##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294879_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 171380 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.814850040844906 31.0 30.0 34.0 22.0 34.0 2 29.899702415684445 31.0 30.0 34.0 23.0 34.0 3 30.369955654101997 31.0 31.0 34.0 25.0 34.0 4 33.371717820049014 37.0 35.0 37.0 25.0 37.0 5 34.2373147391761 37.0 35.0 37.0 30.0 37.0 6 33.99599719920644 37.0 35.0 37.0 27.0 37.0 7 34.18818415217645 37.0 35.0 37.0 29.0 37.0 8 34.197998599603224 37.0 35.0 37.0 28.0 37.0 9 35.476584198856344 39.0 35.0 39.0 27.0 39.0 10 35.6763683043529 39.0 37.0 39.0 28.0 39.0 11 35.71681643132221 39.0 37.0 39.0 28.0 39.0 12 35.876525848990546 39.0 37.0 39.0 29.0 39.0 13 35.46925545571245 39.0 35.0 39.0 27.0 39.0 14 36.73573345781305 40.0 37.0 41.0 27.0 41.0 15 36.64765433539503 40.0 37.0 41.0 27.0 41.0 16 36.46803594351733 40.0 36.0 41.0 27.0 41.0 17 36.57786206091726 40.0 37.0 41.0 27.0 41.0 18 36.44497024156844 40.0 36.0 41.0 27.0 41.0 19 36.29687244719337 40.0 36.0 41.0 27.0 41.0 20 36.39702415684444 40.0 36.0 41.0 27.0 41.0 21 36.28428638114132 40.0 36.0 41.0 27.0 41.0 22 36.39639981328043 40.0 36.0 41.0 27.0 41.0 23 36.37239467849224 40.0 36.0 41.0 25.0 41.0 24 36.48622943167231 40.0 36.0 41.0 26.0 41.0 25 35.46136655385693 40.0 34.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6986960541494227 1101 2 0.7766251455055944 1101 3 0.6648800584222521 1101 4 1.4246408926637883 1101 5 0.3411359275118784 1101 6 0.5602611219617941 1101 7 0.7163569904030851 1101 8 0.8057702911752287 1101 9 0.7057077329256458 1101 10 0.692666071814763 1101 11 0.7417996211490561 1101 12 0.39825186405330726 1101 13 0.86786632944915 1101 14 0.8631676574973568 1101 15 0.8369056590082167 1101 16 1.0928121241462563 1101 17 0.9471626337166299 1101 18 1.3506757547167467 1101 19 0.5138717204845591 1101 20 0.6496230475601266 1101 21 0.5867895708584285 1101 22 0.5791410676853204 1101 23 0.48999724253059895 1101 24 0.6478270014074283 1101 25 1.2120637650177883 1102 1 -0.9612812701136164 1102 2 -0.7900415211610721 1102 3 -1.0735553157274076 1102 4 -1.1995087672001574 1102 5 -1.6784785849597768 1102 6 -1.292573345158381 1102 7 -0.4911260027941964 1102 8 -0.784932656670577 1102 9 -1.0448591604983903 1102 10 -0.9444087581172127 1102 11 -1.1354112632046878 1102 12 -0.910931809416077 1102 13 -1.0006143961744272 1102 14 -0.7996441338858631 1102 15 -0.7999424135541418 1102 16 -1.0060540890056657 1102 17 -0.8724518787550224 1102 18 -1.2749478280496902 1102 19 -1.4328402976560284 1102 20 -1.34221368713375 1102 21 -1.7263623565792159 1102 22 -1.3045324017024384 1102 23 -1.013744254068044 1102 24 -1.1292705042615054 1102 25 -1.1320405433722343 1103 1 -0.08011684505606098 1103 2 0.09091085979130753 1103 3 -0.248128198315559 1103 4 0.2649586991757076 1103 5 -0.2831980809006467 1103 6 -0.4187698797518635 1103 7 -0.0022128802932854796 1103 8 -0.21580627838388722 1103 9 -0.20183238235710377 1103 10 -0.479759539651198 1103 11 -0.39722514906437567 1103 12 0.11714888851194871 1103 13 0.4308044818093322 1103 14 0.23040554501877608 1103 15 0.15574036846451378 1103 16 0.09851394686008064 1103 17 0.2692844595461992 1103 18 -0.03000659455884147 1103 19 0.15688465084754455 1103 20 -0.0826717024009227 1103 21 0.17318932159841438 1103 22 0.06297032457891305 