##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 171380 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.487898237834052 33.0 31.0 34.0 29.0 34.0 2 31.691119150425955 33.0 31.0 34.0 28.0 34.0 3 31.82541136655386 33.0 31.0 34.0 29.0 34.0 4 35.20509977827051 37.0 35.0 37.0 33.0 37.0 5 35.546551522931495 37.0 35.0 37.0 33.0 37.0 6 35.273987629828454 37.0 35.0 37.0 33.0 37.0 7 35.23769984829035 37.0 35.0 37.0 33.0 37.0 8 35.30249153926946 37.0 35.0 37.0 33.0 37.0 9 36.87053331777337 39.0 37.0 39.0 33.0 39.0 10 36.918076788423384 39.0 37.0 39.0 33.0 39.0 11 36.96364803360952 39.0 37.0 39.0 33.0 39.0 12 36.98814914225697 39.0 37.0 39.0 33.0 39.0 13 36.80947018321858 39.0 37.0 39.0 33.0 39.0 14 38.07645582915159 40.0 38.0 41.0 33.0 41.0 15 37.98578597269226 40.0 38.0 41.0 33.0 41.0 16 37.82841638464232 40.0 38.0 41.0 32.0 41.0 17 38.03990547321741 40.0 38.0 41.0 33.0 41.0 18 38.050565993698214 40.0 38.0 41.0 33.0 41.0 19 37.97607655502392 40.0 38.0 41.0 32.0 41.0 20 38.07982261640798 40.0 38.0 41.0 33.0 41.0 21 37.97062084257206 40.0 38.0 41.0 32.0 41.0 22 38.11989146924962 40.0 38.0 41.0 33.0 41.0 23 38.0442700431789 40.0 38.0 41.0 32.0 41.0 24 38.05820399113082 40.0 38.0 41.0 32.0 41.0 25 36.9845489555374 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07185612141919506 1101 2 0.2457230070008336 1101 3 -0.3901458300587741 1101 4 -0.5256152736541111 1101 5 -0.03519529893366524 1101 6 -0.16435508553640688 1101 7 -0.2757119281844922 1101 8 -0.5743538360849101 1101 9 -0.39706530551780617 1101 10 -0.4493571916979775 1101 11 -0.34960155411393146 1101 12 -0.6907402639249511 1101 13 -0.7619935473569939 1101 14 -0.41452020903052045 1101 15 -0.5836498157273766 1101 16 -0.19662786146946587 1101 17 -0.16800370309766066 1101 18 -0.6837215440512026 1101 19 -0.3569690288189946 1101 20 -0.10506925534582479 1101 21 -0.18481340700567017 1101 22 0.032898806999000385 1101 23 -0.17050009520676923 1101 24 -0.24009513142001992 1101 25 -0.2506171746704027 1102 1 -0.09113049783642779 1102 2 -0.18035409050483509 1102 3 -0.1118011588569594 1102 4 -0.2698329607289409 1102 5 0.018546197664974784 1102 6 -0.25097640072915084 1102 7 -0.16822893498721214 1102 8 -0.03138331454295695 1102 9 -0.12790430778537853 1102 10 0.2963344182793435 1102 11 0.08259799237133336 1102 12 0.2531372870954556 1102 13 0.10478649799447481 1102 14 0.18253194516449156 1102 15 0.27054519561049517 1102 16 0.27105921336046634 1102 17 0.11737044656220519 1102 18 0.4963238074227192 1102 19 0.768767932632251 1102 20 0.22860421404192977 1102 21 0.12663784015985868 1102 22 0.1068217094933317 1102 23 0.2165747347252065 1102 24 -0.18760080035426085 1102 25 0.35301094324343296 1103 1 -0.17467587527493578 1103 2 -0.22554977692189482 1103 3 -0.141820545746576 1103 4 -0.18604524717023452 1103 5 -0.23517972018308342 1103 6 -0.21783693629197387 1103 7 -0.04209298442378184 1103 8 0.007481340733910713 1103 9 -0.16692042313736977 1103 10 -0.11536435792324795 1103 11 0.3033661978723572 1103 12 -0.14953446862973863 1103 13 0.09530509729169978 1103 14 0.08153836707167272 1103 15 0.047414212937525235 1103 16 0.07031402979085755 1103 17 -0.029757870413440912 1103 18 -0.009613427522154439 1103 19 0.2213609291183758 1103 20 -0.11572512074541663 1103 21 0.11521775049549632 1103 22 -0.22790505653114934 