##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294877_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451598
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.454390852040973	31.0	31.0	34.0	26.0	34.0
2	30.600286095155425	31.0	31.0	34.0	26.0	34.0
3	30.713078888746185	31.0	31.0	34.0	26.0	34.0
4	34.0491764799667	37.0	35.0	37.0	28.0	37.0
5	34.517267569829805	37.0	35.0	37.0	31.0	37.0
6	34.532252135749054	37.0	35.0	37.0	30.0	37.0
7	34.61460192472066	37.0	35.0	37.0	31.0	37.0
8	34.58950438221604	37.0	35.0	37.0	30.0	37.0
9	36.00429142733139	39.0	37.0	39.0	30.0	39.0
10	36.143953250457265	39.0	37.0	39.0	30.0	39.0
11	36.15470396237362	39.0	37.0	39.0	30.0	39.0
12	36.14831553726987	39.0	37.0	39.0	30.0	39.0
13	35.95388819259607	39.0	37.0	39.0	30.0	39.0
14	37.15059189810407	40.0	38.0	41.0	30.0	41.0
15	37.2185040677771	40.0	38.0	41.0	30.0	41.0
16	37.11834640543138	40.0	37.0	41.0	30.0	41.0
17	37.14886248389054	40.0	38.0	41.0	30.0	41.0
18	37.072801473877206	40.0	37.0	41.0	30.0	41.0
19	37.12370958241622	40.0	37.0	41.0	30.0	41.0
20	37.15787935287579	40.0	38.0	41.0	30.0	41.0
21	37.08623377428598	40.0	37.0	41.0	29.0	41.0
22	37.1319934986426	40.0	37.0	41.0	30.0	41.0
23	37.09324000549161	40.0	37.0	41.0	29.0	41.0
24	37.14884919773781	40.0	37.0	41.0	29.0	41.0
25	36.049721212228576	40.0	35.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.04117170938518555
1101	2	-0.06202596341464428
1101	3	-0.1440736256722559
1101	4	-0.2755851196667294
1101	5	-0.1811603581083574
1101	6	-0.13386581636137151
1101	7	-0.23043055882995844
1101	8	-0.2531215321987972
1101	9	-0.12723958599268315
1101	10	0.2445622434781214
1101	11	0.15331255453479287
1101	12	0.1145046042760427
1101	13	-0.0756204817993762
1101	14	0.14449096069896683
1101	15	0.3345289892252481
1101	16	0.3134476978875185
1101	17	0.0523782557038075
1101	18	0.3784719171079729
1101	19	0.032817383440715275
1101	20	-0.18516369263493004
1101	21	-0.16291871894226517
1101	22	0.005701883815937947
1101	23	-0.05792880960422053
1101	24	-0.06953946768381059
1101	25	0.16882100219918073
1102	1	-0.5761973760935888
1102	2	-0.5495922971685303
1102	3	-0.6861155014126403
1102	4	-0.7467088688360519
1102	5	-0.595398398770584
1102	6	-0.5466592017665022
1102	7	-0.472398275600483
1102	8	-0.4789042945722741
1102	9	-0.2506107371902999
1102	10	-0.2012704076568923
1102	11	-0.4039632108917175
1102	12	-0.15123009923254216
1102	13	-0.22207000072917538
1102	14	-0.3432776247909146
1102	15	-0.17972494264481043
1102	16	-0.13586657241710753
1102	17	-0.35511324490776275
1102	18	-0.3134120096576325
1102	19	-0.42110292937530147
1102	20	-0.6767822843849913
1102	21	-0.5977888444346533
1102	22	-0.5433850940615699
1102	23	-0.670551725689009
1102	24	-0.6590647539663266
1102	25	-0.42615063440260315
1103	1	-0.7060142575289419
1103	2	-0.4572125146722179
1103	3	-0.8159964103181494
1103	4	-1.0322761285077533
1103	5	-0.9929734176231193
1103	6	-0.7683578742636215
1103	7	-0.6109608351816007
1103	8	-0.5393011700317274
1103	9	-0.7449763198165655
1103	10	-0.660276227042516
1103	11	-0.5586867782177833
1103	12	-0.5931761549032402
1103	13	-0.7873139928182269
1103	14	-0.7656715881861729
1103	15	-0.5485729698873172
1103	16	-0.5846256251899717
1103	17	-0.7803789589089121
1103	18	-0.5925954934159989
1103	19	-0.7918077532523284
1103	20	-0.49466918815881655
1103	21	-0.7162799778157307
1103	22	-0.39533698334621903
1103	23	-0.6765181373524456
1103	24	-0.8232881007533308
