FastQCFastQC Report
Wed 25 May 2016
SRR1294878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141412
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC92516.541877634147031No Hit
CCCATGTACTCTGCGTTGATACCAC89016.294373886233134No Hit
GAGTACATGGGAAGCAGTGGTATCA38102.6942550844341357No Hit
CATGTACTCTGCGTTGATACCACTG31002.192176052951659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA29442.0818600967386076No Hit
GCGTTGATACCACTGCTTCCCATGT27961.9772013690493029No Hit
GTATCAACGCAGAGTACATGGGAAG25051.771419681498034No Hit
TATCAACGCAGAGTACATGGGAAGC23891.689389867903714No Hit
ACGCAGAGTACATGGGAAGCAGTGG21651.53098746923882No Hit
ACTCTGCGTTGATACCACTGCTTCC19271.36268492065737No Hit
GGTATCAACGCAGAGTACATGGGAA18801.3294487030803608No Hit
GCTTCCCATGTACTCTGCGTTGATA13850.9794076881735638No Hit
GTACTCTGCGTTGATACCACTGCTT12920.913642406585014No Hit
ATACCACTGCTTCCCATGTACTCTG12250.8662631176986395No Hit
GGGAAGCAGTGGTATCAACGCAGAG11080.783526150538851No Hit
GTGGTATCAACGCAGAGTACATGGG10810.7644330042712075No Hit
CAGTGGTATCAACGCAGAGTACATG9580.6774531157186094No Hit
GCAGTGGTATCAACGCAGAGTACAT9490.6710887336293949No Hit
GCAGAGTACATGGGAAGCAGTGGTA9270.6555313551890929No Hit
TACCACTGCTTCCCATGTACTCTGC9260.6548242016236246No Hit
CTGCTTCCCATGTACTCTGCGTTGA9210.6512884337962832No Hit
GATACCACTGCTTCCCATGTACTCT8610.6088592198681866No Hit
CATGGGAAGCAGTGGTATCAACGCA8380.5925946878624162No Hit
GTTGATACCACTGCTTCCCATGTAC7990.5650156988091534No Hit
CCATGTACTCTGCGTTGATACCACT7890.5579441631544706No Hit
CCACTGCTTCCCATGTACTCTGCGT7330.5183435634882472No Hit
ACATGGGAAGCAGTGGTATCAACGC6630.4688428139054677No Hit
CTTCCCATGTACTCTGCGTTGATAC6400.45257828189969734No Hit
AAGCAGTGGTATCAACGCAGAGTAC6230.44055667128673665No Hit
ATGGGAAGCAGTGGTATCAACGCAG5940.4200492178881566No Hit
TGATACCACTGCTTCCCATGTACTC4780.3380194042938365No Hit
ATCAACGCAGAGTACATGGGAAGCA4770.3373122507283682No Hit
GTATCAACGCAGAGTACTTTTTTTT4760.3366050971628999No Hit
GTACATGGTAAGCAGTGGTATCAAC4560.32246202585353434No Hit
TTCCCATGTACTCTGCGTTGATACC4170.29488303680027156No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3710.2623539727887308No Hit
ACCATGTACTCTGCGTTGATACCAC3480.2460894407829604No Hit
GGAAGCAGTGGTATCAACGCAGAGT3420.24184651939015075No Hit
TATCAACGCAGAGTACTTTTTTTTT3420.24184651939015075No Hit
TCCATGTACTCTGCGTTGATACCAC3320.234774983735468No Hit
GGTATCAACGCAGAGTACTTTTTTT3190.22558198738438037No Hit
AAAAAGTACTCTGCGTTGATACCAC2960.20931745537861002No Hit
CCCCATGTACTCTGCGTTGATACCA2850.20153876615845895No Hit
AAAGTACTCTGCGTTGATACCACTG2420.17113116284332305No Hit
GGTAAGCAGTGGTATCAACGCAGAG2370.16759539501598167No Hit
TCCCATGTACTCTGCGTTGATACCA2270.1605238593612989No Hit
GTACATGGAAAGCAGTGGTATCAAC2260.15981670579583063No Hit
TCAACGCAGAGTACATGGGAAGCAG2250.15910955223036233No Hit
CTGCGTTGATACCACTGCTTCCCAT2210.15628093796848924No Hit
CTCTGCGTTGATACCACTGCTTCCC2090.14779509518286993No Hit
AAACAAAAAAAAAAAAAAAAAAAAA2070.14638078805193336No Hit
TGGGAAGCAGTGGTATCAACGCAGA2070.14638078805193336No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1870.13223771674256782No Hit
GAGTACATGGTAAGCAGTGGTATCA1790.12658048821882162No Hit
GTACATGGGTAAGCAGTGGTATCAA1770.12516618108788505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1640.11597318473679744No Hit
AGTGGTATCAACGCAGAGTACATGG1560.11031595621305124No Hit
ACTGCTTCCCATGTACTCTGCGTTG1520.10748734195117812No Hit
GCTTACCATGTACTCTGCGTTGATA1460.10324442055836847No Hit
ATGTACTCTGCGTTGATACCACTGC1420.10041580629649535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA456.666049E-414.7901791
GAACAAA604.0226374E-412.6772981
TACAAAA951.3393203E-510.997542
CCATGTA23200.08.5153372
GAAGCAG23050.08.2823139
TGCGTTG24450.08.23829312
CTGCGTT24700.08.19337711
GGAAGCA23300.08.1526838
AGTGGTA24800.08.12202714
TGATACC24800.08.12202717
TCTGCGT24950.08.10840810
CTCTGCG25050.08.0760389
CAGTGGT24950.08.07319713
TAAAAAA1304.1246339E-48.0366642
TGGTATC25200.08.03080916
CCCATGT22900.08.013271
TACTCTG25400.08.0021487
TGTACTC24850.07.9881545
GTGGTAT25100.07.98709815
TTGATAC25300.07.961512616
Produced by FastQC (version 0.11.5)