1103 23 -0.0853154870123447 1103 24 -0.667638362452891 1103 25 -0.01727864842020921 1104 1 -0.4800193867253171 1104 2 -0.23472900851611556 1104 3 -0.48012727718307957 1104 4 -0.4101320555734915 1104 5 -0.24394888301192452 1104 6 -0.9149538374338633 1104 7 -0.6684257079112541 1104 8 -0.7056913297600644 1104 9 -0.9410356209166935 1104 10 -0.5058759975674363 1104 11 -0.6936056020140811 1104 12 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12 -0.29200160179343015 2212 13 -0.07626508930710685 2212 14 -0.4072498008672554 2212 15 -0.19076432819903033 2212 16 -0.3891590406077867 2212 17 -0.2040581248663642 2212 18 -0.2745095259106165 2212 19 -0.2232955594312429 2212 20 -0.43657435738663963 2212 21 0.003125935397953583 2212 22 -0.3123251159034979 2212 23 -0.1137824081319394 2212 24 -0.10277937178283025 2212 25 -0.10829858056609254 2213 1 0.037463130757199536 2213 2 -0.10154401169475591 2213 3 0.12476782771202721 2213 4 0.3883421974462138 2213 5 0.07582225494103056 2213 6 0.18580903605628407 2213 7 0.18408541732713957 2213 8 0.2393182061585648 2213 9 0.44357976369869334 2213 10 0.30213417407910015 2213 11 0.2590326688060074 2213 12 0.15698344094160888 2213 13 0.18434582755380546 2213 14 0.26517152780407116 2213 15 3.7925103221425616E-5 2213 16 0.36538155768280944 2213 17 0.4486225278142868 2213 18 -0.02776321666430448 2213 19 0.03719395105827061 2213 20 0.20787494331266032 2213 21 0.3106482741702905 2213 22 0.02928813195975266 2213 23 -0.08245956430909018 2213 24 -0.0505989133523741 2213 25 -0.14438055414806428 2214 1 -0.02579403461264107 2214 2 0.004533009875224536 2214 3 0.18408527083228776 2214 4 0.0542783739457704 2214 5 0.05191306553888353 2214 6 0.3792493985107299 2214 7 0.02174423738032516 2214 8 0.2925044565279151 2214 9 0.24142276576704802 2214 10 0.3281363409263349 2214 11 0.41531652579408984 2214 12 0.2496161603348952 2214 13 0.23383858831417115 2214 14 0.4188945653299285 2214 15 0.2674711960576204 2214 16 0.1129438545408803 2214 17 0.08232685165945242 2214 18 -0.11204864954128624 2214 19 0.259098806765941 2214 20 0.3966122623316721 2214 21 0.17883994165773487 2214 22 0.06585367908867568 2214 23 0.3366283899901319 2214 24 0.2945700022390767 2214 25 0.02677575433218493 2215 1 0.46274155603562406 2215 2 0.36245495230420843 2215 3 0.1713878743320727 2215 4 0.29678560588962455 2215 5 0.4035870827058545 2215 6 0.4411832914305407 2215 7 0.3291255623672882 2215 8 0.21549018233054085 2215 9 0.37546269558162493 2215 10 0.35225072419564896 2215 11 0.3192502290235666 2215 12 -0.003596719638551349 2215 13 0.18667682988481005 2215 14 0.04424430687685543 2215 15 0.3544797795450805 2215 16 0.2990774913787462 2215 17 0.23870281789457692 2215 18 0.5771318882307952 2215 19 0.37350578737010665 2215 20 0.35162008705780323 2215 21 0.29342475694677717 2215 22 0.10970771237933263 2215 23 0.2134482315987043 2215 24 0.38322652047305894 2215 25 0.07162566185814967 2216 1 0.0683677100488751 2216 2 -0.0013986373558338983 2216 3 -0.3968754036086253 2216 4 0.07271696246614567 2216 5 -0.3247717746852601 2216 6 -0.11784788050385941 2216 7 -0.4498469585407179 2216 8 -0.24861321725673236 2216 9 -0.6279043077853785 2216 10 -0.7073778193038649 2216 11 -0.42073022063430443 2216 12 -0.6563609207898509 2216 13 -0.3525867738949202 2216 14 -0.7982668259568584 