1103 23 -0.08417318900079351 1103 24 -0.16138004018783647 1103 25 0.2637852169573378 1104 1 -0.5722939947627061 1104 2 -0.44627922285332033 1104 3 -0.4217450048261888 1104 4 -0.5659983382351825 1104 5 -0.8460963207593295 1104 6 -0.24685643580511396 1104 7 -0.16920549905550786 1104 8 -0.2027471653941717 1104 9 -0.42449972195713315 1104 10 -0.8276240276961175 1104 11 -0.5833648247946144 1104 12 -0.4991844658505542 1104 13 -0.6040513632477627 1104 14 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1107 11 0.32033210284402003 1107 12 0.5904313312600422 1107 13 0.4537052451681163 1107 14 0.4250620335683948 1107 15 0.5794044906019167 1107 16 0.579429977394426 1107 17 0.9257730091645229 1107 18 0.8020544741434534 1107 19 0.43551060504601935 1107 20 0.5547328777517464 1107 21 0.4861664208350547 1107 22 0.8339626909911289 1107 23 0.5341474216862281 1107 24 0.17449782240758083 1107 25 0.11874704901840971 1108 1 0.097885842424283 1108 2 -0.38474457092030434 1108 3 0.1657437746952759 1108 4 0.32871488181000785 1108 5 0.1074586363707084 1108 6 -0.0026678698596498407 1108 7 0.24536409886444233 1108 8 0.2306728364630075 1108 9 -0.2253478353690852 1108 10 -0.41217226801555285 1108 11 -0.0357590427659531 1108 12 -0.4220340031025387 1108 13 0.07821563771178575 1108 14 0.2689308616554271 1108 15 -0.174162138897465 1108 16 -0.09558503246919514 1108 17 0.28748213230258557 1108 18 0.3552465718076334 1108 19 -0.2745560101582569 1108 20 0.1409323802819813 1108 21 0.37485041317287937 1108 22 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2214 5 -0.20452374480775148 2214 6 -0.13976762193012604 2214 7 0.11769358137750174 2214 8 0.11138972204616948 2214 9 -0.14417729150665792 2214 10 -0.2058105019133194 2214 11 -0.21254070653131407 2214 12 -0.2625414673637607 2214 13 -0.21082007468996977 2214 14 -0.31445522264061054 2214 15 -0.42161690494694426 2214 16 -0.21637154932312797 2214 17 -0.4216358716009907 2214 18 -0.37298046833585374 2214 19 -0.2010607313694166 2214 20 -0.11334044769713358 2214 21 -0.3109252897336958 2214 22 -0.2562426632718555 2214 23 -0.4646161521230425 2214 24 -0.27687837370036306 2214 25 -0.521264777666893 2215 1 0.6966071377747909 2215 2 0.2626280315084806 2215 3 0.3664398742677797 2215 4 0.7335119158205288 2215 5 0.6600255035443112 2215 6 0.5027609501759116 2215 7 0.8183978067296138 2215 8 0.5568181850802674 2215 9 0.6703885547493229 2215 10 0.7267071629987072 2215 11 0.4599470821536542 2215 12 0.3941427456584776 2215 13 0.30626391070387626 2215 14 0.4829436487019194 2215 15 0.29517543156369896 2215 16 0.45282036986878893 2215 17 0.3939021375989924 2215 18 0.5795495264476642 2215 19 0.5327681679524403 2215 20 0.4052610603409974 2215 21 0.6038444736276176 2215 22 0.637507040337006 2215 23 0.5905126001888092 2215 24 0.54629467130367 2215 25 0.9897728284411329 2216 1 0.45461734739790316 2216 2 0.33105422896693426 2216 3 0.6096244077748949 2216 4 0.4688220059693293 2216 5 0.33701040633567203 2216 6 0.3810057639045965 2216 7 0.5244909966937712 2216 8 0.15951178777387298 2216 9 0.3739836454411929 2216 10 0.5767125936493045 2216 11 0.33373876239694766 2216 12 0.4617000390222188 2216 13 0.4591910313729315 2216 14 0.5815642634515683 2216 15 0.3747887692415546 2216 16 0.04617325354425361 2216 17 0.07186455749689458 2216 18 -0.04530113066105912 