1103	25	-0.6766000123875884
1104	1	-0.2868288213879424
1104	2	-0.18938476137027394
1104	3	-0.3566214532973042
1104	4	-0.30069985941928223
1104	5	-0.2515612257550046
1104	6	-0.18822639243406059
1104	7	-0.3180447167379299
1104	8	-0.22795559012259048
1104	9	-0.19288329229615186
1104	10	-0.02069139421423216
1104	11	-0.027780754717930733
1104	12	0.025370668306877064
1104	13	-0.17030754250671265
1104	14	-0.133914692037564
1104	15	-0.08721524120382895
1104	16	0.1493093398994887
1104	17	-0.07296270522416393
1104	18	-0.13350477187049137
1104	19	-0.2787627202672667
1104	20	-0.012060273250661169
1104	21	-0.288150293087881
1104	22	-0.3312350069927774
1104	23	-0.2951887405276281
1104	24	-0.25874601785743323
1104	25	-0.49562224235242525
1105	1	-0.14913301188636652
1105	2	-0.13414488743540787
1105	3	-0.12074658461515142
1105	4	-0.14237605878895465
1105	5	-0.14626182106258057
1105	6	0.016606104355943785
1105	7	-0.17384254561618206
1105	8	-0.11821355673868084
1105	9	-0.04756521128763325
1105	10	-0.2680521972958303
1105	11	-0.2625817352245292
1105	12	-0.2108847303157475
1105	13	-0.11126937278569216
1105	14	-0.0744236216511922
1105	15	-0.29451301171859257
1105	16	-0.36669859753484957
1105	17	-0.30845704396584495
1105	18	-0.012344505732990285
1105	19	-0.28813589640827075
1105	20	-0.08058134246976323
1105	21	-0.04158790251942435
1105	22	0.08923656998866392
1105	23	-0.09777307576750616
1105	24	-0.04616051534780041
1105	25	-0.010357855752680223
1106	1	-0.12954748325974208
1106	2	-0.44377139487013295
1106	3	-0.4392128063690457
1106	4	-0.18116308606913378
1106	5	-0.4425151871789623
1106	6	-0.3074074771918802
1106	7	-0.22884078938155028
1106	8	-0.23139468307049071
1106	9	-0.21471425706681657
1106	10	0.02842596685356824
1106	11	-0.43827310625836446
1106	12	-0.08525541290560312
1106	13	-0.2834904241848264
1106	14	-0.35286642694331505
1106	15	-0.5403671271617512
1106	16	-0.8056729565092056
1106	17	-0.2781280426779418
1106	18	-0.07574925927472975
1106	19	-0.1269252653346271
1106	20	-0.29923723756401444
1106	21	-0.23089828772021548
1106	22	-0.5601183064894997
1106	23	-0.14354991688681196
1106	24	-0.021580255425078576
1106	25	-0.10870465355153414
1107	1	-0.20662195972014175
1107	2	-0.13918025436732506
1107	3	-0.044327486471917865
1107	4	-0.12025404287383168
1107	5	-0.08927685200864488
1107	6	-0.21683775063963395
1107	7	-0.04107508319354736
1107	8	0.038280696134883385
1107	9	-0.16508952605332894
1107	10	-0.2922387578616963
1107	11	0.08476132401508352
1107	12	-0.0621225729328998
1107	13	-0.11904125607481575
1107	14	-0.24275253411803988
1107	15	-0.4250965660952559
1107	16	-0.18498330133679275
1107	17	-0.34568958154321194
1107	18	-0.03147801590097998
1107	19	-0.16598735529421305
1107	20	-0.3123585307986758
1107	21	-0.19600876900925357
1107	22	-0.25226652461788035
1107	23	-0.026645754812598454
1107	24	-0.36310127573683815
1107	25	-0.5535099335599298
1108	1	0.2100934114078541
1108	2	0.1675847953112246
1108	3	0.18000526240788162
1108	4	0.34954919110239757
1108	5	0.1915043675510617
1108	6	0.3201045832781517
1108	7	0.33185497719998835
1108	8	0.1673231664251631
1108	9	0.2766112337362685
1108	10	0.21854928032259835
1108	11	0.12404147207664806
1108	12	0.20219653869772714
1108	13	0.20942163112565026
1108	14	0.2903177951241176
1108	15	0.4610324033183417
1108	16	0.30873382315181175
1108	17	0.2814777669859154
1108	18	0.441831939290914
1108	19	0.27483672419146643