2216 15 -0.8930492744355902 2216 16 -0.5949162090665681 2216 17 -0.8330903829308838 2216 18 -0.7115614972367794 2216 19 -0.9876324769939657 2216 20 -0.33655195595294884 2216 21 -0.6356509463224782 2216 22 -0.3780733237888043 2216 23 -0.7381103651211873 2216 24 -0.5592085832523637 2216 25 -1.1564616630033697 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 11.0 9 29.0 10 65.0 11 78.0 12 104.0 13 157.0 14 282.0 15 434.0 16 624.0 17 685.0 18 721.0 19 787.0 20 746.0 21 735.0 22 723.0 23 758.0 24 681.0 25 780.0 26 777.0 27 811.0 28 960.0 29 1157.0 30 1458.0 31 1946.0 32 2672.0 33 3689.0 34 5617.0 35 8524.0 36 14444.0 37 29790.0 38 91134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.15896475038087 18.165899101686307 12.110599401124206 23.564536746808624 2 11.42210187216957 24.903123395116484 33.557238900042016 30.117535832671926 3 22.913226395607346 31.62092929622995 26.734042491116078 18.731801817046627 4 13.739642898821334 23.23083206908624 27.286731240518147 35.74279379157428 5 9.947485120784222 40.382191620959276 35.397946084724005 14.272377173532503 6 32.466183490307294 28.550004084824295 18.70441044314508 20.27940198172333 7 19.82378340529817 35.112615240984944 23.877932080756214 21.185669272960673 8 29.757264558291517 31.039211109814445 22.12335161629128 17.080172715602753 9 28.371455245652932 15.612090092192787 19.965573579180766 36.050881082973504 10 18.939782938499242 31.513012020072356 30.104446259773603 19.442758781654803 11 25.467965923678378 27.19978994048314 17.4763683043529 29.85587583148559 12 26.213677208542418 33.046446493173065 27.234799859960322 13.505076438324192 13 32.33749562376006 19.703582681759833 22.475784805694946 25.483136888785157 14 22.69926479169098 28.83592017738359 25.964523281596453 22.500291749328976 15 37.97584315556074 18.153810246236436 23.314855875831487 20.55549072237134 16 15.31567277395262 26.254522114599137 37.686427821216014 20.74337729023223 17 26.194421752829967 29.0360602170615 28.400046679892636 16.369471350215896 18 27.150192554557123 20.071770334928228 32.9577546971642 19.820282413350448 19 22.40284747345081 30.815731123818413 34.15684443925779 12.624576963472983 20 24.261874197689345 22.968257673007354 31.260940599836623 21.508927529466682 21 17.399929980161048 27.81829851791341 35.37518963706383 19.40658186486171 22 21.68747811880033 26.821682810129538 23.331193838254173 28.159645232815965 23 15.18730306920294 26.791340879915975 32.45477885400864 25.566577196872448 24 20.753880266075388 35.76146574862878 26.221846189753762 17.26280779554207 25 21.231182168281013 21.891702649083907 24.366320457462948 32.51079472517213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 986.0 1 986.0 2 848.0 3 710.0 4 710.0 5 710.0 6 1359.0 7 2008.0 8 2008.0 9 2008.0 10 1850.0 11 1692.0 12 1692.0 13 1692.0 14 1252.5 15 813.0 16 813.0 17 813.0 18 1253.5 19 1694.0 20 1694.0 21 1694.0 22 2506.5 23 3319.0 24 3319.0 25 3319.0 26 4487.0 27 5655.0 28 5655.0 29 5655.0 30 6226.0 31 6797.0 32 6797.0 33 6797.0 34 6613.5 35 6430.0 36 6430.0 37 6430.0 38 5923.0 39 5416.0 40 5416.0 41 5416.0 42 8014.5 43 10613.0 44 10613.0 45 10613.0 46 35928.5 47 61244.0 48 61244.0 49 61244.0 50 51092.0 51 40940.0 52 40940.0 53 40940.0 54 27143.0 55 13346.0 56 13346.0 57 13346.0 58 8709.0 59 4072.0 60 4072.0 61 4072.0 62 3418.0 63 2764.0 64 2764.0 65 2764.0 66 2151.0 67 1538.0 68 1538.0 69 1538.0 70 1179.5 71 821.0 72 821.0 73 821.0 74 591.0 75 361.0 76 361.0 77 361.0 78 244.5 79 128.0 80 128.0 81 128.0 82 77.5 83 27.0 84 27.0 85 27.0 86 16.5 87 6.0 88 6.0 89 6.0 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03442642081923212 2 0.01633796242268643 3 0.00175049597385926 4 0.0 5 0.0 6 0.008168981211343214 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 171380.