FastQCFastQC Report
Wed 25 May 2016
SRR1294878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141412
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC95426.7476593216983005No Hit
GTACATGGGAAGCAGTGGTATCAAC90156.374989392696517No Hit
GAGTACATGGGAAGCAGTGGTATCA39282.7776992051593923No Hit
CATGTACTCTGCGTTGATACCACTG32222.278448787938789No Hit
GCGTTGATACCACTGCTTCCCATGT29122.059231182643623No Hit
TATCAACGCAGAGTACATGGGAAGC25541.8060702062059797No Hit
GTATCAACGCAGAGTACATGGGAAG24981.7664696065397563No Hit
ACGCAGAGTACATGGGAAGCAGTGG24621.7410120781828982No Hit
ACTCTGCGTTGATACCACTGCTTCC20281.434107430769666No Hit
GTATCAACGCAGAGTACTTTTTTTT19331.3669278420501796No Hit
GGTATCAACGCAGAGTACATGGGAA18961.3407631601278533No Hit
GCTTCCCATGTACTCTGCGTTGATA14651.035979973411026No Hit
TATCAACGCAGAGTACTTTTTTTTT13710.9695075382570079No Hit
GGTATCAACGCAGAGTACTTTTTTT13130.9284926314598478No Hit
GTACTCTGCGTTGATACCACTGCTT13080.9249568636325064No Hit
ATACCACTGCTTCCCATGTACTCTG12880.9108137923231409No Hit
GGGAAGCAGTGGTATCAACGCAGAG11180.7905976861935338No Hit
GTGGTATCAACGCAGAGTACATGGG10690.7559471614855883No Hit
CAGTGGTATCAACGCAGAGTACATG10120.7156394082538964No Hit
GCAGAGTACATGGGAAGCAGTGGTA10110.7149322546884281No Hit
TACCACTGCTTCCCATGTACTCTGC9830.6951319548553164No Hit
CATGGGAAGCAGTGGTATCAACGCA9740.6887675727661019No Hit
CTGCTTCCCATGTACTCTGCGTTGA9530.673917347891268No Hit
GCAGTGGTATCAACGCAGAGTACAT9440.6675529658020536No Hit
GATACCACTGCTTCCCATGTACTCT9030.6385596696178543No Hit
CCATGTACTCTGCGTTGATACCACT8800.6222951376120839No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8640.6109806805645914No Hit
GTTGATACCACTGCTTCCCATGTAC7790.5508726274997879No Hit
ACATGGGAAGCAGTGGTATCAACGC7760.548751166803383No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7650.540972477583232No Hit
CCACTGCTTCCCATGTACTCTGCGT7320.5176364099227788No Hit
AAGCAGTGGTATCAACGCAGAGTAC7040.4978361100896671No Hit
ATGGGAAGCAGTGGTATCAACGCAG6880.48652165304217465No Hit
CTTCCCATGTACTCTGCGTTGATAC6540.4624784318162532No Hit
ATCAACGCAGAGTACATGGGAAGCA5760.4073204537097276No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5510.38964161457302066No Hit
TGATACCACTGCTTCCCATGTACTC4770.3373122507283682No Hit
GTACATGGTAAGCAGTGGTATCAAC4760.3366050971628999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4340.30690464741323226No Hit
TTCCCATGTACTCTGCGTTGATACC4320.3054903402822957No Hit
GGAAGCAGTGGTATCAACGCAGAGT4010.28356857975277916No Hit
ACCATGTACTCTGCGTTGATACCAC3780.2673040477470088No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3300.23336067660453141No Hit
CCCCATGTACTCTGCGTTGATACCA3130.22133906599157072No Hit
AAAAAGTACTCTGCGTTGATACCAC3050.21568183746782452No Hit
TCCATGTACTCTGCGTTGATACCAC2910.20578168755126863No Hit
TCAACGCAGAGTACATGGGAAGCAG2770.19588153763471275No Hit
GGTAAGCAGTGGTATCAACGCAGAG2600.18385992702175205No Hit
CTGCGTTGATACCACTGCTTCCCAT2580.18244561989081548No Hit
TGGGAAGCAGTGGTATCAACGCAGA2470.17466693067066444No Hit
ATCAACGCAGAGTACTTTTTTTTTT2400.1697168557123865No Hit
CTCTGCGTTGATACCACTGCTTCCC2370.16759539501598167No Hit
GTACATGGAAAGCAGTGGTATCAAC2300.16264532005770374No Hit
GTACATGGGTAAGCAGTGGTATCAA2250.15910955223036233No Hit
AAAGTACTCTGCGTTGATACCACTG2240.15840239866489406No Hit
TCCCATGTACTCTGCGTTGATACCA2150.15203801657567956No Hit
GAGTACATGGTAAGCAGTGGTATCA1840.13011625604616298No Hit
AACGCAGAGTACATGGGAAGCAGTG1660.117387491867734No Hit
GAGTACATGGAAAGCAGTGGTATCA1600.11314457047492434No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1590.11243741690945606No Hit
ATGTACTCTGCGTTGATACCACTGC1540.10890164908211467No Hit
ACTGCTTCCCATGTACTCTGCGTTG1530.1081944955166464No Hit
GTACATGGAAGCAGTGGTATCAACG1520.10748734195117812No Hit
AGTGGTATCAACGCAGAGTACATGG1520.10748734195117812No Hit
CAACGCAGAGTACATGGGAAGCAGT1520.10748734195117812No Hit
CGTTGATACCACTGCTTCCCATGTA1420.10041580629649535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA600.005816477411.0870481
CATGGTA1901.0113581E-88.9998334
GAAGCAG22350.08.755999
GGAAGCA23150.08.412378
CATGGGT1252.697052E-48.3598444
AGTGGTA25300.08.335813514
CAGTGGT25700.08.20607313
TGGTATC25800.08.17426616
GTGGTAT25800.08.17426615
AGCAGTG26300.08.12722611
ACATGGT2002.2168024E-78.074853
TGGTAAG2002.2168024E-78.074856
AAGCAGT26500.08.06588710
GGGAAGC24400.07.98140867
ATGGTAA2201.2184319E-77.77258255
GCAGTGG27750.07.702559512
CCATGTA24650.07.5921512
TGCGTTG26700.07.365031712
TGGGAAG26550.07.335086
TACATGG33700.07.2728622
Produced by FastQC (version 0.11.5)