FastQCFastQC Report
Wed 25 May 2016
SRR1294879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171380
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC121397.0830902088925205No Hit
GTACATGGGAAGCAGTGGTATCAAC115536.74115999533201No Hit
GAGTACATGGGAAGCAGTGGTATCA55163.218578597269226No Hit
CATGTACTCTGCGTTGATACCACTG46132.691679309137589No Hit
GCGTTGATACCACTGCTTCCCATGT38282.233632862644416No Hit
ACGCAGAGTACATGGGAAGCAGTGG36992.1583615357684676No Hit
TATCAACGCAGAGTACATGGGAAGC35042.0445792974676156No Hit
GTATCAACGCAGAGTACATGGGAAG32711.9086241101645467No Hit
GTATCAACGCAGAGTACTTTTTTTT30431.775586416151243No Hit
ACTCTGCGTTGATACCACTGCTTCC30281.7668339362819467No Hit
GGTATCAACGCAGAGTACATGGGAA29651.730073520830902No Hit
GGTATCAACGCAGAGTACTTTTTTT22651.3216244602637415No Hit
TATCAACGCAGAGTACTTTTTTTTT22191.2947835219978994No Hit
GCTTCCCATGTACTCTGCGTTGATA20211.1792507877231881No Hit
GTACTCTGCGTTGATACCACTGCTT18431.0753880266075388No Hit
GTGGTATCAACGCAGAGTACATGGG17511.0217061500758549No Hit
ATACCACTGCTTCCCATGTACTCTG16980.9907807212043411No Hit
GGGAAGCAGTGGTATCAACGCAGAG16580.9674407748862178No Hit
GATACCACTGCTTCCCATGTACTCT15020.8764149842455363No Hit
CAGTGGTATCAACGCAGAGTACATG14970.8734974909557708No Hit
CTGCTTCCCATGTACTCTGCGTTGA14730.8594935231648967No Hit
CATGGGAAGCAGTGGTATCAACGCA14360.8379040728206326No Hit
GCAGAGTACATGGGAAGCAGTGGTA13950.8139806278445559No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13800.8052281479752597No Hit
TACCACTGCTTCCCATGTACTCTGC13700.7993931613957287No Hit
GCAGTGGTATCAACGCAGAGTACAT13100.7643832419185436No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12950.7556307620492473No Hit
CCATGTACTCTGCGTTGATACCACT12420.7247053331777337No Hit
CCACTGCTTCCCATGTACTCTGCGT12000.7001983895437041No Hit
ACATGGGAAGCAGTGGTATCAACGC11790.6879449177266892No Hit
GTTGATACCACTGCTTCCCATGTAC11380.6640214727506126No Hit
CTTCCCATGTACTCTGCGTTGATAC10720.6255105613257089No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10110.5899171431905706No Hit
ATGGGAAGCAGTGGTATCAACGCAG9950.5805811646633212No Hit
AAGCAGTGGTATCAACGCAGAGTAC9810.5724121834519781No Hit
ATCAACGCAGAGTACATGGGAAGCA8400.49013887268059286No Hit
TTCCCATGTACTCTGCGTTGATACC7990.4662154277045163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7960.46446493173065706No Hit
TGATACCACTGCTTCCCATGTACTC7590.4428754813863929No Hit
GTACATGGTAAGCAGTGGTATCAAC7510.43820749212276816No Hit
ACCATGTACTCTGCGTTGATACCAC6090.3553506826934298No Hit
GGAAGCAGTGGTATCAACGCAGAGT5650.329676741743494No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5630.3285097444275878No Hit
TCAACGCAGAGTACATGGGAAGCAG4280.24973742560392112No Hit
AAAAAGTACTCTGCGTTGATACCAC4280.24973742560392112No Hit
TCCATGTACTCTGCGTTGATACCAC4210.2456529349982495No Hit
TGGGAAGCAGTGGTATCAACGCAGA3870.22581398062784455No Hit
CCCCATGTACTCTGCGTTGATACCA3850.2246469833119384No Hit
GTGGTATCAACGCAGAGTACTTTTT3810.22231298868012603No Hit
AAAGTACTCTGCGTTGATACCACTG3650.21297701015287662No Hit
CTCTGCGTTGATACCACTGCTTCCC3590.20947601820515815No Hit
ATCAACGCAGAGTACTTTTTTTTTT3590.20947601820515815No Hit
GGTAAGCAGTGGTATCAACGCAGAG3520.20539152759948653No Hit
TCCCATGTACTCTGCGTTGATACCA3510.20480802894153344No Hit
CTGCGTTGATACCACTGCTTCCCAT3250.18963706383475318No Hit
GTACATGGGTAAGCAGTGGTATCAA2930.17096510678025442No Hit
AACGCAGAGTACATGGGAAGCAGTG2820.16454662154277044No Hit
AGTGGTATCAACGCAGAGTACATGG2810.16396312288481735No Hit
GTACATGGAAAGCAGTGGTATCAAC2710.1581281363052865No Hit
GAGTACATGGTAAGCAGTGGTATCA2600.15170965106780254No Hit
ACTGCTTCCCATGTACTCTGCGTTG2570.14995915509394328No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2500.1458746644882717No Hit
ATGTACTCTGCGTTGATACCACTGC2450.14295717119850623No Hit
GCTTACCATGTACTCTGCGTTGATA2420.14120667522464697No Hit
TTGATACCACTGCTTCCCATGTACT2330.1359551873030692No Hit
CAACGCAGAGTACATGGGAAGCAGT2220.12953670206558524No Hit
CTGCTTACCATGTACTCTGCGTTGA2130.12428521414400748No Hit
CGTTGATACCACTGCTTCCCATGTA2110.1231182168281013No Hit
GCGTTGATACCACTGCTTACCATGT2020.11786672890652351No Hit
GAGTACATGGAAAGCAGTGGTATCA1940.11319873964289882No Hit
CAACGCAGAGTACTTTTTTTTTTTT1940.11319873964289882No Hit
CGCAGAGTACATGGGAAGCAGTGGT1860.10853075037927412No Hit
GTATCAACGCAGAGTACATGGTAAG1860.10853075037927412No Hit
ATGGTAAGCAGTGGTATCAACGCAG1840.10736375306336794No Hit
ACGCAGAGTACATGGTAAGCAGTGG1810.10561325708950868No Hit
ACTCTGCGTTGATACCACTGCTTAC1730.100945267825884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAA250.00600161519.0049631
GAACAAA702.4501787E-816.2899681
CAAAGAA551.9446909E-413.8177584
AAAAAGT500.00148714113.3034731
AAAAGTA550.00304336912.0940672
GGGTAAG800.0044820729.4997087
AGTACTC1107.0633047E-48.6360995
CCCCATG1356.6659944E-58.446651
ATGGGTA1150.00109809958.2606165
CCATGTA31850.08.1151492
CCCATGT32250.07.4546591
TACTCTG36300.07.2490897
TGATACC36800.07.176410717
TGTACTC36150.07.1477755
ATGTACT36200.07.13790234
TGCGTTG36850.07.115113712
CTGCGTT37050.07.07670611
TTGATAC37350.07.07073316
GATACCA37900.06.96812418
CATGTAC37150.06.9553723
Produced by FastQC (version 0.11.5)