FastQCFastQC Report
Wed 25 May 2016
SRR1294876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187935
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT400.00528348114.24902317
GTACGAC658.019341E-411.6938823
CGGTTTA700.001493170510.85639915
GGTACGA750.002646217610.1346972
GGTATCA14850.09.8667381
GTATCAA28750.09.5309011
TAGACAG5650.09.2475965
GTATTAG5950.08.9546871
CTTATAC5600.08.8226163
GTGCTAG3900.08.7824811
ACACCGT1951.6572812E-88.7673726
GTCTTAC4350.08.7488321
GGACCGA1201.7691284E-48.7064896
TACACCG1107.1501755E-48.6361845
AGGACTG6850.08.5983615
GTAGTAC3650.08.5901983
GTATAGG5650.08.5881791
GTCTTAG3550.08.576321
GTACGGA1000.00286707248.562921
ATAGGAC3900.08.5267893
Produced by FastQC (version 0.11.5)