1108	20	0.5049510347575321
1108	21	0.4525885425044791
1108	22	0.2990192731611998
1108	23	0.3603842471355847
1108	24	0.14868520390469797
1108	25	0.19008542977535114
1109	1	0.023216373145945823
1109	2	-0.13557077861739586
1109	3	0.12016648605401414
1109	4	0.020900989366140266
1109	5	-0.15462121234880044
1109	6	0.06709599387022536
1109	7	0.027516637206417727
1109	8	-0.18493442394734672
1109	9	-0.056641717437308614
1109	10	-0.2018149656927406
1109	11	-0.16613812463457123
1109	12	-0.16648197859591818
1109	13	-0.06290164295454304
1109	14	0.005822365424059228
1109	15	-0.038473820557456406
1109	16	0.07779587817913836
1109	17	-0.08942848444948481
1109	18	-0.045215049823042364
1109	19	-0.03541544612684078
1109	20	0.1372256202152542
1109	21	0.24196431761816228
1109	22	0.11167142510644368
1109	23	-0.013691358027251965
1109	24	0.09923869249043094
1109	25	0.0022208392650426845
1110	1	0.2931395703032287
1110	2	0.25629239312635477
1110	3	0.3092161773808044
1110	4	0.4155641476159815
1110	5	0.31360625000881015
1110	6	0.22486230182376232
1110	7	0.01608617020465175
1110	8	0.11686251518386825
1110	9	0.1016868368025925
1110	10	0.2024850939053664
1110	11	0.13707567513827712
1110	12	0.11776725393789889
1110	13	0.19699108045401914
1110	14	0.15410920098950953
1110	15	0.23652646056569182
1110	16	0.02005318456963323
1110	17	-0.05996677904404635
1110	18	0.30321459629914216
1110	19	0.1579860314563959
1110	20	0.15752271115296423
1110	21	0.15629485096498996
1110	22	0.30229540603505
1110	23	0.38799987880213394
1110	24	0.28181692906117917
1110	25	0.15367874388557823
1111	1	0.18577096264377602
1111	2	0.1816977745754791
1111	3	0.21455792396038476
1111	4	0.1170508694230179
1111	5	0.08829707798229691
1111	6	0.05421522420805758
1111	7	-0.007382650371241084
1111	8	0.12141261148562421
1111	9	-0.020076251649143728
1111	10	-0.23051086012831945
1111	11	-0.17036236580889863
1111	12	-0.19938221414032853
1111	13	-0.12858271365043095
1111	14	0.01869951689250371
1111	15	-0.14404366797822377
1111	16	-0.048218572886597144
1111	17	0.006761195738377523
1111	18	-0.02074339656178381
1111	19	-0.24548415562568948
1111	20	-0.35066247628920877
1111	21	-0.024673041679328378
1111	22	-0.14173600883438553
1111	23	-0.10987742002137679
1111	24	-0.11162421053112581
1111	25	-0.0833639151138783
1112	1	0.3622831409489713
1112	2	0.30829320010123595
1112	3	0.3180020255132199
1112	4	0.23370399781672546
1112	5	-0.01332944463480601
1112	6	0.28404772974763404
1112	7	0.2996215198846741
1112	8	0.10875096362926229
1112	9	0.033779173630996695
1112	10	0.10181134258085223
1112	11	0.09490942013785286
1112	12	0.1538175844522769
1112	13	0.14167513343083016
1112	14	0.31209816142774827
1112	15	0.13717403973192432
1112	16	0.1027808323650774
1112	17	0.06300964965590339
1112	18	0.09223246788472039
1112	19	0.07916421478146418
1112	20	0.050276840083562035
1112	21	0.3014121985621472
1112	22	0.21566737965206073
1112	23	-0.034954921517019955
1112	24	0.04386475504540499
1112	25	0.06883551329614335
1113	1	0.2867579326121721
1113	2	0.14751041928318998
1113	3	0.1455241067188311
1113	4	0.15298908239897457
1113	5	0.20719484884978812
1113	6	0.04883589987687742
1113	7	0.20124995681809565
1113	8	0.16634340528663927
1113	9	-0.1809050360198654
1113	10	0.1619770139992056
1113	11	0.1961719161873532
1113	12	-0.06307557744329984
1113	13	-0.09606002517127621
1113	14	-0.004472307142918908
1113	15	-0.058466273646544664
1113	16	-0.12128477085519762