2216 19 0.40254116675769325 2216 20 0.5072486784576498 2216 21 0.3332894611371202 2216 22 0.2835372347542986 2216 23 0.013777507324682858 2216 24 0.31440589102778915 2216 25 0.4561571012770216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58.0 3 19.0 4 6.0 5 13.0 6 3.0 7 18.0 8 19.0 9 48.0 10 105.0 11 183.0 12 292.0 13 491.0 14 848.0 15 1310.0 16 1781.0 17 2029.0 18 1970.0 19 1786.0 20 1682.0 21 1582.0 22 1451.0 23 1408.0 24 1393.0 25 1324.0 26 1315.0 27 1267.0 28 1333.0 29 1468.0 30 1729.0 31 2146.0 32 2930.0 33 4008.0 34 5650.0 35 8249.0 36 14211.0 37 29855.0 38 77400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.383351933762995 22.414004662767393 10.427537205728543 22.77510619774107 2 10.95922407198706 29.727362440366008 31.01376326254139 28.299650225105545 3 21.235068134845108 36.9399455855393 24.013591938252432 17.811394341363165 4 13.11779855518504 28.66795535907214 24.297302505942195 33.91694357980062 5 9.328669777001815 46.09329837612477 31.551994954950747 13.026036891922665 6 30.071934884915862 33.57058610582369 17.60069132227062 18.756787686989828 7 19.29362581669771 40.137444604686195 20.981029841944544 19.587899736671552 8 27.013833334306554 38.13889391718685 19.82622202238793 15.021050726118668 9 26.78242997857172 23.59446251218843 16.975634820078355 32.647472689161496 10 17.2772178949704 38.12607289245968 26.901428187731362 17.695281024838554 11 23.273113708820404 34.64399574920298 15.073980217444616 27.008910324532003 12 24.106459881931833 39.12891151894521 23.57509474065294 13.189533858470018 13 28.956234854456802 27.01468570260722 18.81696884762489 25.21211059531109 14 20.257835489747304 36.472978653837174 21.31813723200523 21.951048624410294 15 35.26317325937819 25.67333236023938 18.912275580207268 20.151218800175158 16 14.641622682049604 32.959853332710544 32.954014666728945 19.444509318510907 17 25.905834126516048 35.80592230118364 23.11227379694133 15.175969775358977 18 26.520411321261527 27.754842250938673 26.88887201976024 18.83587440803957 19 21.84250322595713 37.899303426813105 27.684842964494035 12.573350382735729 20 23.738335221499398 30.89370598334521 24.382452902909332 20.985505892246064 21 17.603844873599197 34.42049509171285 28.95626631472603 19.019393719961926 22 21.553581752246807 33.542392973727395 16.523303142318227 28.380722131707575 23 15.287900023359027 34.379817799579534 25.38600794206961 24.946274234991826 24 20.608934860440257 42.47825858413592 19.482294398336613 17.430512157087204 25 21.070521243977222 28.577018542852972 17.681997371879106 32.6704628412907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7401.0 1 7401.0 2 5124.5 3 2848.0 4 2848.0 5 2848.0 6 2728.5 7 2609.0 8 2609.0 9 2609.0 10 2381.0 11 2153.0 12 2153.0 13 2153.0 14 2000.5 15 1848.0 16 1848.0 17 1848.0 18 2019.0 19 2190.0 20 2190.0 21 2190.0 22 2493.5 23 2797.0 24 2797.0 25 2797.0 26 3250.0 27 3703.0 28 3703.0 29 3703.0 30 4160.0 31 4617.0 32 4617.0 33 4617.0 34 4615.5 35 4614.0 36 4614.0 37 4614.0 38 4759.5 39 4905.0 40 4905.0 41 4905.0 42 7590.5 43 10276.0 44 10276.0 45 10276.0 46 35285.0 47 60294.0 48 60294.0 49 60294.0 50 49466.5 51 38639.0 52 38639.0 53 38639.0 54 25651.5 55 12664.0 56 12664.0 57 12664.0 58 8322.5 59 3981.0 60 3981.0 61 3981.0 62 3349.5 63 2718.0 64 2718.0 65 2718.0 66 2192.5 67 1667.0 68 1667.0 