##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141412 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6104644584618 33.0 31.0 34.0 30.0 34.0 2 31.80027154696914 33.0 31.0 34.0 30.0 34.0 3 31.947804995332785 33.0 31.0 34.0 30.0 34.0 4 35.35620739399768 37.0 35.0 37.0 33.0 37.0 5 35.66586994031624 37.0 35.0 37.0 33.0 37.0 6 35.40790739116907 37.0 35.0 37.0 33.0 37.0 7 35.369791813990325 37.0 35.0 37.0 33.0 37.0 8 35.417072101377535 37.0 35.0 37.0 33.0 37.0 9 37.04508811133426 39.0 37.0 39.0 34.0 39.0 10 37.08505643085452 39.0 37.0 39.0 34.0 39.0 11 37.10826521087319 39.0 37.0 39.0 34.0 39.0 12 37.12235878143298 39.0 37.0 39.0 34.0 39.0 13 36.95971346137527 39.0 37.0 39.0 33.0 39.0 14 38.25893134953186 40.0 38.0 41.0 33.0 41.0 15 38.16554464967612 40.0 38.0 41.0 33.0 41.0 16 38.03284021158035 40.0 38.0 41.0 33.0 41.0 17 38.214097813481175 40.0 38.0 41.0 33.0 41.0 18 38.22238565326846 40.0 38.0 41.0 33.0 41.0 19 38.146621220264194 40.0 38.0 41.0 33.0 41.0 20 38.257410969366106 40.0 38.0 41.0 33.0 41.0 21 38.170650298418806 40.0 38.0 41.0 33.0 41.0 22 38.28914094984867 40.0 38.0 41.0 33.0 41.0 23 38.23707323282324 40.0 38.0 41.0 33.0 41.0 24 38.23950584108845 40.0 38.0 41.0 33.0 41.0 25 37.15498684694368 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10566073654466734 1101 2 0.37299445923598995 1101 3 0.06859316353459022 1101 4 0.09444168846458467 1101 5 -0.06152149212763902 1101 6 0.37471767598244554 1101 7 0.08826386638911998 1101 8 0.16930065661290428 1101 9 0.08691945587134597 1101 10 0.27588305073170005 1101 11 0.39866913986702457 1101 12 0.6333355968555949 1101 13 0.5002948981940136 1101 14 0.6980117544525157 1101 15 0.8385299741628316 1101 16 0.48780123281586185 1101 17 0.4340392275054441 1101 18 0.7837095398693208 1101 19 0.6347082889611642 1101 20 0.6286758077664771 1101 21 0.5287565096158957 1101 22 0.5849578835856164 1101 23 0.6195047806962961 1101 24 0.2419198555638573 1101 25 0.6528181082202664 1102 1 0.14811356673334686 1102 2 0.3446925724435417 1102 3 0.2761403333459107 1102 4 0.16519640544571246 1102 5 0.28281813051386706 1102 6 0.03037805334093946 1102 7 0.3335468852570429 1102 8 0.19288556227328257 1102 9 0.5586175690788977 1102 10 0.6390905979015145 1102 11 0.4552729134519282 1102 12 0.3408827666669154 1102 13 0.4342571623449558 1102 14 0.7546155280374194 1102 15 0.7489073326533955 1102 16 0.7094993460234065 1102 17 0.8255486614677068 1102 18 0.6563510493032823 1102 19 0.5545196097158822 1102 20 0.5862229775777976 1102 21 0.17498292471023547 1102 22 0.3396748647176935 1102 23 0.6195047806962961 1102 24 0.37871230839405 1102 25 0.5207426365221579 1103 1 -0.0279870621974716 1103 2 0.1400125872419089 1103 3 -0.06773684016500425 1103 4 0.10766773359961945 1103 5 0.06509707982944235 1103 6 0.024181234990955147 1103 7 0.04340630072134388 1103 8 -0.19048476699049388 1103 9 -0.49992849085451496 1103 10 -0.48891532144735805 1103 11 -0.09041706065092114 1103 12 -0.36897664879738556 1103 13 -0.23555415840976224 1103 14 -0.3760725962689051 1103 15 -0.25858434326224966 1103 16 -0.21771116082084063 1103 17 0.1671135893256448 1103 18 -0.28399301284988354 1103 19 -0.13249481869699054 1103 20 0.08307832348974387 1103 21 -0.06456572493830492 1103 22 0.008283817732859688 1103 23 0.18276782176177875 1103 24 -0.17493179075504628 1103 25 0.23272931798719299 1104 1 0.28341644657445997 1104 2 0.24644846302763312 1104 3 0.025869501893350133 1104 4 0.254187106899181 1104 5 0.22465707607674545 1104 6 0.2045452466243134 1104 7 0.3535432741710096 1104 8 0.3976567096958732 1104 9 0.4815660208257597 1104 10 0.37692575182405363 1104 11 0.14309452580184256 1104 12 0.3866758514371611 1104 13 0.4067903391978902 1104 14 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2213 19 0.3190015693319026 2213 20 0.4251835866476412 2213 21 0.17821033285323296 2213 22 0.3581290189712476 2213 23 0.672963900193146 2213 24 0.4352333279240028 2213 25 0.5361348907426091 2214 1 0.22710755206744793 2214 2 -0.02930841626866254 2214 3 -0.10346335784631222 2214 4 0.13436094363472506 2214 5 0.007194005936135284 2214 6 0.14854975969340956 2214 7 0.08311432366851079 2214 8 0.34492066155646484 2214 9 -0.1957286449772937 2214 10 -0.24523007442278555 2214 11 -0.49207816177258934 2214 12 -0.25693794685372495 2214 13 -0.15149302303858292 2214 14 -0.3053045510595567 2214 15 -0.07588462582069155 2214 16 0.01330176836830077 2214 17 -0.15892031768694181 2214 18 -0.13070506011420235 2214 19 -0.11111028234970632 2214 20 -0.2287848497271412 2214 21 -0.08552214243979961 2214 22 0.35946970693570535 2214 23 0.16241077064438514 2214 24 -0.008821678587906945 2214 25 0.16202549060471227 2215 1 0.256448199510519 2215 2 0.01961070748174265 2215 3 0.1648429686180144 2215 4 0.21068993421540227 2215 5 0.19420905519810816 2215 6 0.2989341113481103 2215 7 0.383991295049654 2215 8 0.027713256020362564 2215 9 -0.1023835224539269 2215 10 0.0024150950167438623 2215 11 0.3621027902646503 2215 12 0.10242182794868882 2215 13 -0.022744241054390102 2215 14 0.03860897884047887 2215 15 -0.003236749679366824 2215 16 -0.05055834928300129 2215 17 -0.17671237081047764 2215 18 -0.0249993327332092 2215 19 -0.023719903772345674 2215 20 0.25732074773217306 2215 21 0.4570272097562338 2215 22 0.2932457363846197 2215 23 0.5166278120388768 2215 24 0.4821740267785799 2215 25 0.20805746577523365 2216 1 0.01367960446919625 2216 2 0.10282529853332534 2216 3 0.03207722339145391 2216 4 0.12355684786601273 2216 5 0.11487733675980394 2216 6 -0.20384445804487683 2216 7 -0.12562682977223716 2216 8 -0.07858483460374543 2216 9 -0.08207859227439229 2216 10 -0.1958963897367454 2216 11 -0.03480516071853401 2216 12 -0.04908144933828851 2216 13 -0.12779553772010388 2216 14 -0.2619752331857441 2216 15 -0.2885032073596179 2216 16 -0.28456376393105387 2216 17 -0.3412535506337875 2216 18 -0.34763398648071586 2216 19 -0.1679917761006422 2216 20 -0.2516917914527852 2216 21 -0.259629957527892 2216 22 -0.21847412682427603 2216 23 -0.20059931819765353 2216 24 0.07422304359248955 