1113	17	-0.16855441494929124
1113	18	-0.14669698869306558
1113	19	0.05782320777868222
1113	20	-0.05670597732856919
1113	21	-0.18910494244152432
1113	22	0.2936377384404665
1113	23	0.1231032630835216
1113	24	0.012047858556776703
1113	25	-0.03475902420448307
1114	1	0.5255530265254151
1114	2	0.6185389617038091
1114	3	0.5398385482338881
1114	4	0.5446654378773843
1114	5	0.544085477176047
1114	6	0.6775076866029366
1114	7	0.46772775988685567
1114	8	0.5453828210103495
1114	9	0.6681099802737194
1114	10	0.6243331146755153
1114	11	0.5731611247615405
1114	12	0.5853138964132825
1114	13	0.5755989723232773
1114	14	0.45149736551170605
1114	15	0.49892315108367313
1114	16	0.4948876299504761
1114	17	0.7061274918088571
1114	18	0.10079511014919262
1114	19	0.6757510575174948
1114	20	0.6457536162325965
1114	21	0.6186668491971687
1114	22	0.7534359928168044
1114	23	0.834536606787978
1114	24	0.61108498171658
1114	25	0.5681852828680789
1115	1	0.4201137621458422
1115	2	0.381825903801964
1115	3	0.4462895262180986
1115	4	0.4204167790623998
1115	5	0.315566727501988
1115	6	0.4016416150091402
1115	7	0.19901131054066212
1115	8	0.30035601413258206
1115	9	0.06181360235645883
1115	10	-0.10372890030673432
1115	11	0.1206472334660802
1115	12	0.10010856767365084
1115	13	0.05838352308307293
1115	14	0.2091554520709238
1115	15	-0.007054774247350792
1115	16	0.3096283005345697
1115	17	0.02671150659932664
1115	18	-0.21152225905898803
1115	19	0.41870615620751295
1115	20	0.2703059237100902
1115	21	0.5638637329712282
1115	22	0.42389896870827926
1115	23	0.17234195874284808
1115	24	0.18860791518135755
1115	25	0.3921969106772494
1116	1	0.299972848423824
1116	2	0.02073801045154866
1116	3	0.28816983049882694
1116	4	-0.0116963460794679
1116	5	0.1953536676435732
1116	6	0.2814338341363154
1116	7	-0.20204906169820447
1116	8	-0.11648148637331701
1116	9	-0.15402300248155143
1116	10	-0.15439132501519737
1116	11	-0.24058319249526505
1116	12	-0.24492241117345515
1116	13	-0.25901372586157123
1116	14	-0.4450762707612199
1116	15	-0.07797090700328368
1116	16	-0.13629992989666562
1116	17	-0.15855801200998343
1116	18	-0.18873463792826328
1116	19	0.19175376737855032
1116	20	0.10838711802779244
1116	21	-0.16850577490414054
1116	22	0.031489094189765865
1116	23	-0.1992053647234755
1116	24	-0.016142819917384088
1116	25	-0.4201069970038489
1201	1	-0.11473910857605318
1201	2	0.02677914209543175
1201	3	-0.3848046936887428
1201	4	-0.16914909998924088
1201	5	-0.614516167335502
1201	6	-0.5527199256511963
1201	7	-0.6602345437109634
1201	8	-0.5610618539523031
1201	9	-0.680854089591989
1201	10	-0.2981593656692496
1201	11	-0.5092166482990663
1201	12	-0.3708037586558248
1201	13	-0.3132603230119315
1201	14	-0.4079601779741324
1201	15	-0.33982380000151835
1201	16	-0.3897802836505875
1201	17	-0.4640750482525817
1201	18	-0.3368408143779007
1201	19	-0.414332609911483
1201	20	-0.3617339978639009
1201	21	-0.4158674928980872
1201	22	-0.18796276318590088
1201	23	-0.3128766718618081
1201	24	-0.3890612059884333
1201	25	-0.2420130117422019
1202	1	-0.22179755164767911
1202	2	-0.10878272271840572
1202	3	-0.3213681136237767
1202	4	-0.28511976669395267
1202	5	-0.4294829010578525
1202	6	-0.21773160094949873
1202	7	-0.20218001459880952
1202	8	-0.36779881019489835
1202	9	-0.43462181060229454
1202	10	-0.20489965091747564
1202	11	-0.2701369756829948
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2209	21	0.4598026724722999