69 1667.0 70 1253.0 71 839.0 72 839.0 73 839.0 74 622.5 75 406.0 76 406.0 77 406.0 78 275.0 79 144.0 80 144.0 81 144.0 82 98.0 83 52.0 84 52.0 85 52.0 86 30.5 87 9.0 88 9.0 89 9.0 90 7.5 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13828918193488157 2 0.07410432956004201 3 0.05951686311121484 4 0.08577430271910375 5 0.07177033492822966 6 0.06651884700665188 7 0.06476835103279262 8 0.07410432956004201 9 0.06476835103279262 10 0.06651884700665188 11 0.06885284163846424 12 0.07177033492822966 13 0.07293733224413584 14 0.06301785505893337 15 0.06126735908507411 16 0.06301785505893337 17 0.07527132687594817 18 0.07410432956004201 19 0.0659353483486988 20 0.08052281479752596 21 0.08110631345547904 22 0.07993931613957288 23 0.08168981211343214 24 0.09511028124635314 25 0.09044229198272845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 171380.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.845956354300387 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64160071393344 20.036176916793092 2 11.148164674385288 5.539736258606606 3 3.442850097461309 2.5662270976776753 4 1.5006693126042132 1.4914225697280896 5 0.7796904722763673 0.9686077722021239 6 0.5002231042014044 0.7457112848640448 7 0.2324980625161457 0.40436456996148906 8 0.2113618750146779 0.42011903372622245 9 0.122120194452925 0.2730773719220446 >10 1.0685517014630939 4.915392694596803 >50 0.122120194452925 2.0988446726572527 >100 0.1432563819543928 7.828217995098612 >500 0.02113618750146779 3.989380324425254 >1k 0.05636316667058077 27.975843155560742 >5k 0.0046969305558817315 7.213793908274011 >10k+ 0.0046969305558817315 13.53308437390594 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 11859 6.919710584665656 No Hit CCCATGTACTCTGCGTTGATACCAC 11334 6.613373789240285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 6997 4.082740109697747 No Hit GAGTACATGGGAAGCAGTGGTATCA 5366 3.131053798576263 No Hit CATGTACTCTGCGTTGATACCACTG 4436 2.588400046679893 No Hit GCGTTGATACCACTGCTTCCCATGT 3594 2.0970941766833935 No Hit TATCAACGCAGAGTACATGGGAAGC 3380 1.972225463881433 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3293 1.9214610806395145 No Hit GTATCAACGCAGAGTACATGGGAAG 3245 1.8934531450577663 No Hit GGTATCAACGCAGAGTACATGGGAA 2997 1.7487454778854008 No Hit ACTCTGCGTTGATACCACTGCTTCC 2889 1.6857276228264675 No Hit GCTTCCCATGTACTCTGCGTTGATA 2101 1.2259306803594352 No Hit GTACTCTGCGTTGATACCACTGCTT 1911 1.1150659353483487 No Hit GTGGTATCAACGCAGAGTACATGGG 1744 1.0176216594701832 No Hit ATACCACTGCTTCCCATGTACTCTG 1682 0.9814447426770918 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1577 0.9201773835920177 No Hit GATACCACTGCTTCCCATGTACTCT 1489 0.8688295016921461 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1439 0.8396545687944919 No Hit CAGTGGTATCAACGCAGAGTACATG 1401 0.8174816197922745 No Hit TACCACTGCTTCCCATGTACTCTGC 1327 0.774302719103746 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1317 0.7684677325242152 No Hit CATGGGAAGCAGTGGTATCAACGCA 1315 0.767300735208309 No Hit GCAGTGGTATCAACGCAGAGTACAT 1315 0.767300735208309 No Hit GTTGATACCACTGCTTCCCATGTAC 1156 0.6745244485937683 No Hit CCATGTACTCTGCGTTGATACCACT 1110 0.6476835103279263 No Hit CCACTGCTTCCCATGTACTCTGCGT 1102 0.6430155210643016 No Hit CTTCCCATGTACTCTGCGTTGATAC 1102 0.6430155210643016 No Hit