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.504376239934647 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87450736361751 17.97525965690279 2 11.543248288736777 5.195472050414284 3 3.7725575606720594 2.5469716419652237 4 1.475316324414022 1.3280429455012253 5 0.7726612735946898 0.8694130003500993 6 0.46411532876996475 0.6266775586416151 7 0.31632441402198713 0.49830785389193605 8 0.19964737606305744 0.3594351732991014 9 0.1763119684712715 0.3571011786672891 >10 1.0449076954988592 4.467265725288832 >50 0.1114913918274217 1.7469949819115416 >100 0.13741962248496162 6.97922744777687 >500 0.02592823065753993 4.468432722604738 >1k 0.0777846919726198 35.5385692612907 >5k 0.0025928230657539927 3.218578597269226 >10k+ 0.005185646131507985 13.82425020422453 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 12139 7.0830902088925205 No Hit GTACATGGGAAGCAGTGGTATCAAC 11553 6.74115999533201 No Hit GAGTACATGGGAAGCAGTGGTATCA 5516 3.218578597269226 No Hit CATGTACTCTGCGTTGATACCACTG 4613 2.691679309137589 No Hit GCGTTGATACCACTGCTTCCCATGT 3828 2.233632862644416 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3699 2.1583615357684676 No Hit TATCAACGCAGAGTACATGGGAAGC 3504 2.0445792974676156 No Hit GTATCAACGCAGAGTACATGGGAAG 3271 1.9086241101645467 No Hit GTATCAACGCAGAGTACTTTTTTTT 3043 1.775586416151243 No Hit ACTCTGCGTTGATACCACTGCTTCC 3028 1.7668339362819467 No Hit GGTATCAACGCAGAGTACATGGGAA 2965 1.730073520830902 No Hit GGTATCAACGCAGAGTACTTTTTTT 2265 1.3216244602637415 No Hit TATCAACGCAGAGTACTTTTTTTTT 2219 1.2947835219978994 No Hit GCTTCCCATGTACTCTGCGTTGATA 2021 1.1792507877231881 No Hit GTACTCTGCGTTGATACCACTGCTT 1843 1.0753880266075388 No Hit GTGGTATCAACGCAGAGTACATGGG 1751 1.0217061500758549 No Hit ATACCACTGCTTCCCATGTACTCTG 1698 0.9907807212043411 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1658 0.9674407748862178 No Hit GATACCACTGCTTCCCATGTACTCT 1502 0.8764149842455363 No Hit CAGTGGTATCAACGCAGAGTACATG 1497 0.8734974909557708 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1473 0.8594935231648967 No Hit CATGGGAAGCAGTGGTATCAACGCA 1436 0.8379040728206326 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1395 0.8139806278445559 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1380 0.8052281479752597 No Hit TACCACTGCTTCCCATGTACTCTGC 1370 0.7993931613957287 No Hit GCAGTGGTATCAACGCAGAGTACAT 1310 0.7643832419185436 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1295 0.7556307620492473 No Hit CCATGTACTCTGCGTTGATACCACT 1242 0.7247053331777337 No Hit CCACTGCTTCCCATGTACTCTGCGT 1200 0.7001983895437041 No Hit ACATGGGAAGCAGTGGTATCAACGC 1179 0.6879449177266892 No Hit GTTGATACCACTGCTTCCCATGTAC 1138 0.6640214727506126 No Hit CTTCCCATGTACTCTGCGTTGATAC 1072 0.6255105613257089 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1011 0.5899171431905706 No Hit