2209	22	0.21704365529933511
2209	23	0.42086945816915033
2209	24	0.3961460733289428
2209	25	0.5030976127386566
2210	1	0.3451572122533797
2210	2	0.3615764637168617
2210	3	0.3621893303590653
2210	4	0.40479430846349373
2210	5	0.2210011485259713
2210	6	0.26713751548927434
2210	7	0.35931329990628313
2210	8	0.37169068402739924
2210	9	0.38077741735595083
2210	10	0.3230516646425059
2210	11	0.4249758781607227
2210	12	0.287378975882298
2210	13	0.3755221667281461
2210	14	0.5236043695094921
2210	15	0.4585418130735732
2210	16	0.33294993450766697
2210	17	0.26023915723377655
2210	18	0.2836862214058087
2210	19	0.5279958514608225
2210	20	0.40149589629694304
2210	21	0.1412280503375669
2210	22	0.1250547326741298
2210	23	0.2649187057751661
2210	24	0.2673215429495741
2210	25	0.49155311972874927
2211	1	0.5351095422169116
2211	2	0.4643680759243516
2211	3	0.6882870558391012
2211	4	0.4621107635808741
2211	5	0.6119125950505548
2211	6	0.45022513156828836
2211	7	0.38682169265251076
2211	8	0.5145998137348613
2211	9	0.5064437880695536
2211	10	0.8707178905550421
2211	11	0.6615751107150487
2211	12	0.4788517170808717
2211	13	0.5283749383349416
2211	14	0.3585825921906718
2211	15	0.5015915522411234
2211	16	0.6479017881518843
2211	17	0.6205080299574419
2211	18	0.5247285604765679
2211	19	0.6852517187620748
2211	20	0.48852514992363893
2211	21	0.42963558151743086
2211	22	0.2552575684887728
2211	23	0.6672237101763585
2211	24	0.6192091011081615
2211	25	0.3197771346051539
2212	1	0.5074815500223124
2212	2	0.6827740759541427
2212	3	0.5981674425341694
2212	4	0.6172989720625495
2212	5	0.6238739255437551
2212	6	0.2903000574616641
2212	7	0.5669678204883013
2212	8	0.4672944646516939
2212	9	0.6470378327271789
2212	10	0.4645763506268352
2212	11	0.42724549636741216
2212	12	0.35668005783728063
2212	13	0.5249749711057135
2212	14	0.6246179250374126
2212	15	0.48708303928921737
2212	16	0.600298522991686
2212	17	0.6353479745450556
2212	18	0.6170713725064232
2212	19	0.5658953664465258
2212	20	0.5715618084969165
2212	21	0.5623199543296096
2212	22	0.5601374338307039
2212	23	0.47701919078946986
2212	24	0.5515523600121313
2212	25	0.8845701574106286
2213	1	0.37410968496999786
2213	2	0.23733393597508368
2213	3	0.5716156917418687
2213	4	0.36773816338834564
2213	5	0.3151856670603337
2213	6	0.17103853132451263
2213	7	0.18004201922764906
2213	8	0.095610359161455
2213	9	0.05743226167304982
2213	10	0.05319110604997235
2213	11	0.12513281041380253
2213	12	0.18009879338788437
2213	13	0.12327828717589995
2213	14	0.19777490255783903
2213	15	0.15688494643363526
2213	16	0.09621053013118086
2213	17	0.2482921726519649
2213	18	0.13165428128813517
2213	19	-0.04829964855625235
2213	20	0.3314857756945955
2213	21	0.16474991209697265
2213	22	0.05798012603839453
2213	23	0.1161620298627426
2213	24	0.2882799981465851
2213	25	0.42888807571401344
2214	1	0.11824631948239528
2214	2	0.04108933672880255
2214	3	0.2746380307694629
2214	4	0.2854991571443861
2214	5	0.2852695273776149
2214	6	0.44030288313859245
2214	7	0.2727848296505613
2214	8	0.26836504299711805
2214	9	0.38281584754785314
2214	10	0.06618416079786016
2214	11	0.3037651807392976
2214	12	0.3917979238860667
2214	13	0.20445667131551915
2214	14	0.4152440950442653
2214	15	0.36631007536605154
2214	16	0.3220240932486078
2214	17	0.2888440433220296
2214	18	0.5226072268145145
2214	19	0.472752224717631
2214	20	0.6802620677527784
2214	21	0.6588592065142151