ACATGGGAAGCAGTGGTATCAACGC 1023 0.5969191270860077 No Hit AAGCAGTGGTATCAACGCAGAGTAC 912 0.532150776053215 No Hit ATGGGAAGCAGTGGTATCAACGCAG 852 0.4971408565760299 No Hit TGATACCACTGCTTCCCATGTACTC 783 0.45687944917726686 No Hit GTACATGGTAAGCAGTGGTATCAAC 775 0.4522114599136422 No Hit TTCCCATGTACTCTGCGTTGATACC 763 0.4452094760182051 No Hit GTATCAACGCAGAGTACTTTTTTTT 742 0.4329560042011903 No Hit ATCAACGCAGAGTACATGGGAAGCA 713 0.4160345431205508 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 705 0.4113665538569261 No Hit ACCATGTACTCTGCGTTGATACCAC 592 0.3454312055082273 No Hit GGTATCAACGCAGAGTACTTTTTTT 497 0.2899988330026841 No Hit GGAAGCAGTGGTATCAACGCAGAGT 490 0.28591434239701247 No Hit TATCAACGCAGAGTACTTTTTTTTT 429 0.2503209242618742 No Hit AAAAAGTACTCTGCGTTGATACCAC 428 0.24973742560392112 No Hit CTGCGTTGATACCACTGCTTCCCAT 389 0.22698097794375074 No Hit CCCCATGTACTCTGCGTTGATACCA 386 0.22523048196989148 No Hit TCCATGTACTCTGCGTTGATACCAC 363 0.21181001283697046 No Hit GGTAAGCAGTGGTATCAACGCAGAG 361 0.21064301552106432 No Hit TGGGAAGCAGTGGTATCAACGCAGA 358 0.20889251954720506 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 351 0.20480802894153344 No Hit CTCTGCGTTGATACCACTGCTTCCC 345 0.20130703699381491 No Hit TCAACGCAGAGTACATGGGAAGCAG 339 0.1978060450460964 No Hit TCCCATGTACTCTGCGTTGATACCA 293 0.17096510678025442 No Hit AAAGTACTCTGCGTTGATACCACTG 289 0.16863111214844206 No Hit AGTGGTATCAACGCAGAGTACATGG 275 0.16046213093709885 No Hit GAGTACATGGTAAGCAGTGGTATCA 264 0.1540436456996149 No Hit GTACATGGAAAGCAGTGGTATCAAC 261 0.15229314972575564 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 260 0.15170965106780254 No Hit GTACATGGGTAAGCAGTGGTATCAA 259 0.15112615240984945 No Hit ATGTACTCTGCGTTGATACCACTGC 255 0.14879215777803712 No Hit ACTGCTTCCCATGTACTCTGCGTTG 250 0.1458746644882717 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 249 0.1452911658303186 No Hit TTGATACCACTGCTTCCCATGTACT 242 0.14120667522464697 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 232 0.13537168864511612 No Hit AACGCAGAGTACATGGGAAGCAGTG 228 0.13303769401330376 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 214 0.12486871280196056 No Hit GCTTACCATGTACTCTGCGTTGATA 209 0.12195121951219512 No Hit GAGTACATGGAAAGCAGTGGTATCA 206 0.12020072353833586 No Hit ACTCTGCGTTGATACCACTGCTTAC 201 0.11728323024857042 No Hit CGTTGATACCACTGCTTCCCATGTA 199 0.11611623293266427 No Hit CAACGCAGAGTACATGGGAAGCAGT 191 0.11144824366903956 No Hit GCGTTGATACCACTGCTTACCATGT 178 0.10386276111564945 No Hit ACGCAGAGTACATGGTAAGCAGTGG 175 0.10211226514179018 No Hit CGCAGAGTACATGGGAAGCAGTGGT 172 0.10036176916793091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 120 0.0 15.846479 1 TACAAAA 65 3.3316046E-6 14.614711 2 GCTGGGA 40 0.0052553783 14.249344 9 GAGAAAA 60 0.0058035688 11.092536 1 CATGGGT 105 4.4418767E-4 9.047202 4 GAAAAAA 535 0.0 8.708159 1 AGTACTC 100 0.0028785795 8.549605 3 GGAAAAA 135 6.621691E-5 8.451456 1 GAAGCAG 3075 0.0 7.877685 9 GGAAGCA 3135 0.0 7.7269163 8 AGTGGTA 3330 0.0 7.67382 14 CAGTGGT 3345 0.0 7.6394086 13 AGCAGTG 3460 0.0 7.4404087 11 AAGCAGT 3465 0.0 7.4296722 10 AGTACTT 295 8.221832E-10 7.4064384 13 TGGTATC 3450 0.0 7.3518353 16 GTGGTAT 3455 0.0 7.3411956 15 CATGGGG 170 1.2054774E-4 7.2643704 4 GGGAAGC 3340 0.0 7.25266 7 GCAGTGG 3555 0.0 7.2415786 12 >>END_MODULE