2216 25 -0.4150901546294392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 23.0 11 26.0 12 48.0 13 88.0 14 137.0 15 248.0 16 399.0 17 480.0 18 526.0 19 540.0 20 532.0 21 629.0 22 546.0 23 577.0 24 593.0 25 549.0 26 574.0 27 681.0 28 756.0 29 960.0 30 1298.0 31 1519.0 32 2184.0 33 3169.0 34 4614.0 35 7116.0 36 11862.0 37 23981.0 38 76749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.44226551777806 17.618885917625253 11.315562347015295 24.62328621758139 2 11.942768634495831 23.925851008904388 33.963972247172734 30.16740810942705 3 21.858182409640257 31.069890458039563 25.95274632797528 21.119180804344907 4 13.304387180720164 21.952875286397195 28.675784233304103 36.066953299578536 5 10.509009136424066 40.2073374253953 34.83438463496733 14.449268803213306 6 33.12988868300825 29.208333922686318 18.937325846193016 18.72445154811242 7 22.162192741775804 33.43351342177468 23.68328006109807 20.721013775351455 8 29.70752128532232 31.25901620795972 21.797301501994173 17.236161004723787 9 29.085933301275706 14.900432777982067 18.967272932990127 37.0463609877521 10 18.172432325403783 30.42952507566543 30.45144683619495 20.946595762735836 11 27.168839985291203 26.640596271886402 17.962407716459705 28.228156026362683 12 26.151246004582358 31.11617118773513 27.6348541849348 15.097728622747717 13 32.28792464571607 19.671597884196533 21.698300002828613 26.342177467258786 14 22.800752411393656 27.1511611461545 25.16405962718864 24.8840268152632 15 36.87593697847424 19.236698441433543 22.588606341753177 21.298758238339037 16 16.86914830424575 26.170339150849998 35.641246853166635 21.31926569173762 17 26.493508330269 27.75012021610613 28.15178344129211 17.60458801233276 18 27.991259581930812 20.55200407320454 31.2059796905496 20.25075665431505 19 22.836110089667073 30.093627132068 32.438548355160805 14.631714423104123 20 24.923627414929424 23.676208525443386 29.660141996435947 21.74002206319124 21 18.805334766497893 27.618589652929032 33.17893813820609 20.397137442366983 22 22.12683506350239 26.6519107289339 22.09006307809804 29.131191129465673 23 16.625887477724664 27.18439736373151 29.738636042202927 26.451079116340903 24 21.39705258393913 35.12431759680933 24.83099029785308 18.647639521398467 25 21.72446468475094 22.305744915565864 23.442847848838856 32.52694255084434 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 552.0 1 552.0 2 494.5 3 437.0 4 437.0 5 437.0 6 867.0 7 1297.0 8 1297.0 9 1297.0 10 1171.0 11 1045.0 12 1045.0 13 1045.0 14 850.5 15 656.0 16 656.0 17 656.0 18 1002.5 19 1349.0 20 1349.0 21 1349.0 22 1958.5 23 2568.0 24 2568.0 25 2568.0 26 3429.5 27 4291.0 28 4291.0 29 4291.0 30 4710.0 31 5129.0 32 5129.0 33 5129.0 34 5295.5 35 5462.0 36 5462.0 37 5462.0 38 5315.0 39 5168.0 40 5168.0 41 5168.0 42 7067.0 43 8966.0 44 8966.0 45 8966.0 46 27363.0 47 45760.0 48 45760.0 49 45760.0 50 39015.0 51 32270.0 52 32270.0 53 32270.0 54 22313.0 55 12356.0 56 12356.0 57 12356.0 58 9044.0 59 5732.0 60 5732.0 61 5732.0 62 4945.0 63 4158.0 64 4158.0 65 4158.0 66 3272.0 67 2386.0 68 2386.0 69 2386.0 70 1769.0 71 1152.0 72 1152.0 73 1152.0 74 805.0 75 458.0 76 458.0 77 458.0 78 305.0 79 152.0 80 152.0 81 152.0 82 104.5 83 57.0 84 57.0 85 57.0 86 33.0 87 9.0 88 9.0 89 9.0 90 5.