2214	22	0.7829526557523039
2214	23	0.45552638566934434
2214	24	0.4082587509869171
2214	25	0.1740766529354616
2215	1	0.5214797651670899
2215	2	0.6152162598918416
2215	3	0.7166197778359553
2215	4	0.6107399777169107
2215	5	0.7127994566427631
2215	6	0.6804913716310423
2215	7	0.624879749038314
2215	8	0.49305111079717534
2215	9	0.6161375266523947
2215	10	0.5104875680235992
2215	11	0.4460753703817346
2215	12	0.639180458732497
2215	13	0.660855790186929
2215	14	0.7395789859107325
2215	15	0.8057738200936626
2215	16	0.9027147010309946
2215	17	0.7560540381723513
2215	18	0.8064560157793963
2215	19	0.6196867893805162
2215	20	0.9728997527062475
2215	21	1.085791497741333
2215	22	0.7852209246170858
2215	23	0.5981069763837326
2215	24	0.6406452212884446
2215	25	0.7898246735823946
2216	1	0.7659195473041223
2216	2	0.7469947137041935
2216	3	0.8672306233620546
2216	4	0.8484560465259108
2216	5	0.7807742335596828
2216	6	0.6045224026170715
2216	7	0.42188787976941455
2216	8	0.6227315262753734
2216	9	0.6912323130271361
2216	10	0.6475760569531133
2216	11	0.5201061516012899
2216	12	0.6182733402216343
2216	13	0.36275951020871133
2216	14	0.4622283087938399
2216	15	0.3312891299456169
2216	16	0.5646974138611611
2216	17	0.6667625257364804
2216	18	0.7865296469342695
2216	19	0.9320330789331166
2216	20	0.8702723759701669
2216	21	0.9633143472104138
2216	22	0.8441574855992329
2216	23	0.7139353435271261
2216	24	0.8936037051060737
2216	25	0.8048872567338137
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125.0
3	96.0
4	20.0
5	27.0
6	24.0
7	42.0
8	43.0
9	177.0
10	291.0
11	599.0
12	962.0
13	1459.0
14	2166.0
15	2821.0
16	3463.0
17	3656.0
18	3564.0
19	3183.0
20	2999.0
21	2873.0
22	2656.0
23	2559.0
24	2496.0
25	2534.0
26	2591.0
27	2670.0
28	3096.0
29	3586.0
30	4375.0
31	5693.0
32	7896.0
33	10806.0
34	15788.0
35	23881.0
36	38656.0
37	76975.0
38	216750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48714054160726	19.60438234909344	10.820171577703325	25.08830553159597
2	16.058780771037775	23.669210789919244	33.64868937762058	26.623319061422407
3	20.091678279691866	28.71249399039793	26.650286577706094	24.545541152204112
4	14.465845117248104	20.335121237643513	30.111485438184317	35.08754820692407
5	13.497953512490554	39.33156200070469	32.276221521469836	14.894262965334917
6	32.21120605598596	35.04640381512933	16.561552644175368	16.180837484709347
7	25.932647711232104	34.31811181357461	20.545173721217278	19.204066753976008
8	27.16681662448641	34.166670360209025	19.987235117609774	18.67927789769479
9	26.52930070116033	18.35493249891413	18.47326992456543	36.64249687536011
10	16.987433049020197	31.225471620762175	29.455550311015973	22.331545019201663
11	31.8384179513942	26.74316307785039	19.124102795930533	22.29431617482488
12	23.303645231990462	30.17083770086006	26.28671215542707	20.238804911722404
13	28.668114528853827	23.493706231717045	22.64936619094052	25.188813048488612
14	21.900787987838928	26.75701787819428	23.56229890355348	27.779895230413317
15	28.461424256726964	27.82523527469581	20.94260560493351	22.770734863643717
16	21.645182806707307	29.600748112544846	26.219173043129484	22.53489603761836
17	24.51936608403309	29.874542081923288	24.284007827545782	21.32208400649784
18	24.715453280265223	26.503621100779178	26.54395404703442	22.236971571921178
19	23.550242097215605	29.77341473413626	26.47867660908779	20.19766655956035
20	24.6971503543773	26.856879440797492	25.225946003448474	23.22002420137673
