659225 26.04129805108848 25.18316098507189 23 20.665702260674678 25.22850032683861 30.080620190598516 24.025177221888192 24 22.76287572105185 29.227727054536995 27.201000011468025 20.808397212943134 25 22.977819208280568 23.249938973743145 26.907746320812986 26.864495497163304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2284.0 1 2284.0 2 1916.5 3 1549.0 4 1549.0 5 1549.0 6 2919.5 7 4290.0 8 4290.0 9 4290.0 10 4168.5 11 4047.0 12 4047.0 13 4047.0 14 3415.0 15 2783.0 16 2783.0 17 2783.0 18 4507.5 19 6232.0 20 6232.0 21 6232.0 22 9422.5 23 12613.0 24 12613.0 25 12613.0 26 17106.5 27 21600.0 28 21600.0 29 21600.0 30 25738.5 31 29877.0 32 29877.0 33 29877.0 34 32727.5 35 35578.0 36 35578.0 37 35578.0 38 36586.5 39 37595.0 40 37595.0 41 37595.0 42 44675.5 43 51756.0 44 51756.0 45 51756.0 46 91724.5 47 131693.0 48 131693.0 49 131693.0 50 118004.0 51 104315.0 52 104315.0 53 104315.0 54 82555.5 55 60796.0 56 60796.0 57 60796.0 58 49333.5 59 37871.0 60 37871.0 61 37871.0 62 33347.0 63 28823.0 64 28823.0 65 28823.0 66 23552.0 67 18281.0 68 18281.0 69 18281.0 70 14131.0 71 9981.0 72 9981.0 73 9981.0 74 7537.5 75 5094.0 76 5094.0 77 5094.0 78 3610.0 79 2126.0 80 2126.0 81 2126.0 82 1495.5 83 865.0 84 865.0 85 865.0 86 540.5 87 216.0 88 216.0 89 216.0 90 150.5 91 85.0 92 85.0 93 85.0 94 57.5 95 30.0 96 30.0 97 30.0 98 21.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035714695286479366 2 0.019659465295309743 3 0.0019659465295309743 4 4.914866323827436E-4 5 0.0 6 0.010976534789881273 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.6382887746091453E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 610393.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.82946658798772 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45032715266447 27.35548624287189 2 15.561494182203866 12.084890368141004 3 6.097555589152822 7.102944930522217 4 2.859336506480956 4.441060453688633 5 1.5778861999389147 3.063423974008749 6 0.8804200675184464 2.051174495706084 7 0.5971078350703578 1.6229765111903094 8 0.4005732237861523 1.244323568723754 9 0.2862957001029057 1.0005038389287295 >10 1.1826764788327429 7.724936993899897 >50 0.0489442041882681 1.288392569817835 >100 0.03459848915386404 3.1171105068469545 >500 0.0054851263292711285 1.5284097507818375 >1k 0.013079916631338843 11.788366041162563 >5k 0.00337546235647454 8.842953831970165 >10k+ 8.43865589118635E-4 5.743045921739364 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 17910 2.934175195324979 No Hit GTACATGGGAAGCAGTGGTATCAAC 17144 2.8086822751899185 No Hit GTATCAACGCAGAGTACTTTTTTTT 9095 1.4900236405070175 No Hit GAGTACATGGGAAGCAGTGGTATCA 8348 1.3676434690437145 No Hit GGTATCAACGCAGAGTACTTTTTTT 7489 1.226914463304789 No Hit CATGTACTCTGCGTTGATACCACTG 6607 1.0824173933842622 No Hit TATCAACGCAGAGTACTTTTTTTTT 5886 0.964296772734943 No Hit GCGTTGATACCACTGCTTCCCATGT 5767 0.9448011363170941 No Hit ACGCAGAGTACATGGGAAGCAGTGG 5676 0.9298927084681509 No Hit TATCAACGCAGAGTACATGGGAAGC 5107 0.8366740771928906 No Hit GTATCAACGCAGAGTACATGGGAAG 4768 0.7811360877336405 No Hit GGTATCAACGCAGAGTACATGGGAA 4763 0.7803169433463358 No