FastQCFastQC Report
Wed 25 May 2016
SRR1294876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187935
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82990.19816448918142235No Hit
GGTATCAACGCAGAGTACTTTTTTT49210.11750421150280507No Hit
TATCAACGCAGAGTACTTTTTTTTT46160.11022138595751843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGC456.763008E-414.7785586
TACGTCG658.0248533E-411.69292455
GGTATCA20350.011.678771
ACCCGTG1106.058508E-610.3626997
GTATCAA38750.09.9358441
CGGATAG800.00452152179.4991412
GTACCGC1054.493122E-49.0480976
GTCTTAC4950.09.0263911
TGCGTTG4000.09.02418312
GTGTTAG6450.08.9906611
CCTATAC4450.08.9670873
ACAGCGT1701.6895319E-78.9403688
TACCGCT850.00744467238.9403687
GTCTTAG4700.08.8997191
GCACCGT1304.369644E-58.7696936
CGAGTGC1304.377E-58.76843710
GTCTTAA4600.08.4732011
AGGACCG1356.7991205E-58.444895
GTCTAGG5300.08.4303081
CTTAGAC2606.002665E-118.4045913
Produced by FastQC (version 0.11.5)