21	22.8526913939222	27.992136803659445	26.678575227828798	22.476596574589554
22	24.39092498731209	27.58007787827143	22.829148724449656	25.199848409966823
23	21.69861082791819	27.447230077747538	26.023705463604045	24.830453630730222
24	23.381090857507626	30.57242534931555	23.8110858926165	22.235397900560326
25	23.699223390485646	26.001347541623076	23.293409457614498	27.006019610276777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5351.0
1	5351.0
2	4172.0
3	2993.0
4	2993.0
5	2993.0
6	3138.0
7	3283.0
8	3283.0
9	3283.0
10	3389.0
11	3495.0
12	3495.0
13	3495.0
14	3552.5
15	3610.0
16	3610.0
17	3610.0
18	4615.0
19	5620.0
20	5620.0
21	5620.0
22	7276.5
23	8933.0
24	8933.0
25	8933.0
26	11250.0
27	13567.0
28	13567.0
29	13567.0
30	16204.5
31	18842.0
32	18842.0
33	18842.0
34	21895.0
35	24948.0
36	24948.0
37	24948.0
38	27419.0
39	29890.0
40	29890.0
41	29890.0
42	34499.0
43	39108.0
44	39108.0
45	39108.0
46	61157.0
47	83206.0
48	83206.0
49	83206.0
50	76274.0
51	69342.0
52	69342.0
53	69342.0
54	57063.5
55	44785.0
56	44785.0
57	44785.0
58	39170.0
59	33555.0
60	33555.0
61	33555.0
62	29608.0
63	25661.0
64	25661.0
65	25661.0
66	21221.0
67	16781.0
68	16781.0
69	16781.0
70	12888.5
71	8996.0
72	8996.0
73	8996.0
74	7110.0
75	5224.0
76	5224.0
77	5224.0
78	3781.5
79	2339.0
80	2339.0
81	2339.0
82	1777.0
83	1215.0
84	1215.0
85	1215.0
86	745.5
87	276.0
88	276.0
89	276.0
90	325.0
91	374.0
92	374.0
93	374.0
94	257.0
95	140.0
96	140.0
97	140.0
98	102.0
99	64.0
100	64.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13441157843923135
2	0.08015978813015115
3	0.052480303278579624
4	0.0925601973436552
5	0.07462389115983685
6	0.07573107055389972
7	0.07285240412933626
8	0.07971691637252601
9	0.07661681406915
10	0.07418101940221171
11	0.07418101940221171
12	0.07772399346321286
13	0.07927404461490085
14	0.07130235297764825
15	0.0701951735835854
16	0.07285240412933626
17	0.08215271103946431
18	0.07838830109965057
19	0.07373814764458655
20	0.08503137746402774
21	0.0845885057064026
22	0.08436706982759003
23	0.08724573625215346
24	0.09521742788940607
25	0.08990296679790433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	451598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.86453676188932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95839778856687	37.60536457875527
2	13.37219244856642	13.06851978980065
3	4.451750467156755	6.525981730714177
4	1.8568476337151145	3.6293599783559767
5	0.934984428892046	2.2843790498694734
6	0.544121662185059	1.5952951778869282
7	0.34217178539299575	1.1704046050352142
8	0.23538535821119572	0.9201597191617167
9	0.16580618751698437	0.7291838290745158
>10	1.0034329411307352	9.064331828316538
>50	0.07605624891243797	2.530285336721268
>100	0.04164985059471417	3.6553973512706897
>500	0.0045271576733384965	1.4894571753876513
>1k	0.011770609950680092	11.34535878463257
>5k	4.527157673338497E-4	2.1621794790195907
>10k+	4.527157673338497E-4	2.2243415859977476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	10055	2.226537761460414	No Hit
CCCATGTACTCTGCGTTGATACCAC	9774	2.1643142795140813	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4819	1.0670994999977856	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4728	1.0469488350258416	No Hit
CATGTACTCTGCGTTGATACCACTG	4374	0.968560533926191	No Hit
GCGTTGATACCACTGCTTCCCATGT	3085	0.6831296861367854	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2766	0.6124916407955748	No Hit