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04101490679716007 2 0.014850224874833819 3 0.0035357678273413855 4 0.0 5 0.0 6 0.009900149916555879 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 141412.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.95547761151812 #Duplication Level Percentage of deduplicated Percentage of total 1 84.10402763770573 27.71688399852912 2 9.490805312962685 6.255480440132379 3 2.9933695255670236 2.9594376714847397 4 1.2230972255005044 1.6123101292676716 5 0.5621955668090037 0.9263711707634429 6 0.33688818316417396 0.666138658671117 7 0.1523507070360277 0.3514553220377337 8 0.12445550715619166 0.32811925437728057 9 0.1330386455807566 0.3945916895312986 >10 0.6458811664485119 4.154527197126128 >50 0.06437353818423706 1.559273611857551 >100 0.09226873806407314 6.635929058354312 >500 0.034332553698259764 8.999236274149295 >1k 0.03862412291054224 24.317596809323113 >5k 0.0042915692122824705 13.122648714394819 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 9542 6.7476593216983005 No Hit GTACATGGGAAGCAGTGGTATCAAC 9015 6.374989392696517 No Hit GAGTACATGGGAAGCAGTGGTATCA 3928 2.7776992051593923 No Hit CATGTACTCTGCGTTGATACCACTG 3222 2.278448787938789 No Hit GCGTTGATACCACTGCTTCCCATGT 2912 2.059231182643623 No Hit TATCAACGCAGAGTACATGGGAAGC 2554 1.8060702062059797 No Hit GTATCAACGCAGAGTACATGGGAAG 2498 1.7664696065397563 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2462 1.7410120781828982 No Hit ACTCTGCGTTGATACCACTGCTTCC 2028 1.434107430769666 No Hit GTATCAACGCAGAGTACTTTTTTTT 1933 1.3669278420501796 No Hit GGTATCAACGCAGAGTACATGGGAA 1896 1.3407631601278533 No Hit GCTTCCCATGTACTCTGCGTTGATA 1465 1.035979973411026 No Hit TATCAACGCAGAGTACTTTTTTTTT 1371 0.9695075382570079 No Hit GGTATCAACGCAGAGTACTTTTTTT 1313 0.9284926314598478 No Hit GTACTCTGCGTTGATACCACTGCTT 1308 0.9249568636325064 No Hit ATACCACTGCTTCCCATGTACTCTG 1288 0.9108137923231409 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1118 0.7905976861935338 No Hit GTGGTATCAACGCAGAGTACATGGG 1069 0.7559471614855883 No Hit CAGTGGTATCAACGCAGAGTACATG 1012 0.7156394082538964 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1011 0.7149322546884281 No Hit TACCACTGCTTCCCATGTACTCTGC 983 0.6951319548553164 No Hit CATGGGAAGCAGTGGTATCAACGCA 974 0.6887675727661019 No Hit CTGCTTCCCATGTACTCTGCGTTGA 953 0.673917347891268 No Hit GCAGTGGTATCAACGCAGAGTACAT 944 0.6675529658020536 No Hit GATACCACTGCTTCCCATGTACTCT 903 0.6385596696178543 No Hit CCATGTACTCTGCGTTGATACCACT 880 0.6222951376120839 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 864 0.6109806805645914 No Hit GTTGATACCACTGCTTCCCATGTAC 779 0.5508726274997879 No Hit ACATGGGAAGCAGTGGTATCAACGC 776 0.548751166803383 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 765 