Hit ACTCTGCGTTGATACCACTGCTTCC 4516 0.73985121061349 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3367 0.5516118304108992 No Hit GCTTCCCATGTACTCTGCGTTGATA 3335 0.54636930633215 No Hit GTGGTATCAACGCAGAGTACATGGG 3295 0.5398161512337134 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2878 0.471499509332512 No Hit GTACTCTGCGTTGATACCACTGCTT 2687 0.4402081937374774 No Hit ATACCACTGCTTCCCATGTACTCTG 2603 0.4264465680307605 No Hit GATACCACTGCTTCCCATGTACTCT 2482 0.4066232738579898 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2404 0.39384462141603854 No Hit CAGTGGTATCAACGCAGAGTACATG 2340 0.38335957325854 No Hit CATGGGAAGCAGTGGTATCAACGCA 2268 0.37156389408135415 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2191 0.35894907051686376 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2124 0.34797253572698245 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2047 0.335357712162492 No Hit GCAGTGGTATCAACGCAGAGTACAT 2023 0.3314258191034301 No Hit ACATGGGAAGCAGTGGTATCAACGC 1982 0.3247088351275326 No Hit TACCACTGCTTCCCATGTACTCTGC 1874 0.30701531636175383 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1790 0.293253690655037 No Hit CTTCCCATGTACTCTGCGTTGATAC 1785 0.29243454626773246 No Hit CCACTGCTTCCCATGTACTCTGCGT 1769 0.2898132842283578 No Hit GTTGATACCACTGCTTCCCATGTAC 1737 0.2845707601496085 No Hit CCATGTACTCTGCGTTGATACCACT 1673 0.27408571199211 No Hit ATGGGAAGCAGTGGTATCAACGCAG 1531 0.25082201139266014 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1519 0.24885606486312917 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1314 0.21527114498364167 No Hit TTCCCATGTACTCTGCGTTGATACC 1237 0.20265632141915124 No Hit GTGGTATCAACGCAGAGTACTTTTT 1235 0.20232866366422944 No Hit TGATACCACTGCTTCCCATGTACTC 1211 0.19839677060516747 No Hit ATCAACGCAGAGTACATGGGAAGCA 1205 0.19741379734040201 No Hit GTACATGGTAAGCAGTGGTATCAAC 985 0.1613714442990008 No Hit ATCAACGCAGAGTACTTTTTTTTTT 958 0.15694806460755611 No Hit GGTATCAACGCAGAGTACATGGGGG 877 0.14367792553322203 No Hit GGAAGCAGTGGTATCAACGCAGAGT 849 0.13909071696431644 No Hit ACCATGTACTCTGCGTTGATACCAC 803 0.13155458860111435 No Hit CTGCGTTGATACCACTGCTTCCCAT 671 0.10992917677627365 No Hit TCAACGCAGAGTACATGGGAAGCAG 636 0.10419516606514163 No Hit CCCCATGTACTCTGCGTTGATACCA 619 0.10141007514830609 No Hit GGTATCAACGCAGAGTACATGGGGA 619 0.10141007514830609 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGAGT 30 7.7102426E-4 19.001884 4 GACAGTA 40 0.005276508 14.249078 7 ACCGGTT 40 0.005276508 14.249078 12 CTAGAAC 50 0.0014966094 13.301319 3 GGACTGT 50 0.0014966094 13.301319 6 AAAGAGT 50 0.0014966094 13.301319 6 CCCCCGG 55 0.003066261 12.090128 18 TACAGTG 85 5.3138603E-5 11.175747 7 GTGCATA 60 0.00587156 11.082616 11 CGGTTCT 60 0.00587156 11.082616 14 ATGGCCA 70 0.0014879237 10.85822 4 GGGTAGA 80 3.7516252E-4 10.691188 1 GTACAGT 75 0.0026414585 10.134338 6 CCAAGAC 75 0.0026414585 10.134338 3 CCTGCAC 75 0.0026414585 10.134338 3 TGACCGG 75 0.0026450525 10.132678 10 ACAGTGC 85 6.584648E-4 10.058172 8 GAATCAT 80 0.004496003 9.503278 1 GTGTAGG 80 0.004496003 9.503278 1 TGGACTG 80 0.004505132 9.500942 5 >>END_MODULE