FastQCFastQC Report
Wed 25 May 2016
SRR1294879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171380
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC118596.919710584665656No Hit
CCCATGTACTCTGCGTTGATACCAC113346.613373789240285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69974.082740109697747No Hit
GAGTACATGGGAAGCAGTGGTATCA53663.131053798576263No Hit
CATGTACTCTGCGTTGATACCACTG44362.588400046679893No Hit
GCGTTGATACCACTGCTTCCCATGT35942.0970941766833935No Hit
TATCAACGCAGAGTACATGGGAAGC33801.972225463881433No Hit
ACGCAGAGTACATGGGAAGCAGTGG32931.9214610806395145No Hit
GTATCAACGCAGAGTACATGGGAAG32451.8934531450577663No Hit
GGTATCAACGCAGAGTACATGGGAA29971.7487454778854008No Hit
ACTCTGCGTTGATACCACTGCTTCC28891.6857276228264675No Hit
GCTTCCCATGTACTCTGCGTTGATA21011.2259306803594352No Hit
GTACTCTGCGTTGATACCACTGCTT19111.1150659353483487No Hit
GTGGTATCAACGCAGAGTACATGGG17441.0176216594701832No Hit
ATACCACTGCTTCCCATGTACTCTG16820.9814447426770918No Hit
GGGAAGCAGTGGTATCAACGCAGAG15770.9201773835920177No Hit
GATACCACTGCTTCCCATGTACTCT14890.8688295016921461No Hit
CTGCTTCCCATGTACTCTGCGTTGA14390.8396545687944919No Hit
CAGTGGTATCAACGCAGAGTACATG14010.8174816197922745No Hit
TACCACTGCTTCCCATGTACTCTGC13270.774302719103746No Hit
GCAGAGTACATGGGAAGCAGTGGTA13170.7684677325242152No Hit
CATGGGAAGCAGTGGTATCAACGCA13150.767300735208309No Hit
GCAGTGGTATCAACGCAGAGTACAT13150.767300735208309No Hit
GTTGATACCACTGCTTCCCATGTAC11560.6745244485937683No Hit
CCATGTACTCTGCGTTGATACCACT11100.6476835103279263No Hit
CCACTGCTTCCCATGTACTCTGCGT11020.6430155210643016No Hit
CTTCCCATGTACTCTGCGTTGATAC11020.6430155210643016No Hit
ACATGGGAAGCAGTGGTATCAACGC10230.5969191270860077No Hit
AAGCAGTGGTATCAACGCAGAGTAC9120.532150776053215No Hit
ATGGGAAGCAGTGGTATCAACGCAG8520.4971408565760299No Hit
TGATACCACTGCTTCCCATGTACTC7830.45687944917726686No Hit
GTACATGGTAAGCAGTGGTATCAAC7750.4522114599136422No Hit
TTCCCATGTACTCTGCGTTGATACC7630.4452094760182051No Hit
GTATCAACGCAGAGTACTTTTTTTT7420.4329560042011903No Hit
ATCAACGCAGAGTACATGGGAAGCA7130.4160345431205508No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7050.4113665538569261No Hit
ACCATGTACTCTGCGTTGATACCAC5920.3454312055082273No Hit
GGTATCAACGCAGAGTACTTTTTTT4970.2899988330026841No Hit
GGAAGCAGTGGTATCAACGCAGAGT4900.28591434239701247No Hit
TATCAACGCAGAGTACTTTTTTTTT4290.2503209242618742No Hit
AAAAAGTACTCTGCGTTGATACCAC4280.24973742560392112No Hit
CTGCGTTGATACCACTGCTTCCCAT3890.22698097794375074No Hit
CCCCATGTACTCTGCGTTGATACCA3860.22523048196989148No Hit
TCCATGTACTCTGCGTTGATACCAC3630.21181001283697046No Hit
GGTAAGCAGTGGTATCAACGCAGAG3610.21064301552106432No Hit
TGGGAAGCAGTGGTATCAACGCAGA3580.20889251954720506No Hit
AAACAAAAAAAAAAAAAAAAAAAAA3510.20480802894153344No Hit
CTCTGCGTTGATACCACTGCTTCCC3450.20130703699381491No Hit
TCAACGCAGAGTACATGGGAAGCAG3390.1978060450460964No Hit
TCCCATGTACTCTGCGTTGATACCA2930.17096510678025442No Hit
AAAGTACTCTGCGTTGATACCACTG2890.16863111214844206No Hit
AGTGGTATCAACGCAGAGTACATGG2750.16046213093709885No Hit
GAGTACATGGTAAGCAGTGGTATCA2640.1540436456996149No Hit
GTACATGGAAAGCAGTGGTATCAAC2610.15229314972575564No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2600.15170965106780254No Hit
GTACATGGGTAAGCAGTGGTATCAA2590.15112615240984945No Hit
ATGTACTCTGCGTTGATACCACTGC2550.14879215777803712No Hit
ACTGCTTCCCATGTACTCTGCGTTG2500.1458746644882717No Hit
GAACAAAAAAAAAAAAAAAAAAAAA2490.1452911658303186No Hit
TTGATACCACTGCTTCCCATGTACT2420.14120667522464697No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA2320.13537168864511612No Hit
AACGCAGAGTACATGGGAAGCAGTG2280.13303769401330376No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2140.12486871280196056No Hit
GCTTACCATGTACTCTGCGTTGATA2090.12195121951219512No Hit
GAGTACATGGAAAGCAGTGGTATCA2060.12020072353833586No Hit
ACTCTGCGTTGATACCACTGCTTAC2010.11728323024857042No Hit
CGTTGATACCACTGCTTCCCATGTA1990.11611623293266427No Hit
CAACGCAGAGTACATGGGAAGCAGT1910.11144824366903956No Hit
GCGTTGATACCACTGCTTACCATGT1780.10386276111564945No Hit
ACGCAGAGTACATGGTAAGCAGTGG1750.10211226514179018No Hit
CGCAGAGTACATGGGAAGCAGTGGT1720.10036176916793091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA1200.015.8464791
TACAAAA653.3316046E-614.6147112
GCTGGGA400.005255378314.2493449
GAGAAAA600.005803568811.0925361
CATGGGT1054.4418767E-49.0472024
GAAAAAA5350.08.7081591
AGTACTC1000.00287857958.5496053
GGAAAAA1356.621691E-58.4514561
GAAGCAG30750.07.8776859
GGAAGCA31350.07.72691638
AGTGGTA33300.07.6738214
CAGTGGT33450.07.639408613
AGCAGTG34600.07.440408711
AAGCAGT34650.07.429672210
AGTACTT2958.221832E-107.406438413
TGGTATC34500.07.351835316
GTGGTAT34550.07.341195615
CATGGGG1701.2054774E-47.26437044
GGGAAGC33400.07.252667
GCAGTGG35550.07.241578612
Produced by FastQC (version 0.11.5)