FastQCFastQC Report
Wed 25 May 2016
SRR1294877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451598
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC108452.401472105722346No Hit
GTACATGGGAAGCAGTGGTATCAAC96622.139513461087073No Hit
GTATCAACGCAGAGTACTTTTTTTT56511.2513341511698457No Hit
GAGTACATGGGAAGCAGTGGTATCA47621.054477654905469No Hit
CATGTACTCTGCGTTGATACCACTG45241.001775915748077No Hit
TATCAACGCAGAGTACTTTTTTTTT38660.8560711074894043No Hit
GGTATCAACGCAGAGTACTTTTTTT38560.8538567487012786No Hit
GCGTTGATACCACTGCTTCCCATGT33270.7367171688094278No Hit
ACGCAGAGTACATGGGAAGCAGTGG30060.6656362517105922No Hit
TATCAACGCAGAGTACATGGGAAGC26400.5845907200651907No Hit
ACTCTGCGTTGATACCACTGCTTCC25650.5679830291542478No Hit
GTATCAACGCAGAGTACATGGGAAG23100.5115168800570419No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22520.49867359908591274No Hit
GGTATCAACGCAGAGTACATGGGAA22220.49203052272153547No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18700.4140850933795101No Hit
GTGGTATCAACGCAGAGTACATGGG17310.38330550622456255No Hit
GCTTCCCATGTACTCTGCGTTGATA16730.37046222525343336No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14890.3297180235519201No Hit
ATACCACTGCTTCCCATGTACTCTG14220.3148818196714777No Hit
GTACTCTGCGTTGATACCACTGCTT13810.3058029486401623No Hit
CAGTGGTATCAACGCAGAGTACATG13660.3024814104579737No Hit
GATACCACTGCTTCCCATGTACTCT12800.2834379248800925No Hit
GGGAAGCAGTGGTATCAACGCAGAG12650.2801163866979039No Hit
GCAGAGTACATGGGAAGCAGTGGTA12420.2750233614852147No Hit
CTGCTTCCCATGTACTCTGCGTTGA12290.2721446950606513No Hit
CATGGGAAGCAGTGGTATCAACGCA12120.26838028512083756No Hit
GCAGTGGTATCAACGCAGAGTACAT12120.26838028512083756No Hit
TACCACTGCTTCCCATGTACTCTGC10930.24202941554214147No Hit
GTTGATACCACTGCTTCCCATGTAC10890.24114367202689116No Hit
CCACTGCTTCCCATGTACTCTGCGT10330.2287432628133871No Hit
CTTCCCATGTACTCTGCGTTGATAC9550.2114712642660065No Hit
ACATGGGAAGCAGTGGTATCAACGC9180.20327813674994133No Hit
AAGCAGTGGTATCAACGCAGAGTAC8980.1988494191736899No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8890.1968564962643767No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8760.1939778298398133No Hit
ATGGGAAGCAGTGGTATCAACGCAG7240.1603195762603023No Hit
CCATGTACTCTGCGTTGATACCACT7060.156333730441676No Hit
TGATACCACTGCTTCCCATGTACTC6630.1468119876527354No Hit
ATCAACGCAGAGTACTTTTTTTTTT6540.14481906474342227No Hit
GTGGTATCAACGCAGAGTACTTTTT6060.13419014256041878No Hit
TTCCCATGTACTCTGCGTTGATACC5670.12555414328672845No Hit
GTACATGGTAAGCAGTGGTATCAAC5530.12245404098335244No Hit
ATCAACGCAGAGTACATGGGAAGCA5300.1173610157706633No Hit
ACCATGTACTCTGCGTTGATACCAC4710.10429629892072152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA350.002163114716.2900814
GTATTAT508.631367E-515.2125031
GTATAAA456.707235E-414.7899331
TGGACTG456.735479E-414.7817415
GTACTTA400.005242744514.2617221
GGCTAGG500.001487427313.3109411
CCGCCTG500.001498649113.29767419
AAGTACT655.4221273E-513.1573744
GTTCAAA604.053817E-412.6770851
CAAAGCA751.4690324E-512.6700644
CTGAAGT550.003056505512.09415154
AGGCCCG550.003066667112.08879510
ACTGACT657.9860975E-411.6954433
AGCGTGT658.0158445E-411.69026415
CATACTC907.405555E-611.6142253
ACTCCCC907.405555E-611.6142256
TGAAGTG855.288786E-511.1794685
AGCTGTG855.3127173E-511.1745177
GTCCTGG600.005828929611.0924491
CCGAGCC600.00587236511.08139514
Produced by FastQC (version 0.11.5)