ATGGGAAGCAGTGGTATCAACGCAG 995 0.5805811646633212 No Hit AAGCAGTGGTATCAACGCAGAGTAC 981 0.5724121834519781 No Hit ATCAACGCAGAGTACATGGGAAGCA 840 0.49013887268059286 No Hit TTCCCATGTACTCTGCGTTGATACC 799 0.4662154277045163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 796 0.46446493173065706 No Hit TGATACCACTGCTTCCCATGTACTC 759 0.4428754813863929 No Hit GTACATGGTAAGCAGTGGTATCAAC 751 0.43820749212276816 No Hit ACCATGTACTCTGCGTTGATACCAC 609 0.3553506826934298 No Hit GGAAGCAGTGGTATCAACGCAGAGT 565 0.329676741743494 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 563 0.3285097444275878 No Hit TCAACGCAGAGTACATGGGAAGCAG 428 0.24973742560392112 No Hit AAAAAGTACTCTGCGTTGATACCAC 428 0.24973742560392112 No Hit TCCATGTACTCTGCGTTGATACCAC 421 0.2456529349982495 No Hit TGGGAAGCAGTGGTATCAACGCAGA 387 0.22581398062784455 No Hit CCCCATGTACTCTGCGTTGATACCA 385 0.2246469833119384 No Hit GTGGTATCAACGCAGAGTACTTTTT 381 0.22231298868012603 No Hit AAAGTACTCTGCGTTGATACCACTG 365 0.21297701015287662 No Hit CTCTGCGTTGATACCACTGCTTCCC 359 0.20947601820515815 No Hit ATCAACGCAGAGTACTTTTTTTTTT 359 0.20947601820515815 No Hit GGTAAGCAGTGGTATCAACGCAGAG 352 0.20539152759948653 No Hit TCCCATGTACTCTGCGTTGATACCA 351 0.20480802894153344 No Hit CTGCGTTGATACCACTGCTTCCCAT 325 0.18963706383475318 No Hit GTACATGGGTAAGCAGTGGTATCAA 293 0.17096510678025442 No Hit AACGCAGAGTACATGGGAAGCAGTG 282 0.16454662154277044 No Hit AGTGGTATCAACGCAGAGTACATGG 281 0.16396312288481735 No Hit GTACATGGAAAGCAGTGGTATCAAC 271 0.1581281363052865 No Hit GAGTACATGGTAAGCAGTGGTATCA 260 0.15170965106780254 No Hit ACTGCTTCCCATGTACTCTGCGTTG 257 0.14995915509394328 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 250 0.1458746644882717 No Hit ATGTACTCTGCGTTGATACCACTGC 245 0.14295717119850623 No Hit GCTTACCATGTACTCTGCGTTGATA 242 0.14120667522464697 No Hit TTGATACCACTGCTTCCCATGTACT 233 0.1359551873030692 No Hit CAACGCAGAGTACATGGGAAGCAGT 222 0.12953670206558524 No Hit CTGCTTACCATGTACTCTGCGTTGA 213 0.12428521414400748 No Hit CGTTGATACCACTGCTTCCCATGTA 211 0.1231182168281013 No Hit GCGTTGATACCACTGCTTACCATGT 202 0.11786672890652351 No Hit GAGTACATGGAAAGCAGTGGTATCA 194 0.11319873964289882 No Hit CAACGCAGAGTACTTTTTTTTTTTT 194 0.11319873964289882 No Hit CGCAGAGTACATGGGAAGCAGTGGT 186 0.10853075037927412 No Hit GTATCAACGCAGAGTACATGGTAAG 186 0.10853075037927412 No Hit ATGGTAAGCAGTGGTATCAACGCAG 184 0.10736375306336794 No Hit ACGCAGAGTACATGGTAAGCAGTGG 181 0.10561325708950868 No Hit ACTCTGCGTTGATACCACTGCTTAC 173 0.100945267825884 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.834986579530867E-4 0.0 13 0.0 0.0 0.0 5.834986579530867E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCAAA 25 0.006001615 19.004963 1 GAACAAA 70 2.4501787E-8 16.289968 1 CAAAGAA 55 1.9446909E-4 13.817758 4 AAAAAGT 50 0.001487141 13.303473 1 AAAAGTA 55 0.003043369 12.094067 2 GGGTAAG 80 0.004482072 9.499708 7 AGTACTC 110 7.0633047E-4 8.636099 5 CCCCATG 135 6.6659944E-5 8.44665 1 ATGGGTA 115 0.0010980995 8.260616 5 CCATGTA 3185 0.0 8.115149 2 CCCATGT 3225 0.0 7.454659 1 TACTCTG 3630 0.0 7.249089 7 TGATACC 3680 0.0 7.1764107 17 TGTACTC 3615 0.0 7.147775 5 ATGTACT 3620 0.0 7.1379023 4 TGCGTTG 3685 0.0 7.1151137 12 CTGCGTT 3705 0.0 7.076706 11 TTGATAC 3735 0.0 7.070733 16 GATACCA 3790 0.0 6.968124 18 CATGTAC 3715 0.0 6.955372 3 >>END_MODULE