FastQCFastQC Report
Wed 25 May 2016
SRR1294877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451598
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC100552.226537761460414No Hit
CCCATGTACTCTGCGTTGATACCAC97742.1643142795140813No Hit
GAGTACATGGGAAGCAGTGGTATCA48191.0670994999977856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47281.0469488350258416No Hit
CATGTACTCTGCGTTGATACCACTG43740.968560533926191No Hit
GCGTTGATACCACTGCTTCCCATGT30850.6831296861367854No Hit
ACGCAGAGTACATGGGAAGCAGTGG27660.6124916407955748No Hit
TATCAACGCAGAGTACATGGGAAGC25250.5591255940017449No Hit
ACTCTGCGTTGATACCACTGCTTCC24820.5496038512128043No Hit
GTATCAACGCAGAGTACATGGGAAG23750.5259102121798591No Hit
GGTATCAACGCAGAGTACATGGGAA22020.48760180514528406No Hit
GTGGTATCAACGCAGAGTACATGGG18470.40899206816682093No Hit
GTATCAACGCAGAGTACTTTTTTTT16780.3715694046474962No Hit
GCTTCCCATGTACTCTGCGTTGATA15680.3472114579781133No Hit
GTACTCTGCGTTGATACCACTGCTT15200.3365825357951098No Hit
ATACCACTGCTTCCCATGTACTCTG13440.29760982112409706No Hit
GGGAAGCAGTGGTATCAACGCAGAG12970.2872023348199062No Hit
CAGTGGTATCAACGCAGAGTACATG12560.2781234637885907No Hit
GCAGAGTACATGGGAAGCAGTGGTA12340.27325187445471416No Hit
GATACCACTGCTTCCCATGTACTCT12160.26926602863608784No Hit
GGTATCAACGCAGAGTACTTTTTTT12150.26904459275727527No Hit
CTGCTTCCCATGTACTCTGCGTTGA12080.2674945416055873No Hit
GCAGTGGTATCAACGCAGAGTACAT11680.2586371064530844No Hit
GTTGATACCACTGCTTCCCATGTAC11420.25287977360395747No Hit
TATCAACGCAGAGTACTTTTTTTTT11030.2442437743302672No Hit
CATGGGAAGCAGTGGTATCAACGCA11030.2442437743302672No Hit
TACCACTGCTTCCCATGTACTCTGC10160.2249788528735734No Hit
CCACTGCTTCCCATGTACTCTGCGT10150.22475741699476082No Hit
AAGCAGTGGTATCAACGCAGAGTAC9210.20394244438637904No Hit
CTTCCCATGTACTCTGCGTTGATAC8440.18689188171781096No Hit
ACATGGGAAGCAGTGGTATCAACGC8330.18445608705087269No Hit
ATGGGAAGCAGTGGTATCAACGCAG7270.16098388389674004No Hit
TGATACCACTGCTTCCCATGTACTC6100.13507588607566906No Hit
CCATGTACTCTGCGTTGATACCACT6030.13352583492398107No Hit
GTACATGGTAAGCAGTGGTATCAAC5680.12577557916554105No Hit
TTCCCATGTACTCTGCGTTGATACC5640.12488983565029076No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5560.12311834861979017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5070.11226799055797414No Hit
ATCAACGCAGAGTACATGGGAAGCA4720.1045177347995341No Hit
ACCATGTACTCTGCGTTGATACCAC4590.10163906837497066No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4550.10075332485972037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACC250.006030667618.99722912
TCTTCGC250.006030667618.9972298
AGGCCTC350.002167495716.28514517
TCAACGG350.002167495716.2851455
TCCCGAC400.005272642214.2495012
CAAGACG500.001497066313.2995344
GTTATTC500.001497066313.2995343
AAGACGG550.00306344712.0904865
CCGGAAT802.8568282E-511.8745853
GTAAAAA907.3030988E-611.6287551
ATTCCCC907.43055E-611.61070419
GACGGAC600.00587107811.0817177
TTACCCG600.00587107811.08171713
ATCGAAC909.493877E-510.5540168
CGAACCC1002.3918803E-510.44847610
AATCGAA1002.3918803E-510.4484767
TAAATGA750.002642112810.1329784
GAACCCT951.6403702E-49.99854111
CCCTGAT1002.7460756E-49.49966714
GATTCCC1002.7460756E-49.49966718
Produced by FastQC (version 0.11.5)