TATCAACGCAGAGTACATGGGAAGC	2525	0.5591255940017449	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2482	0.5496038512128043	No Hit
GTATCAACGCAGAGTACATGGGAAG	2375	0.5259102121798591	No Hit
GGTATCAACGCAGAGTACATGGGAA	2202	0.48760180514528406	No Hit
GTGGTATCAACGCAGAGTACATGGG	1847	0.40899206816682093	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1678	0.3715694046474962	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1568	0.3472114579781133	No Hit
GTACTCTGCGTTGATACCACTGCTT	1520	0.3365825357951098	No Hit
ATACCACTGCTTCCCATGTACTCTG	1344	0.29760982112409706	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1297	0.2872023348199062	No Hit
CAGTGGTATCAACGCAGAGTACATG	1256	0.2781234637885907	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1234	0.27325187445471416	No Hit
GATACCACTGCTTCCCATGTACTCT	1216	0.26926602863608784	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1215	0.26904459275727527	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1208	0.2674945416055873	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1168	0.2586371064530844	No Hit
GTTGATACCACTGCTTCCCATGTAC	1142	0.25287977360395747	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1103	0.2442437743302672	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1103	0.2442437743302672	No Hit
TACCACTGCTTCCCATGTACTCTGC	1016	0.2249788528735734	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1015	0.22475741699476082	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	921	0.20394244438637904	No Hit
CTTCCCATGTACTCTGCGTTGATAC	844	0.18689188171781096	No Hit
ACATGGGAAGCAGTGGTATCAACGC	833	0.18445608705087269	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	727	0.16098388389674004	No Hit
TGATACCACTGCTTCCCATGTACTC	610	0.13507588607566906	No Hit
CCATGTACTCTGCGTTGATACCACT	603	0.13352583492398107	No Hit
GTACATGGTAAGCAGTGGTATCAAC	568	0.12577557916554105	No Hit
TTCCCATGTACTCTGCGTTGATACC	564	0.12488983565029076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	556	0.12311834861979017	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	507	0.11226799055797414	No Hit
ATCAACGCAGAGTACATGGGAAGCA	472	0.1045177347995341	No Hit
ACCATGTACTCTGCGTTGATACCAC	459	0.10163906837497066	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	455	0.10075332485972037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.2143587881257224E-4	0.0	0.0	0.0	0.0
9	2.2143587881257224E-4	0.0	0.0	0.0	0.0
10	2.2143587881257224E-4	0.0	0.0	0.0	0.0
11	2.2143587881257224E-4	0.0	0.0	0.0	0.0
12	2.2143587881257224E-4	0.0	0.0	0.0	0.0
13	2.2143587881257224E-4	0.0	0.0	4.428717576251445E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACC	25	0.0060306676	18.997229	12
TCTTCGC	25	0.0060306676	18.997229	8
AGGCCTC	35	0.0021674957	16.285145	17
TCAACGG	35	0.0021674957	16.285145	5
TCCCGAC	40	0.0052726422	14.249501	2
CAAGACG	50	0.0014970663	13.299534	4
GTTATTC	50	0.0014970663	13.299534	3
AAGACGG	55	0.003063447	12.090486	5
CCGGAAT	80	2.8568282E-5	11.874585	3
GTAAAAA	90	7.3030988E-6	11.628755	1
ATTCCCC	90	7.43055E-6	11.610704	19
GACGGAC	60	0.005871078	11.081717	7
TTACCCG	60	0.005871078	11.081717	13
ATCGAAC	90	9.493877E-5	10.554016	8
CGAACCC	100	2.3918803E-5	10.448476	10
AATCGAA	100	2.3918803E-5	10.448476	7
TAAATGA	75	0.0026421128	10.132978	4
GAACCCT	95	1.6403702E-4	9.998541	11
CCCTGAT	100	2.7460756E-4	9.499667	14
GATTCCC	100	2.7460756E-4	9.499667	18
>>END_MODULE