0.540972477583232 No Hit CCACTGCTTCCCATGTACTCTGCGT 732 0.5176364099227788 No Hit AAGCAGTGGTATCAACGCAGAGTAC 704 0.4978361100896671 No Hit ATGGGAAGCAGTGGTATCAACGCAG 688 0.48652165304217465 No Hit CTTCCCATGTACTCTGCGTTGATAC 654 0.4624784318162532 No Hit ATCAACGCAGAGTACATGGGAAGCA 576 0.4073204537097276 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 551 0.38964161457302066 No Hit TGATACCACTGCTTCCCATGTACTC 477 0.3373122507283682 No Hit GTACATGGTAAGCAGTGGTATCAAC 476 0.3366050971628999 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 434 0.30690464741323226 No Hit TTCCCATGTACTCTGCGTTGATACC 432 0.3054903402822957 No Hit GGAAGCAGTGGTATCAACGCAGAGT 401 0.28356857975277916 No Hit ACCATGTACTCTGCGTTGATACCAC 378 0.2673040477470088 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 330 0.23336067660453141 No Hit CCCCATGTACTCTGCGTTGATACCA 313 0.22133906599157072 No Hit AAAAAGTACTCTGCGTTGATACCAC 305 0.21568183746782452 No Hit TCCATGTACTCTGCGTTGATACCAC 291 0.20578168755126863 No Hit TCAACGCAGAGTACATGGGAAGCAG 277 0.19588153763471275 No Hit GGTAAGCAGTGGTATCAACGCAGAG 260 0.18385992702175205 No Hit CTGCGTTGATACCACTGCTTCCCAT 258 0.18244561989081548 No Hit TGGGAAGCAGTGGTATCAACGCAGA 247 0.17466693067066444 No Hit ATCAACGCAGAGTACTTTTTTTTTT 240 0.1697168557123865 No Hit CTCTGCGTTGATACCACTGCTTCCC 237 0.16759539501598167 No Hit GTACATGGAAAGCAGTGGTATCAAC 230 0.16264532005770374 No Hit GTACATGGGTAAGCAGTGGTATCAA 225 0.15910955223036233 No Hit AAAGTACTCTGCGTTGATACCACTG 224 0.15840239866489406 No Hit TCCCATGTACTCTGCGTTGATACCA 215 0.15203801657567956 No Hit GAGTACATGGTAAGCAGTGGTATCA 184 0.13011625604616298 No Hit AACGCAGAGTACATGGGAAGCAGTG 166 0.117387491867734 No Hit GAGTACATGGAAAGCAGTGGTATCA 160 0.11314457047492434 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 159 0.11243741690945606 No Hit ATGTACTCTGCGTTGATACCACTGC 154 0.10890164908211467 No Hit ACTGCTTCCCATGTACTCTGCGTTG 153 0.1081944955166464 No Hit GTACATGGAAGCAGTGGTATCAACG 152 0.10748734195117812 No Hit AGTGGTATCAACGCAGAGTACATGG 152 0.10748734195117812 No Hit CAACGCAGAGTACATGGGAAGCAGT 152 0.10748734195117812 No Hit CGTTGATACCACTGCTTCCCATGTA 142 0.10041580629649535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 7.071535654682771E-4 0.0 11 0.0 0.0 0.0 7.071535654682771E-4 0.0 12 0.0 0.0 0.0 7.071535654682771E-4 0.0 13 0.0 0.0 0.0 7.071535654682771E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 60 0.0058164774 11.087048 1 CATGGTA 190 1.0113581E-8 8.999833 4 GAAGCAG 2235 0.0 8.75599 9 GGAAGCA 2315 0.0 8.41237 8 CATGGGT 125 2.697052E-4 8.359844 4 AGTGGTA 2530 0.0 8.3358135 14 CAGTGGT 2570 0.0 8.206073 13 TGGTATC 2580 0.0 8.174266 16 GTGGTAT 2580 0.0 8.174266 15 AGCAGTG 2630 0.0 8.127226 11 ACATGGT 200 2.2168024E-7 8.07485 3 TGGTAAG 200 2.2168024E-7 8.07485 6 AAGCAGT 2650 0.0 8.065887 10 GGGAAGC 2440 0.0 7.9814086 7 ATGGTAA 220 1.2184319E-7 7.7725825 5 GCAGTGG 2775 0.0 7.7025595 12 CCATGTA 2465 0.0 7.592151 2 TGCGTTG 2670 0.0 7.3650317 12 TGGGAAG 2655 0.0 7.33508 6 TACATGG 3370 0.0 7.272862 2 >>END_MODULE